Morphological and histological changes during somatic embryo formation from coconut plumule explants

Sáenz, Luis; Azpeitia, A.; Chuc-Armendariz, B.; Chan, José Luis; Verdeil, Jean‐Luc; Hocher, Valérie; Oropeza, Carlos

doi:10.1079/ivp2005728

Cited by 43 publications

(29 citation statements)

References 18 publications

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“…1A). Similar results have been reported for coconut (Sáenz et al, 2006) and papaya sections (Almeida et al, 2001). Embryogenic callus can be obtained from floral shoots of Heliconia stricta after incubation in MS medium supplemented with 13.57 to 22.62µM 2,4-D and 5.71 to 14.27µM IAA for four to six months .…”

Section: Resultssupporting

confidence: 89%

“…1B). Cells that demonstrated these characteristics were considered embryogenic cells (Durzan, 1988;Mathew and Philip, 2003;Kim et al, 2003;Sáenz et al, 2006) and staining with acetic carmine indicated the presence of reserve substances that would favor tissue development (Guerra et al, 1999). The combination of IAA and 2,4-D also promoted the development of yellowish nodules, especially in the treatments with 8.56 µM IAA plus 13.57 µM 2,4-D; 11.42 µM IAA plus 22.62 µM 2,4-D; and 14.27 µM IAA plus 22.62 µM 2,4-D. Histological analyses of this callus material revealed heavily stained cells located on the surface of the callus.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

Ulisses

Câmara

Willadino³

et al. 2010

Braz. arch. biol. technol.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

Ulisses

Câmara

Willadino³

et al. 2010

Braz. arch. biol. technol.

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show abstract

“…Several of them could germinate and form plantlets that were successfully transferred into ex vitro conditions. The pattern of development of embryogenic callus described above is highly reproducible, each time showing the same sequence of events, associated with the corresponding subcultures in specific media as described by Sáenz et al (2006) and Pérez-Nuñez et al (2006).…”

Section: Histological and Ultrastructural Changes During Somatic Embrmentioning

confidence: 87%

“…The evidence presented and supported a multicellular origin of somatic embryos. Sáenz et al (2006) also studying somatic embryogenesis from plumule explants excised from Malayan Green Dwarf coconut seed, reported a detailed histological study of the development of embryogenic callus and regeneration from it ( Fig. 18.2).…”

Section: Histological and Ultrastructural Changes During Somatic Embrmentioning

confidence: 99%

Somatic Embryogenesis in Cocos nucifera L.

Sáenz

Montero-Cortés

Pérez-Nuñez

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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In our coconut laboratory micropropagation has been the subject of research for nearly three decades, as this plant species is highly recalcitrant for in vitro regeneration and so far only achieved through somatic embryogenesis as the sole path for coconut regeneration. Of all the explants tested, plumules have proved to be the most responsive and the process efficiency has been improved by indirect embryogenesis and thereafter secondary embryogenesis and callus multiplication, this strategy is currently applied in floral explants. Two different approaches have been used to find ways to have a more efficient protocol. The first one, a direct and practical method, included plant hormones and activated charcoal. On the other hand, the indirect approach consisted in basic studies on: morphohistological development, biochemical and physiological aspects such as uptake of exogenous auxin, levels of endogenous auxin; shoot apical meristem formation and maintenance (KNOX gene family); the occurrence and expression of genes related to the cell cycle control (Cyclin-Dependent Kinase), and somatic embryogenesis (Somatic Embryogenesis-Related Kinase); and the establishment of a transformation protocol. A better understanding of the somatic embryogenesis of coconut was achieved by these approaches. This way, in the short term there is no doubt that we will have mass propagation options based not only in plumule explants but also on rachillae, unfertilized ovary, and leaf explants.

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“…Out of three types of plumule extraction, direct plumule extraction from fresh embryos were found to be more effective in initial calli (79%) and formation of embryogenic calli (56%). Most of the translucent calli showed ear-like shape (Saenz et al, 2006). Chan et al (1998) also reported the direct excision of plumule from fresh embryos which resulted in 54.3% of embryogenic calli formation in Y3 media supplemented with 2, 4-D (100 µM).…”

Section: Effect Of Three Different Methods Of Shoot Meristem Excisionmentioning

confidence: 95%

A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut

Bhavyashree¹,

K²,

Fayas³

et al. 2016

RIB

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A protocol was standardized to maximize yields of embryogenic calli from shoot meristem culture of coconut. Three different shoot meristem excision methods were tested viz., excision of shoot meristem aseptically from in vitro germinated embryo after 10-12 days, excision of shoot meristem from in vitro germinated embryo subjected to GA3 treatment for five days and excision of shoot meristem from fresh embryo. The primary calli induction after 30 days of culture incubation for the three treatments was 21%, 27% and 79% respectively. Further, the primary calli formed from the shoot meristem excised from fresh embryo gave rise to 56% of embryogenic calli. The calli obtained from the shoot meristem, which were excised from in vitro germinated embryo, formed less percentage of embryogenic calli because of the presence of cotyledonary tissues which inhibited the multiplication of meristematic tissues. In the case of shoot meristem extracted from GA3-treated embryos, the percentage of non-embryogenic calli was more compared to the shoot meristem excised from fresh embryo. It was observed that the addition of GA3 in the initial stages of culture inhibited the formation of embryogenic calli and favored direct shoot development. Currently, the shoot meristem excised from fresh embryo is being employed for scaling up the planting material production from released varieties of coconut.

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Morphological and histological changes during somatic embryo formation from coconut plumule explants

Cited by 43 publications

References 18 publications

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

Somatic Embryogenesis in Cocos nucifera L.

A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut

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