Morphological and immunological characterization of immunostimulatory complexes based on glycoglycerolipids from Laminaria japonica.

Abstract

Some physicochemical properties of glycoglycerolipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol) from the sea algae Laminaria japonica, as well as their ability to become incorporate into immunostimulating complexes (ISCOMs), used as a delivery system of microbial and tumor antigens in vesicular form, were studied. These glycolipids were found to differ essentially in fatty acid composition, unsaturation index and thermotropic behavior. The possibility of ISCOM modification by embedding the glycolipids studied instead of a phospholipid component in vesicles was shown. A preliminary research of the immunogenicity of the pore-forming protein from Yersinia pseudotuberculosis in modified (by monogalactosyldiacylglycerol) and typical (egg phosphatidylcholine) ISCOMs did not reveal a significant enhancement of immune response in comparison with that of isolated protein.

Biomarker-Guided Screening of Juzen-taiho-to, an Oriental Herbal Formulation for Immunostimulation

“…β-Glucosylceramides have been shown to serve as immunoadjuvants for HBV and cancer vaccinations in preclinical studies [27,28]. In addition, DGDGs are known to possess immunomodulatory effects [29]. Here, it is important to note that the compounds identified in the current study are major constituents in the glycolipid mixture.…”

Tubular immunostimulating complex based on cucumarioside A2-2 and monogalactosyldiacylglycerol from marine macrophytes

“…Along the ordinate, percentages with respect to the value for an anti-porin reference serum (serum of mice immunized with pure porin). Along the horizontal axis, the experimental group of animals immunized with: FCA+porin - porin mixed with Freund's complete adjuvant (Sigma); ISCOM+porin - porin incorporated in ISCOMs, prepared as described [ 11 ]; TI(PC)+porin - porin incorporated in TI-complex consisting of a cucumarioside A 2 -2, cholesterol and PC from egg yolk at weight ratio of 3:2:6; TI+porin - porin incorporated in TI-complexes consisting of a cucumarioside A 2 -2, cholesterol and MGalDG from Ulva fenestrata at weight ratio of 3:2:6. Dose of antigen (denaturated monomeric porin YompF) - 10 μg/mouse.…”

“…The resulted mixture was sonicated by ultrasonic disintegrator SONOPULS Ultrashall-Homogenizatoren HD 2070 (Germany) at 10% maximum power (0.7 sec - work; 0.3 sec - interval) [ 13 - 15 ]. Porin-containing ISCOMs were prepared as described [ 11 ].…”

“…However, we proposed principally new biologically active components, glycoglycerolipids and triterpene glycosides isolated from marine macrophytes and invertebrates, respectively, to modify and optimize ISCOM vehicles for microbial antigens. The immunologically inert phospholipid phosphatidylcholine (PC) of ISCOMs was replaced by glycolipid monogalactosyldiacylglycerol (MGalDG) from marine macrophytes (algae and seagrasses) [ 11 ]. Subsequent substitution of the sum of saponins from Q. saponaria by triterpene glycosides from sea cucumbers, possessing immunostimulating activity, resulted in a tubular superstructure of the complexes (TI-complexes) instead of the vesicular one of classical ISCOMs [ 12 ].…”