Morphological and molecular characterisation of Xiphinema zagrosense sp. n. (Dorylaimida: Longidoridae) from the Zagros Mountains, Iran

Ghaemi, Razieh; Pourjam, Ebrahim; Pedram, Majid; Robbins, R. T.; Ye, Weimin; Decraemer, Wilfrida

doi:10.1163/156854111x612171

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…The abovementioned four species form a clade with X. diversicaudatum (EF538755, AY601624), X. bakeri (AY601623), X. globosum (GU549474), X. abrantinum (AY601625) and X. index Thorne & Allen, 1950 (AY601628, HM921364) with a 1.00 BPP and 88% ML BS support. The phylogenetic tree shows morphospecies group 5 to be polyphyletic, a result in agreement with the observations in Ghaemi et al (2012).…”

Section: Diagnosis and Relationshipssupporting

confidence: 90%

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Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships

Roshan-Bakhsh

Pourjam

Pedram

et al. 2014

Nematol

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Xiphinema castilloi sp. n., a new species of morphogroup 5 sensu Loof & Luc, 1990, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by a 4.2-5.0 mm hody assuming an open C to J shape after heat fixation, lip region separated from the body by a shallow depression, a 101-126 /xm odontostyle, two equally developed genital tracts with pseudo-Z-organ in uterus (8-11 globular structures) and devoid of spines, tail dorsally convex conoid with a muero, presence of functional males, and four Juvenile developmental stages. By having two equally developed genital tracts, presence of pseudo-Z-organ, and similarities in tail shape of females and juveniles, the new species comes more close to X. artemisiae, X. diversicaudatum, X. erriae, X. jomercium, X. lapidosum, X. lusitanicum and X. ripogranum. The Bayesian inference (BI) and Maximum Likelihood (ML) analyses using 707 bp partial sequences of D2-D3 segment of 28S rDNA placed the new species in a monophyletic group with X. dentatum, another member of morphospecies group 5 with 1.00 Bayesian posterior probability (BPP).

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Section: Diagnosis and Relationshipssupporting

confidence: 90%

“…For both Bl and ML methods, Nevadanema nevadense (JN242245) was used as outgroup. As discussed by Pedram et al (2012b) and Ghaemi et al (2012), to examine the nature of differentiation in the uterus (presence/absence of spines) fresh females in temporary slides in water were studied.…”

Section: Methodsmentioning

confidence: 99%

Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships

Roshan-Bakhsh

Pourjam

Pedram

et al. 2014

Nematol

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“…mazandaranense Pedram, Pourjam, Robbins, Ye, Atighi & Decraemer 2012 [37], X . zagrosense Ghaemi, Pourjam, Pedram, Robbins, Ye & Decraemer 2012 [54] and X . castilloi Roshan-Bakhsh, Pourjam, Pedram, Robbins & Decraemer 2014 [23]; all of which were characterized with molecular data in their original description, except X .…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, and description of a new dagger nematode, Xiphinema afratakhtehnsis sp. nov., (Dorylaimida: Longidoridae) in natural forests of southeastern Gorgan, northern Iran

et al. 2019

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The most prevalent dagger nematode recovered from rhizospheric soil samples of forest trees in the Afrātakhteh region of Golestan province (Iran) was Xiphinema afratakhtehnsis sp. nov. and it is described and illustrated with integrative approaches using both morphological and molecular criteria. It belongs to the morphospecies group 6 of the intragenic historical grouping of Xiphinema non- americanum species. The new species is characterized by females with 3.3–4.9 mm sized body, lip region separated from the rest of body by a depression, anteriorly expanded, 16–18 μm wide, vulva located at 47.2–58.5%, odontostyle 155–173 μm and odontophore 89–107 μm long, female genital system composed of two equally developed branches, the tubular part of each having spines, short symmetrically rounded female tail to symmetrically rounded with a small mucro-like projection at the end in a few females, rare males (n = 1 out of 74 females) with 83 μm long dorylaimoid spicules and four juvenile developmental stages. The third-stage juveniles (J3) have a characteristic tail shape (short, symmetrically conical with a club-shaped long mucro) demarcating the species, and being typologically useful for its separation from closely similar species (except X . cohni , with currently no data on its juvenile stages) viz . X . adenohystherum , X . iranicum , X . mazandaranense , X . nuragicum , X . pyrenaicum , X . robbinsi , X . sphaerocephalum and X . zagrosense . Molecular phylogenetic studies using genomic (partial large subunit and internal transcribed spacer 1 ribosomal RNA genes: D2-D3 and ITS1 rDNA) and mitochondrial cytochrome c oxidase subunit I gene ( COI mtDNA) revealed the new species forming a unique lineage in all reconstructed trees using Bayesian inference (BI) and maximum likelihood (ML) methods. The sequenced isolates of the new species formed a monophyletic group in the D2-D3 tree. The sequenced isolates of the new species for their COI mtDNA formed four subclades in COI mtDNA phylogeny, and four haplotypes in corresponding analysis.

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“…), secretions of the uterine wall that can be small to large and can be inside the globular bodies or more free in the uterine duct. Finally, their presence or absence is not a dependable criterion for separating species -for better observation of uterine inclusions we studied fresh female specimens on temporary slides (Ghaemi et al, 2012;Pedram et al, 2012b). This was documented in our present study using morphological, morphometric and molecular data.…”

Section: Remarks On the Pseudo-z-organmentioning

confidence: 83%

Molecular characterisation of, and new data on, two populations of Xiphinema ingens Luc & Dalmasso, 1964 (Nematoda: Longidoridae) from Iran

Mirzaei

Pourjam

Pedram

2015

Nematol

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Two populations of Xiphinema ingens were recovered and characterised based on morphological, morphometric and molecular data. Interesting morphological variation was observed on the nature of differentiation in uterus of females between both populations, i.e. one population had only a pseudo-Z-organ in the shape of globular bodies, whilst the second population had a similar pseudo-Z-organ but also had crystalloids which varied in size and number and were located near the pseudo-Z-globules or sometimes at some distance from them towards the vagina. Variation was also observed in the shape of tail of juveniles within each population as well as between two recovered populations. Both populations had the same range of morphometric data and formed a fully supported clade in both Bayesian inference (BI) and maximum likelihood (ML) methods of phylogenetic analyses using partial sequences of 28S rDNA D2-D3 and ITS1 regions. The two populations of X. ingens formed a clade with another Xiphinema species native to Iran (X. castilloi) in 28S and two species, X. macroacanthum and X. bernardi, in ITS1 trees.

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Morphological and molecular characterisation of Xiphinema zagrosense sp. n. (Dorylaimida: Longidoridae) from the Zagros Mountains, Iran

Cited by 8 publications

References 13 publications

Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships

Description of Xiphinema castilloi sp. n. (Dorylaimida: Longidoridae) from Iran and its phylogenetic relationships

Genetic diversity, and description of a new dagger nematode, Xiphinema afratakhtehnsis sp. nov., (Dorylaimida: Longidoridae) in natural forests of southeastern Gorgan, northern Iran

Molecular characterisation of, and new data on, two populations of Xiphinema ingens Luc & Dalmasso, 1964 (Nematoda: Longidoridae) from Iran

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