Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Handoo, Zafar A.; Carta, Lynn K.; Skantar, Andrea M.

doi:10.1163/156854101753389220

Cited by 26 publications

(13 citation statements)

References 16 publications

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“…Pratylenchus zeae was also identified by its three lip annuli and bluntly pointed tail. The D3 region DNA sequence of this population matched that for P. zeae available from GenBank (AF303950; Carta et al, 2001). Pratylenchus hexincisus was common at the type locality (Table 1; Figs 1A-J; 2A, D).…”

Section: Root-lesion Nematodes Foundmentioning

confidence: 57%

Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus

Inserra

Troccoli²,

Gözel

et al. 2007

Nematol

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A new root-lesion nematode Pratylenchus hippeastri n. sp. is described from amaryllis (Hippeastrum spp.) in Florida. The new species is characterised by a combination of the following morphological features of females: slender body, flat, plain and smooth face, head with two lip annuli, an incomplete third annulus, and with second lip annulus thicker than first, ellipsoidal stylet knobs, rectangular empty spermatheca with large round cavity, and conoid tail with bluntly pointed terminus usually showing a ventral constriction, or subhemispherical and smooth. The ranges of morphometric characters of P. hippeastri n. sp. overlap with those of P. scribneri and P. hexincisus. These three species also share some morphological and biological features such as two lip annuli, an empty spermatheca and similar lateral fields, but differ in the morphology of head patterns (smooth face in P. hippeastri n. sp. vs divided face in the others) and tail termini (hemispherical and subhemispherical in P. scribneri and subdigitate in P. hexincisus vs bluntly pointed in P. hippeastri n. sp.). A reference population of P. scribneri from Ohio and one of P. hexincisus from Tennessee were obtained during the course of unsuccessful attempts to recover P. scribneri at the type locale in Tennessee. The D3 rDNA sequences of the reference populations matched GenBank sequences for these two species. Small morphological differences between the P. scribneri population from Ohio and the P. scribneri lectotype occurred in the shape of stylet knobs (ellipsoidal vs round) and spermatheca (round with a central cavity vs oblong) and also in the length of the pharyngeal overlap (longer in the reference population). These morphological differences cast doubt about the identity of the lectotype, which may be P. hexincisus. New morphological details were obtained for P. hexincisus from Tennessee, which included a divided face, an almost rectangular empty spermatheca with a central cavity and subdigitate tail termini. Based on our phylogenetic inferences from DNA sequences P. hippeastri n. sp. is more closely related to P. zeae and a Florida population of P. loosi than to P. scribneri or P. hexincisus.Amaryllis (Hippeastrum spp.) are flowering ornamentals that beautify gardens throughout Florida and warm states in the United States. These bulbous plants are native in Tropical America. Intense breeding programmes by amaryllis growers have improved the size, colour and shape of the flowers, and resulted in a great number of varieties that are marketed by ornamental industries in many

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Section: Root-lesion Nematodes Foundmentioning

confidence: 57%

Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus

Inserra

Troccoli²,

Gözel

et al. 2007

Nematol

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“…Ribosomal DNA (rDNA) sequence has been shown to be a reliable genetic marker for identifi cation purposes, and a powerful tool to analyze genetic variation. The 28S D2/D3 rDNA fragment has been used frequently to characterise Pratylenchus populations (Handoo et al, 2001;Al-Banna et al, 2004;De Luca et al, 2004;Inserra et al, 2007;. Previously P. neglectus has been found under the rhizosphere of Brassica (Kumari, 2012) and Pratylenchus sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pratylenchus crenatus and P. neglectus (Nematoda: Pratylenchidae) associated with wheat crop

Kumari

2015

Helminthologia

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SummaryThe distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identifi ed based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in fi ve localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while fi ve populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).

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“…Al-Banna et al (1997) was the first to conclude, based on parsimony analysis of D3 expansion region of 28S rRNA gene sequences for ten Pratylenchus species together with Nacobbus, Radopholus and Hirschmanniella, that Pratylenchus was a paraphyletic assemblage. The paraphyly of Pratylenchus can also be observed on some other phylogenetic trees derived from more comprehensive analysis (see Carta et al, 2001;De Luca et al, 2004) using same gene fragment. However, the question concerning Pratylenchus paraphyly was not discussed by these authors, perhaps because of a lack of statistical support for such a conclusion.…”

Section: Monophyly Of the Genus Pratylenchusmentioning

confidence: 67%

“…(Al-Banna et al, 1997;Duncan et al, 1999;Carta et al, 2001;De Luca et al, 2004;Subbotin et al, 2008). These studies have clarified the taxonomical status of a large number of root-lesion nematodes, although many species have yet to be so characterised.…”

mentioning

confidence: 99%

Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae

Palomares-Rius

Castillo

Liébanas³

et al. 2010

Nematol

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A new amphimictic species, Pratylenchus hispaniensis n. sp., parasitising the roots of gum cistus in Andújar (Jaén), southern Spain, is described. The new species is characterised by the presence of numerous males and by the female having a lip region with three annuli, a divided face, a robust stylet (14.5-17.0 μm) with rounded knobs, lateral fields with four lines, V = 80-84, a round spermatheca full of sperm, well developed post-vulval uterine sac and an obliquely truncate tail with irregularly annulated terminus. Morphologically this species is related to P. bhatti, P. kralli, P. mediterraneus, P. pseudofallax and P. thornei. A phenetic study of the 25 most useful diagnostic morphological and allometric characters for Pratylenchus species was done using multivariate factor and linear discriminant analyses. In the factor analysis the first seven factors accounted for 71.1% of the total variance of the characters selected. These factors were related to female tail, pharyngeal overlap, reproductive behaviour, stylet length, L/post-vulval uterine sac ratio, body length and number of lip annuli. Discriminant analysis differentiated Pratylenchus spp. from the three valid species of Zygotylenchus. The results of the phylogenetic analysis based on sequences of the D2-D3 expansion regions of 28S, partial 18S and ITS rRNA genes confirmed the close relationship of P. hispaniensis n. sp. with P. mediterraneus and inferred molecular affinity with P. brzeskii, P. neglectus and P. thornei, in spite of variation in the position of P. hispaniensis n. sp. in the clades. Additional phylogenetic analyses based on the same sets of sequences for P. hispaniensis n. sp., Zygotylenchus guevarai and other Pratylenchidae indicated that Pratylenchus includes several paraphyletic lineages; however, likelihood tests did not reject monophyly of the genus. The inclusion of Pratylenchus, Zygotylenchus, Hirschmanniella, Nacobbus and Apratylenchus in Pratylenchidae was supported.The genus Pratylenchus Filipjev, 1936 contains more than 70 species of root-lesion nematodes. The morphological identification and delimitation of these species remains problematic due to their high morphological plasticity, the small number of diagnostic features available at species level, the intraspecific variability of some of

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Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P.convallariae and P.fallax (Nematoda: Pratylenchidae)

Cited by 26 publications

References 16 publications

Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus

Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus

Characterization of Pratylenchus crenatus and P. neglectus (Nematoda: Pratylenchidae) associated with wheat crop

Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae

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