Morphological and molecular characterization of two graminicolous &lt;i&gt;Exserohilum&lt;/i&gt; species associated with cultivated rice and early barnyard grass from Sri Lanka

Ferdinandez, Himashi S.; Manamgoda, Dimuthu S.; Udayanga, Dhanushka; Deshappriya, N.; Munasinghe, M. L. A. M. S.

doi:10.4038/cjs.v49i5.7805

Cited by 2 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colonies were observed at 2–3 day intervals until full growth was attained. Small portions of fungal mycelium from fully grown culture were aseptically transferred to plates of fresh PDA culture medium in order to obtain pure cultures of E. turcicum [ 16 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…The growing cultures in Petri plates were aseptically opened under laminar air flow, clean microscope glass slides were gently placed over the surface of the colonies, touching the edge, and the plates were resealed, followed by incubation under continuous light to produce spores. A total of 45 fungal isolates were studied for morphological and cultural characteristics after the culture growth reached 10 days [ 18 , 19 ]. The color and texture of the colonies as well as growth pattern, pigmentation, and margin growth were observed [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

et al. 2022

View full text Add to dashboard Cite

Maize is considered the third most important cereal crop in Asia after rice and wheat. Many diseases affect this crop due to the cultivation of various hybrids. This research aimed to characterize the causative agent of northern corn leaf blight disease in Bihar, India, caused by Exserohilum turcicum (Passerini) Leonard and Suggs. Leaf samples were collected from infected fields in five maize growing districts of Bihar in 2020–2022. A total of 45 fungal isolates from 135 samples were examined for cultural, morphological, and molecular characteristics and were identified as E. turcicum. The isolates were grouped into four groups based on colony color, i.e., olivaceous brown, blackish brown, whitish black, and grayish, and into two groups based on regular and irregular margins. The conidial shapes were observed to be elongated and spindle-shaped with protruding hilum, with conidial septa ranging from 2–12. Similarly, conidial length varied from 52.94 μm to 144.12 μm. β-tubulin gene sequences analysis made it possible to verify the identities of fungal strains and the phylogenetic relationships of all isolates, which were clustered in the same clade. The β-tubulin gene sequences of all the isolates showed a high level of similarity (100%) with reference isolates from GenBank accession numbers KU670342.1, KU670344.1, KU670343.1, KU670341.1, and KU670340.1. The findings of this study will serve as a baseline for future studies and will help to minimize yield losses.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Raw sequences generated for fresh isolates were assembled with BioEdit v7.0.5 for Windows. To confirm the identity, phylogenetic analyses were performed using both Maximum Parsimony (MP) and Maximum Likelihood (ML) criteria (Ferdinandez et al, 2020) along with ex-type and reference DNA sequences of the genus Curvularia (Ferdinandez et al, 2021).…”

Section: Sequence Alignment Phylogenetic Analyses and Species Recogni...mentioning

confidence: 99%

First report of Curvularia dactylocteniicola causing leaf spots on Zea mays and Sorghum in Sri Lanka

Ferdinandez,

Manamgoda,

Udayanga

2024

Ceylon J. Sci.

View full text Add to dashboard Cite

The genus Curvularia includes pathogens, endophytes and saprobes associated with a diverse range of plants belonging to the family Poaceae. These species are responsible for diseases on cereal crops such as rice, maize, wheat, millet, sorghum and poaceous weedy hosts. During a field survey of cereal pathogens conducted in Galle, Hambanthota, and Matale districts of Sri Lanka, diseased leaf samples were collected randomly from Zea mays and an indigenous cereal landrace of Sorghum sp. traditionally known as ‘Swayanjatha’. Morphology and molecular phylogeny based on combined nuclear ribosomal internal transcribed spacers 1 and 2 with 5.8S region (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and translation elongation factor 1-α (TEF1) revealed that the causative agent of the leaf spots is Curvularia dactylocteniicola. Results of the pathogenicity tests confirmed cause of the disease on Zea mays and Sorghum sp. To the best of our knowledge, this is the first record of C. dactylocteniicola causing leaf spots on Zea mays and Sorghum sp. in Sri Lanka.

show abstract

Morphological and molecular characterization of two graminicolous <i>Exserohilum</i> species associated with cultivated rice and early barnyard grass from Sri Lanka

Cited by 2 publications

References 16 publications

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

First report of <em>Curvularia dactylocteniicola</em> causing leaf spots on <em>Zea mays</em> and <em>Sorghum</em> in Sri Lanka

Contact Info

Product

Resources

About