Morphological and molecular characterization of human gastric mucous cells in long-term primary culture

Wagner, Siegfried; Enss, Marie-Luise; Cornberg, Markus; Mix, Heiko; Schumann, Silke; Kirchner, Gabriele I.; Jähne, J.; Manns, Michael P.; Beil, Winfried

doi:10.1007/s004240050717

Cited by 10 publications

(9 citation statements)

References 40 publications

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“…21 Informed consent was obtained from each patient, according to the revised version of the Declaration of Helsinki. In addition, the study was approved by the local ethics committee of the Medizinische Hochschule Hannover.…”

Section: Methodsmentioning

confidence: 99%

Expression of leptin and leptin receptor isoforms in the human stomach

Mix

Widjaja²,

Jandl³

et al. 2000

Gut

168

102

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Background-Leptin is an important regulator of food intake and energy expenditure. Initially it was thought to be expressed exclusively in and secreted by adipocytes. Recently, leptin expression was also noted in other tissues, including rat gastric mucosa. Information on leptin and leptin receptor expression in the human stomach is lacking. Aim-To investigate expression of leptin and its corresponding receptors in human gastric epithelial cells. Methods-Fundic and antral gastric mucosal biopsies, primary cultures of human gastric epithelial cells, and the human gastric cancer cell line AGS were screened for expression of leptin and diVerent leptin receptor isoform mRNA by reverse transcriptase-polymerase chain reaction. Immunohistochemistry was performed for localisation of leptin and leptin receptor proteins in gastric mucosa. Results-mRNA of leptin and its four receptor isoforms (huOB-R, long receptor isoform; huB219.1-3, short receptor isoforms) was detected in gastric mucosal biopsies, cultured human gastric epithelial cells, and gastric cancer cells. Immunohistochemistry demonstrated that chief as well as parietal cells were reactive to leptin and leptin receptors. Conclusions-Leptin and leptin receptors are expressed in human gastric mucosa. These findings suggest a paracrine and/or autocrine eVect of leptin on gastric epithelial cell function. (Gut 2000;47:481-486)

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Section: Methodsmentioning

confidence: 99%

Expression of leptin and leptin receptor isoforms in the human stomach

Mix

Widjaja²,

Jandl³

et al. 2000

Gut

168

102

View full text Add to dashboard Cite

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“…For example, GlcNAc is a common component of chitin and mucinlike proteins in Drosophila (45-47) as well as mucin, hyaluronic acid, and serum in mammals (48)(49)(50)(51). Indeed, chitin and mucin both stimulate pyocyanin production in P. aeruginosa (Fig.…”

Section: Discussionmentioning

confidence: 99%

Community surveillance enhances Pseudomonas aeruginosa virulence during polymicrobial infection

Korgaonkar

Trivedi

Rumbaugh

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

296

284

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Most infections result from colonization by more than one microbe. Within such polymicrobial infections, microbes often display synergistic interactions that result in increased disease severity. Although many clinical studies have documented the occurrence of synergy in polymicrobial infections, little is known about the underlying molecular mechanisms. A prominent pathogen in many polymicrobial infections is Pseudomonas aeruginosa, a Gram-negative bacterium that displays enhanced virulence during coculture with Grampositive bacteria. In this study we discovered that during coinfection, P. aeruginosa uses peptidoglycan shed by Gram-positive bacteria as a cue to stimulate production of multiple extracellular factors that possess lytic activity against prokaryotic and eukaryotic cells. Consequently, P. aeruginosa displays enhanced virulence in a Drosophila model of infection when cocultured with Gram-positive bacteria. Inactivation of a gene (PA0601) required for peptidoglycan sensing mitigated this phenotype. Using Drosophila and murine models of infection, we also show that peptidoglycan sensing results in P. aeruginosa-mediated reduction in the Gram-positive flora in the infection site. Our data suggest that P. aeruginosa has evolved a mechanism to survey the microbial community and respond to Gram-positive produced peptidoglycan through production of antimicrobials and toxins that not only modify the composition of the community but also enhance host killing. Additionally, our results suggest that therapeutic strategies targeting Gram-positive bacteria might be a viable approach for reducing the severity of P. aeruginosa polymicrobial infections.Staphylococcus | quorum sensing | PQS | glucosamine

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“…Mucins secreted by 10 6 cells in response to one of the cytokines were isolated and quantitated as described previously [32,33]. In brief, subsequent to centrifugation, medium of a single culture was concentrated by ultrafiltration (excl.…”

Section: Quantitation and Characterization Of Secreted Mucinsmentioning

confidence: 99%