Morphological and Molecular Characterization of Coccidiosis in Local Chickens of Mekong Delta in Vietnam

Nguyen, Hung Huu; Le, Trung; Lu, Tien Ai; Nguyen-Ho-Bao, Tran

doi:10.36380/jwpr.2021.59

Cited by 3 publications

(3 citation statements)

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“…E. acervulina usually has been slightly pathogenic at an initial infection, and its pathogenicity on the examined chickens is dependent on the number of ingested oocysts. Similar patterns have been documented in earlier research on chickens (Allen & Fetterer, 2000;Idriss et al, 2019;Jatau et al, 2016;Nguyen et al, 2021). Idriss et al (2019).…”

Section: Discussionsupporting

confidence: 88%

“…In comparing the present Eimeria with E. acervulina of other studies have been described, the following observations can be made: (i) Morphometric data of E. acervulina oocysts in the present study is larger than those described by Kucera and Reznický (1991) and Khursheed et al (2022). (ii) The oocyst shape was ovoid except for those described by Williams et al (2009) and Nguyen et al (2021) which mentioned the polymorphic nature of the oocysts, which can be sub‐spherical and/or ellipsoidal, respectively.…”

Section: Discussionmentioning

confidence: 95%

“…Limitations of microscopy‐led diagnoses, which include the need for specific parasitological competence and low sample throughput, are yet to be outweighed by the difficulties associated with retrieving genomic DNA from environmental Eimeria samples (Allen & Fetterer, 2002; Vrba et al, 2010). Several techniques based on the polymerase chain reaction (PCR) have been developed to fingerprint avian Eimeria species via the amplification of small subunit ribosomal RNA ( 18S rRNA ) (Stucki et al, 1993; Tsuji et al, 1999), internal transcribed spacer ( ITS )‐1 (Clark et al, 2016; Güven et al, 2013; Hamidinejat et al, 2010; Haug et al, 2008; Khaier et al, 2015; Lew et al, 2003; Nguyen et al, 2021; Sultan et al, 2022; You, 2014), ITS ‐2 (Clark et al, 2016; Gasser et al, 2001; Jenkins et al, 2006; Lien et al, 2007; Meireles et al, 2004), mitochondrial cytochrome c oxidase 1 ( COI ) (Ogedengbe et al, 2011) and sequence characterised amplified region (SCAR) markers (Fernandez et al, 2003; Gadelhaq et al, 2015; Loo et al, 2019; Olufemi et al, 2020; Rao et al, 2012; Siddiki et al, 2014; Taha et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Eimeria acervulina (Apicomplexa: Eimeriidae) infecting the broiler chicken Gallus gallus domesticus through morphology and molecular analyses

Mohammed,

Al‐Shaebi,

Al‐Quraishy

et al. 2023

Animal Physiology Nutrition

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Coccidiosis is an intestinal protozoan disease that affects the poultry industry worldwide. The severity of this disease varies depending on the identity of the infectious agents. Therefore, this study was carried out to identify the Eimeria species that affect broiler chickens, Gallus gallus domesticus, through morphological and molecular phylogenetic analyses. Twenty‐five faecal samples were collected from the broiler chickens in a commercial poultry farm in Riyadh (Saudi Arabia). Using the floatation technique, faeces were examined microscopically for the Eimeria occurrence. Identification of Eimeria species was performed based on morphological criteria and molecular tools (DNA amplification for the partial small subunit ribosomal RNA (18S rRNA), internal transcribed spacer (ITS)‐1, and mitochondrial cytochrome c oxidase I (COI) genes. In this study, 32% (8 out of 25) of collected samples were found to be positive for coccidiosis. After sporulation in potassium dichromate (K2Cr2O7), the sporulated oocysts were observed as ovoid and measured 18.37–23.19 µm (19.87) long and 15.07–18.67 µm (16.46) wide, with the anterior location of a polar granule and absence of micropyle. These Eimeria oocysts were assumed to size and shape characteristics of Eimeria acervulina. Molecular analysis was conducted on the sequences of the polymerase chain reaction products from the three genes studied (18S rRNA, ITS‐1, and COI). At the three genes, results showed that the resultant sequences clustered with E. acervulina from different regions confirming morphological description. This study highlighted the importance of molecular techniques to detect avian Eimeria species more than the traditional morphology‐based tool to optimise the appropriate anticoccidial strategies for long‐term control in the studied area.

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Section: Discussionsupporting

confidence: 88%