Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean

Brumer, Bruna Bley; Lopes-Caitar, Valéria Stefania; Chicowski, Aline S.; Beloti, Jessica D.; Castanho, Fernanda Machado; Silva, Danielle C. Gregório da; Carvalho, Sandremir de; Lopes, Ivani O. Negrão; Soares, R. M.; Seixas, C. D. S.; Abdelnoor, R. V.; Marcelino-Guimarães, Francismar Corrêa

doi:10.1007/s10658-018-1436-5

Cited by 16 publications

(28 citation statements)

References 35 publications

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“…Pioli et al (2003) have shown the existence of variability in virulence of D. aspalathi isolates in Argentina, and propose the existence of four different physiological races of D. aspalathi based on their interaction with soybean genotypes carrying different SSC resistance genes. Similarly, occurrence of at least three races of D. aspalathi based on inoculation on soybean differential set has been identified in Brazil (Brumer et al, 2018). The presence of different D. aspalathi races can be due to selection pressure caused by deployment of specific resistant genes (Pioli et al, 1999;Pioli et al, 2003;Brumer et al, 2018).…”

Section: Discussionmentioning

confidence: 95%

“…Similarly, occurrence of at least three races of D. aspalathi based on inoculation on soybean differential set has been identified in Brazil (Brumer et al, 2018). The presence of different D. aspalathi races can be due to selection pressure caused by deployment of specific resistant genes (Pioli et al, 1999;Pioli et al, 2003;Brumer et al, 2018). Soybean genotypes carrying Rdm1, Rdm2, Rdm3, and Rdm4 conferred resistance to D. aspalathi but not to D. caulivora (Pioli et al, 2003).…”

Section: Discussionmentioning

confidence: 97%

“…Development of pycnidia in D. caulivora is controversial. While pycnidia were not present in Argentinian D. caulivora isolates (Pioli et al, 2002), picnidia with a and/or b conidia were present in isolates of Unites States (Kmetz et al, 1978;Fernández and Hanlin, 1996), Korea (Sun et al, 2012), and Brazil (Brumer et al, 2018). D. caulivora hyphae were associated with trichomes in leaves and stems, acting probably as physical adhesion sites of the hyphae.…”

Section: Discussionmentioning

confidence: 99%

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Soybean Stem Canker Caused by Diaporthe caulivora; Pathogen Diversity, Colonization Process, and Plant Defense Activation

et al. 2020

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Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1a). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora-inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a b-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Soybean Stem Canker Caused by Diaporthe caulivora; Pathogen Diversity, Colonization Process, and Plant Defense Activation

et al. 2020

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“…In another recent study, King et al [37] mapped the Considering that some Brazilian D. aspalathi isolates are able to cause disease in cv. Hutcheson and PI 398469 [28] but not in the cv. Crocket and cv.…”

Section: Genome Wide Association Study For Southern Stem Canker Diseasementioning

confidence: 88%

“…Resistance to southern stem canker is an important trait for the release of a new soybean cultivars, considering that this disease presents a high potential to cause losses of up to 100% in soybean fields [8]. In the present study, the applied method was toothpick inoculation, which has been used successfully in the evaluation of soybean materials since the first outbreaks of the disease in the late 1980s [8,13,26,28]. In our panel, 205 accessions were classified as resistant by this inoculation method, including the differential genotypes such as cv.…”

Section: Southern Stem Canker Reactions In the Gwas Panelmentioning

confidence: 99%

Association mapping of a locus that confers southern stem canker resistance in soybean and SNP marker development

Santos

Ferreira

Passianotto

et al. 2019

Preprint

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Background Southern stem canker (SSC), caused by Diaporthe aspalathi (E. Jansen, Castl. & Crous), is an important soybean disease that has been responsible for severe losses in the past. The main strategy for controlling this fungus involves the introgression of resistance genes. Thus far, five main loci have been associated with resistance to SSC. However, there is a lack of information about useful allelic variation at these loci. In this work, a genome-wide association study (GWAS) was performed to identify allelic variation associated with resistance against Diaporthe aspalathi and to provide molecular markers that will be useful in breeding programs. Results We characterized the response to SSC infection in a panel of 295 accessions from different regions of the world, including important Brazilian elite cultivars. Using a GBS approach, the panel was genotyped, and we identified marker loci associated with Diaporthe aspalathi resistance through GWAS. We identified 19 SNPs associated with southern stem canker resistance, all on chromosome 14. The peak SNP showed an extremely high degree of association ( p-value = 6.35E-27) and explained a large amount of the observed phenotypic variance (R² = 70%). This strongly suggests that a single major gene is responsible for resistance to D. aspalathi in most of the lines constituting this panel. In resequenced soybean materials, we identified other SNPs in the region identified through GWAS in the same LD block that clearly differentiate resistant and susceptible accessions. The peak SNP was selected and used to develop a cost-effective molecular marker assay, which was validated in a subset of the initial panel. In an accuracy test, this SNP assay demonstrated 98% selection efficiency. Conclusions Our results suggest relevance of this locus to SSC resistance in soybean cultivars and accessions from different countries, and the SNP marker assay developed in this study can be directly applied in MAS studies in breeding programs to select materials that are resistant against this pathogen and support its introgression

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Mapping Major Disease Resistance Genes in Soybean by Genome-Wide Association Studies

Ferreira

Marcelino-Guimarães

2022

Methods in Molecular Biology

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Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean

Cited by 16 publications

References 35 publications

Soybean Stem Canker Caused by Diaporthe caulivora; Pathogen Diversity, Colonization Process, and Plant Defense Activation

Soybean Stem Canker Caused by Diaporthe caulivora; Pathogen Diversity, Colonization Process, and Plant Defense Activation

Association mapping of a locus that confers southern stem canker resistance in soybean and SNP marker development

Mapping Major Disease Resistance Genes in Soybean by Genome-Wide Association Studies

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