Morphological and molecular datasets for Kaempferia species

Abstract

This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset.

Comparison of real time PCR and conventional PCR by identifying genomic DNA of bovine and porcine

“…Those reasons should be considered to satisfy and protect consumers. There are several approaches to detect meat species in foods, such as genomics (Wang et al 2019;Sultana et al 2020), spectroscopy (Sankar et al 2020;Cebi et al 2019), chromatography (Ekasary et al 2018;Sha et al 2018), morphology (Labrooy et al 2018;Zhang et al 2019), immunochemical (Tukiran et al 2016) and proteomics (Chis & Vodnar 2019;Wang et al 2019). The genomic method with the PCR technique has been used by to detect the species content in the product accurately.…”