Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Abstract

Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

Evaluation of certain Penicillium frequentans isolates against Cercospora leaf spot disease of sugar beet

“…c Inhibition zone between P. frequentans and C. beticola Kawano et al (1999) who noted that P. frequentans produced high levels of extracellular pectinases after 24 h of incubation in submerged culture. Moreover, many researches have shown the ability of different Penicillium species to produce some enzymes, such as P. italicum (Alana et al 1990), pectolytic molds (Fawole and Odunfa 1992), P. viridicatum RFC3 (Silva et al 2002), P. roqueforti (Pericin et al 2007), and P. expansum (Cardoso et al 2007). From these results, it is clear that there was a correlation between enzyme activity and the antagonistic ability for each isolate.…”

Characterization of cultivated fungi isolated from grape marc wastes through the use of amplified rDNA restriction analysis and sequencing

“…DQ325456-DQ325457 T. mycotoxinivorans (Molnar et al, 2004) and strain R-4272 (Hickey et al, 2009); G. loubieri (Scorzetti et al, 2002 (Wu et al, 2003) 99.7% DQ325458-DQ325459 P. italicum (AJ250548), P. commune (Buzina et al, 2003), P. expansum (Cardoso et al, 2007), P. solitum var. crustosum (Vega et al, 2006;Bakys et al, 2009) (EF634415), and P. griseoroseum (Cardoso et al, 2007) (Wu et al, 2003) and A. awamori (Nikkuni et al, 1996) 99.9% DQ325463 A. niger (Buzina et al, 2003;Haugland et al, 2004;Huitron et al, 2008), A. awamori and A. foetidus (Haugland et al, 2004) 99.1% a profiles did not consider restriction fragments under 70 bp.…”

“…crustosum (Vega et al, 2006;Bakys et al, 2009) (EF634415), and P. griseoroseum (Cardoso et al, 2007) (Wu et al, 2003) and A. awamori (Nikkuni et al, 1996) 99.9% DQ325463 A. niger (Buzina et al, 2003;Haugland et al, 2004;Huitron et al, 2008), A. awamori and A. foetidus (Haugland et al, 2004) 99.1% a profiles did not consider restriction fragments under 70 bp. several toxic metabolites, which present potent immunosuppressive, genotoxic, cytotoxic, and apoptotic effects (Latge, 2001;Boudra and Morgavi, 2005).…”

Morphological and Molecular Differentiation of Sporidiobolus johnsonii ATCC 20490 and Its Coenzyme Q10 Overproducing Mutant Strain UF16

“…Morphological variations and molecular differentiation methods like rRNA gene sequences, RAPD, AFLP or RFLP are being used to discriminate the strains of same genera or species and mutants [21,[23][24][25][26]. The objective of this study was to generate the mutant strain of S. johnsonii having significant improvement in CoQ 10 content and to differentiate it from wild type strain, based on cultural and microscopic morphology, rRNA gene sequences and physiological attributes.…”