Morphological and Molecular Effects of<i>Vibrio splendidus</i>on Hemocytes of Softshell Clams,<i>Mya arenaria</i>

Araya, Mebrahtu T.; Siah, Ahmed; Mateo, Dante R.; Markham, Frederick; McKenna, Patricia; Johnson, Gerald R.; Berthe, Franck

doi:10.2983/035.028.0403

Cited by 19 publications

(5 citation statements)

References 53 publications

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“…Among the transcripts from both top20s affected by V. splendidus exposure, several (polyubiquitin, ferritin, paramyosin and actin) were differentially expressed after bacterial challenge of Ruditapes decussatus [45]. Interestingly, actin, which is commonly used as a reference gene, was affected by V. splendidus exposure, as previously reported for R. decussatus [45] and Mya arenaria [46,47]. The transcript annotated as transferrin coded for a partial sequence sharing sequence identity with salmon (Oncorhynchus kisutch) transferrin-like protein (41.16%, P79815.1).…”

Section: Results-discussionsupporting

confidence: 76%

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In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

et al. 2020

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Cuttlefish (Sepia officinalis) haemocytes are potential sources of antimicrobial peptides (AMPs). To study the immune response to Vibrio splendidus and identify new AMPs, an original approach was developed based on a differential transcriptomic study and an in-depth in silico analysis using multiple tools. Two de novo transcriptomes were retrieved from cuttlefish haemocytes following challenge by V. splendidus or not. A first analysis of the annotated transcripts revealed the presence of Toll/NF-κB pathway members, including newly identified factors such as So-TLR-h, So-IKK-h and So-Rel/NF-κB-h. Out of the eight Toll/NF-κB pathway members, seven were found up-regulated following V. splendidus challenge. Besides, immune factors involved in the immune response were also identified and up-regulated. However, no AMP was identified based on annotation or conserved pattern searches. We therefore performed an in-depth in silico analysis of unannotated transcripts based on differential expression and sequence characteristics, using several tools available like PepTraq, a homemade software program. Finally, five AMP candidates were synthesized. Among them, NF19, AV19 and GK28 displayed antibacterial activity against Gram-negative bacteria. Each peptide had a different spectrum of activity, notably against Vibrio species. GK28—the most active peptide—was not haemolytic, whereas NF19 and AV19 were haemolytic at concentrations between 50 and 100 µM, 5 to 10 times higher than their minimum inhibitory concentration.

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Section: Results-discussionsupporting

confidence: 76%

“…splendidus exposure, as previously reported for R . decussatus [ 45 ] and Mya arenaria [ 46 , 47 ]. The transcript annotated as transferrin coded for a partial sequence sharing sequence identity with salmon ( Oncorhynchus kisutch ) transferrin-like protein (41.16%, P79815.1).…”

Section: Results—discussionmentioning

confidence: 99%

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

et al. 2020

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“…For example, adhesion and phagocytic activity of pacific oyster hemocytes were altered in contact with V. aestuarianus M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 01/32 ECPs or V. tubiashii 07/118 T2 ECPs (Labreuche et al, 2006;Mersni-Achour et al, 2014) in a manner that was dependent on ECPs doses (Labreuche et al, 2006). The same hemocyte responses were reported for Mytilus edulis and Mya arenaria hemocytes challenged with the oyster pathogen V. splendidus LGP32 (Araya et al, 2009;Tanguy et al, 2013).…”

Section: Accepted Manuscriptmentioning

confidence: 59%

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities

Cheikh

Travers

Morga

et al. 2016

Fish & Shellfish Immunology

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Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010-2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions. Highlights ► Isolation of a Vibrio strain pathogenic to the blue mussel. ► Two differentially virulent Vibrio strains were GFP-labeled. ► The virulent strain altered hemocyte motility and quenched ROS production. ► ECPs from the virulent strain inhibited bead phagocytosis and ROS production. ► The virulent strain was rapidly internalized in hemocytes.

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“…In addition, hyalinocytes may be nonphagocytic (Carballal et al, 1997b). Generation of reactive nitrogen intermediates (RNIs) such as nitric oxide is an evolutionarily sustained strategy of phagocytosis, which is employed by bivalve haemocytes to combat pathogenic attacks (Araya et al, 2009; Chakraborty et al, 2009; Park et al, 2012). Most studies in the field of molluscan immunology have only focussed on the phagocytic activity of bivalves, but relatively little information is available on phagocytic activity in different seasons and natural environmental changes, which are needed to understand seasonal impacts on the immune system of bivalves.…”

Section: Introductionmentioning

confidence: 99%

Characterization of mussel ( Perna canaliculus ) haemocytes and their phagocytic activity across seasons

Muznebin

Webb

Mérien

2022

Aquaculture Research

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Perna canaliculus is endemic to New Zealand and is the top shellfish export product.However, the growth of this industry is being adversely affected by summer mortality events. To assess these health threats, differential haemocyte counts (DHC) were performed on haemolymph smears stained with Giemsa, and in vitro phagocytosis activity assays were applied in P. canaliculus for several months covering three seasons (winter, spring and summer). A new optimized classification scheme was developed for P. canaliculus haemocytes where 55% eosinophilic and 27.2% basophilic granulocytes were identified. Eosinophilic granulocytes were classified as dense, semi and small semi, and these new categories were reported herein for the first time in this species.A new haemocyte type (mixed granulocytes), which contains both acidophilic and basophilic granules, is proposed for P. canaliculus. The phagocytosis percentages were significantly affected by incubation time, indicating an increase in zymosan particle uptake from 18.42 ± 1.7% after 30 min, 32.08 ± 3.1% after 60 min and 44.74 ± 3.5% (maximum) after 120 min of incubation. The lowest phagocytosis was observed in the winter season and the highest phagocytosis in summer. This study findings provide a better understanding of the immune function of P. canaliculus haemocytes and serve as a reference for further investigations on the impacts of seasonal variations on haemocyte activity and phagocytosis.

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Morphological and Molecular Effects ofVibrio splendiduson Hemocytes of Softshell Clams,Mya arenaria

Cited by 19 publications

References 53 publications

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes

First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities

Characterization of mussel ( Perna canaliculus ) haemocytes and their phagocytic activity across seasons

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