Morphological and molecular identification of Phlebia wuliangshanensis sp. nov. in China

Abstract

A new white-rot fungus, Phlebia wuliangshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with a smooth to tuberculate hymenial surface, a monomitic hyphal system with thin- to thick-walled generative hyphae bearing simple septa, presence of cystidia, and narrow ellipsoid to ellipsoid basidiospores (5–6 × 3–3.7 μm). Our phylogenetic analyses of ITS and LSU nrRNA sequences performed with maximum likelihood, maximum parsimony, and Bayesian inference methods support P. wuliangshanensis within a phlebioid clade in Meruliaceae (Polyporales). ITS+nLSU sequence analyses of additional Phlebia taxa strongly support P. wuliangshanensis within a monophyletic lineage grouped with P. chrysocreas and P. uda .

Multi-Gene Phylogeny and Taxonomy of the Wood-Rotting Fungal Genus Phlebia sensu lato (Polyporales, Basidiomycota)

“…Across all species of Phlebia s.l., the smooth, tuberculata, phlebioid, merulioid, odontioid–hydnoid, and poroid hymenophores are varied, such as the phlebioid species ( Phlebia radiata ), merulioid species ( Merulius tremellosus ), hydnoid species ( Mycoacia fuscoatra ), poroid species ( Ceriporiopsoides guidella ), smooth species ( Luteochaete subglobosa ), and the tuberculate species ( Phlebicolorata brevispora ). Traditionally, the hymenophore configurations of morphological study for this group play a core role, but the macromorphology of fruiting bodies and hymenophore construction did not reflect monophyletic features for this group in the present study ( Figure 1 ), which is generally consistent with previous phylogenetic studies [ 32 , 33 , 34 , 38 , 43 , 44 ]. The evolution mechanism for morphology and phylogeny is still confusing mycologists.…”

“…is provided by employing seven gene phylogenetic analyses at the intergeneric level ( Figure 1 ), which discusses the circumscription and phylogenetic relationships of genera in Phlebia s.l. The tree topologies are generally consistent with previous phylogenetic studies using ribosomal RNA genes (rDNA) alone or in combination with the protein-coding genes [ 28 , 31 , 32 , 33 , 34 , 38 ]. However, some minor differences still exist due to the different scales of sampling and lower supports for several topologies in this group.…”

“…Phlebia s.l. species are an extensively studied group, distributed worldwide [ 7 , 8 , 13 , 14 , 15 , 16 , 17 , 27 , 28 , 29 , 30 , 32 , 33 , 34 , 106 , 107 ] and mainly found on hardwood, although a few species grow on coniferous wood [ 7 , 106 ]. Many species of Phlebia s.l.…”

“…(Meruliaceae, Polyporales), erected by Fries [ 8 ] with P. radiata Fr. as the type species, is a large, cosmopolitan genus characterized by the effused or partly pileate basidiomata with a subceraceous to subgelatinous texture when fresh, membranaceous to coriaceous when dry, hymenophore smooth, tuberculate, phlebioid, odontioid, merulioid or poroid, a monomitic (rarely dimitic) hyphal structure generally with clamped hyphae, the embedded generative hyphae very difficult to observe, narrowly clavate basidia, and colorless, thin-walled, smooth, allantoid to ellipsoid basidiospores, which are acyanophilous, in-amyloid and non-dextrinoid [ 7 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Currently, about 100 species have been accepted in the genus worldwide [ 34 , 35 , 36 ].…”

“…Huang et al have run a phylogenetic analysis, which showed that Phlebia species clustered into phlebioid clade with three new Phlebia species viz. P. fuscotuberculata , P. tomentopileata, and P. tongxiniana from southern China [ 32 ].…”