Morphological and molecular identification of Fusarium spp. isolated from maize kernels in Java and Lombok, Indonesia

Widiastuti, Ani; Karlina, Monica Lucky; Dhanti, Kurnia Ritma; Chinta, Yufita Dwi; Joko, Tri; Suryanti, Suryanti; Wibowo, Arif

doi:10.13057/biodiv/d210650

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…The same species were recorded in this study. Some Fusarium species, such as F. verticillioides, F. proliferatum, F. oxysporum, F. bullatum and F. thapsinum were recovered from maize ears collected from 11 different geographic regions in Iran during 2004 and 2005 [30] , while in Indonesia, a research study found four species of mycotoxigenic Fusarium species isolated from maize based on molecular identification, which were Fusarium verticillioides, F. proliferatum, F. graminearum and F. asiaticum [41] . This study found that the occurrence of F. verticillioides in corn fields in the Kurdistan region was most commonly compared to other isolated species; this finding is consistent with those studies carried out in other countries such as Iran, India, Indonesia [37,36,30] , and not agree with the results reported by research carried out in Egypt, which F. oxysporum is the most common species [34] .…”

Section: Morphological Identification Of Fusarium Speciesmentioning

confidence: 99%

Molecular Detection of Fusarium species infected Corn in Kurdistan region-Iraq

Kakakhan,

Shekhany

2023

PJBAS

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Corn or Maize product is regarded as one of the essential products in the world and stands third product after the rice and wheat crops. Different fungal pathogens attack corn plants; one of them is ear rot, brought on by Fusarium species and whose occurrence is primarily influenced by environmental factors. In order to isolate and identify Fusarium species from corn plants and their prevalence, 50 samples of corn were collected during September, October, and November of 2021 from 30 corn fields in 14 regions of different places in the Kurdistan region of Iraq. From all samples, 39 isolates of Fusarium were detected and based on morphological characteristics, six other species of Fusarium were identified, namely F. verticillioides (33.34%), F. proliferatum (25.64%), F. oxysporum (12.82%), F. incarnatum and F. equiseti (10.25% each), then F. fujikuroi (7.7%). The most prevalent species was F. verticillioides which was isolated from seven corn fields and significantly higher than all other isolated species. All Fusarium isolates were also molecularly identified depending on amplifying the internal transcribed spacer (ITS) universal region using forward ITS1 and reverse ITS4 primers and indicated DNA fragments ranged from 550 to 570 bp. The PCR fragments of the amplified ITS region were sequenced, aligned and registered in NCBI GeneBank with specified accession numbers. The phylogenetic tree and all analyzes were performed using the MEGA program version 11.0.13. The current study concluded that the corn fields in the Kurdistan region are infected with different Fusarium species, and the most common species is F. verticillioides. As well as the Fusarium species in the Kurdistan region have close evolutionary history to the same species in other countries. Thus, the study recommends more research to investigate the occurrence of toxigenic Fusarium species associated with cereal grains in the region.

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Section: Morphological Identification Of Fusarium Speciesmentioning

confidence: 99%

Molecular Detection of Fusarium species infected Corn in Kurdistan region-Iraq

Kakakhan,

Shekhany

2023

PJBAS

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rep-PCR analysis of Fusarium proliferatum causing sheath rot disease and its relationship to light, pH, temperature and rice varieties

Pramunadipta

Widiastuti

Wibowo

et al. 2022

Archives of Phytopathology and Plant Protection

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Exploration of the indigenous rhizosphere fungi as corncob waste decomposers and its potential as biological agent Fusarium graminearum and increase corn growth

Nurbailis

Liswarni

Yanti

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Corncob waste can be used as compost; corncob compost that microbial decomposers have composted positively affects soil and plants. The objectives of this study are 1). It was obtaining the type of fungi from the corn rhizosphere that acts as a decomposer of corncob waste and 2) knowing the potential of corncob waste compost in suppressing F. graminearum, which causes stem rot on corn and increasing plant growth. The designs used for the two steps of the study were: Randomized Block Designs with ten treatments and four replication. The first step is using the rhizosphere fungi isolates for composting corncob waste. The second step is testing corncob composted by ten different types of rhizosphere fungi to suppress corn stem rot caused by F. graminearum. Parameters observed in the first step were: the number of conidia and the number of propagules of rhizosphere fungi in corncobs compost. The third step was: Incubation period, disease incidence, disease severity, number of dead plants, and plant growth. The result of the research indicated that the corn rhizosphere obtained 15 isolates of fungi that varied the color, shape of the colony, and growth rate. The highest number of propagules was found in compost decomposed by rhizosphere fungi RF4, while the number of conidia classified as high was found in RF4, Rf, and RF6 compost. All corncob compost decomposed by rhizosphere fungi was able to suppress stem rot disease caused by F. graminearum.

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Morphological and molecular identification of Fusarium spp. isolated from maize kernels in Java and Lombok, Indonesia

Cited by 3 publications

References 14 publications

Molecular Detection of Fusarium species infected Corn in Kurdistan region-Iraq

Molecular Detection of Fusarium species infected Corn in Kurdistan region-Iraq

rep-PCR analysis of Fusarium proliferatum causing sheath rot disease and its relationship to light, pH, temperature and rice varieties

Exploration of the indigenous rhizosphere fungi as corncob waste decomposers and its potential as biological agent Fusarium graminearum and increase corn growth

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