Morphological and molecular identification of acridid grasshoppers (Acrididae: Orthoptera) from Poonch division, Azad Jammu Kashmir, Pakistan

Nazir, Naila; Mehmood, Khalid; Ashfaq, Muhammad; Rahim, Junaid

doi:10.11609/jott.o3507.5544-52

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The short-horned species and long horned-species were grouped in two different clades. Our findings support the results of Hawlitschek et al (2017) and Nazir et al (2014).…”

Section: Interspecific Genetic Distancesupporting

confidence: 92%

Molecular Characterization and Identification of Grasshopper Pests of Rice Inferred From Mitochondrial COI Gene Sequences

Das

Islam

Aslam

2022

Bangladesh J. Zool.

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Oryza sativa (L.) is one of the world’s most important crop providing staple food for nearly half of the global population. Rice is subjected to considerable damage by a number of grasshoppers. The accurate identification of pest species is a crucial step for the appropriate application of control measures. Molecular characterization and DNA barcoding of grasshopper pests of rice was performed in the present research work. Six species of grasshopper pests were identified from different paddy fields using COI gene sequences. Among the sequences the A+T content (64.2%) was higher than the G+C (35.8%) content. The interspecific genetic distance ranged between 13 and 34%. The haplotype analysis of Conocephalus exemptus showed highest (110) mutated sites among the analyzed species. Both the Maximum likelihood and Neighbor-joining phylogenetic tree construction showed two distinct clades of short-horned and long-horned grasshoppers. C. exemptus was found in another monophyletic group. Our results demonstrate that DNA barcoding is likely to be a powerful tool for identifying and understanding grasshopper evolution. Bangladesh J. Zool. 50 (2): 217-229, 2022

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“…The short-horned species and long horned-species were grouped in two different clades. Our findings support the results of Hawlitschek et al (2017) and Nazir et al (2014).…”

Section: Interspecific Genetic Distancesupporting

confidence: 92%

Molecular Characterization and Identification of Grasshopper Pests of Rice Inferred From Mitochondrial COI Gene Sequences

Das

Islam

Aslam

2022

Bangladesh J. Zool.

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“…In brief, once the barcode data was on BOLD, each record went through a taxonomic assignment and verification workflow. Earlier studies ( Ashfaq et al, 2013 ; Nazir et al, 2014 ; Iftikhar et al, 2016b ; Akhtar et al, 2018 ; Naseem et al, 2019 ) on five taxa (antlions, aphids, butterflies, grasshoppers, thrips) coupled analysis of barcode results with detailed morphological study by taxonomic specialists. All sequences meeting the quality criteria were either assigned to an existing BIN or founded a new one ( Ratnasingham & Hebert, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

A DNA barcode survey of insect biodiversity in Pakistan

Ashfaq

Khan

Rasool

et al. 2022

PeerJ

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Although Pakistan has rich biodiversity, many groups are poorly known, particularly insects. To address this gap, we employed DNA barcoding to survey its insect diversity. Specimens obtained through diverse collecting methods at 1,858 sites across Pakistan from 2010–2019 were examined for sequence variation in the 658 bp barcode region of the cytochrome c oxidase 1 (COI) gene. Sequences from nearly 49,000 specimens were assigned to 6,590 Barcode Index Numbers (BINs), a proxy for species, and most (88%) also possessed a representative image on the Barcode of Life Data System (BOLD). By coupling morphological inspections with barcode matches on BOLD, every BIN was assigned to an order (19) and most (99.8%) were placed to a family (362). However, just 40% of the BINs were assigned to a genus (1,375) and 21% to a species (1,364). Five orders (Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera) accounted for 92% of the specimens and BINs. More than half of the BINs (59%) are so far only known from Pakistan, but others have also been reported from Bangladesh (13%), India (12%), and China (8%). Representing the first DNA barcode survey of the insect fauna in any South Asian country, this study provides the foundation for a complete inventory of the insect fauna in Pakistan while also contributing to the global DNA barcode reference library.

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Diversity, species richness and distribution of grasshoppers (Orthoptera: Acrididae) in Khairpur Sindh Pakistan

Panhwar,

Ahmed,

Mehmood

et al. 2024

Int J Trop Insect Sci

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Morphological and molecular identification of acridid grasshoppers (Acrididae: Orthoptera) from Poonch division, Azad Jammu Kashmir, Pakistan

Cited by 4 publications

References 31 publications

Molecular Characterization and Identification of Grasshopper Pests of Rice Inferred From Mitochondrial COI Gene Sequences

Molecular Characterization and Identification of Grasshopper Pests of Rice Inferred From Mitochondrial COI Gene Sequences

A DNA barcode survey of insect biodiversity in Pakistan

Diversity, species richness and distribution of grasshoppers (Orthoptera: Acrididae) in Khairpur Sindh Pakistan

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