Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

Bich, Gustavo Ángel; Castrillo, María Lorena; Kramer, Fernando Luis; Villalba, Laura Lidia; Zapata, Pedro Darío

doi:10.1590/2179-8087-floram-2018-0086

Cited by 25 publications

(13 citation statements)

References 29 publications

(34 reference statements)

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“…MT448732.1), meaning that the isolates had a high phylogenetic relationship and were in the same species. Ribosomal DNA sequences were used to determine the phylogenetic relationships of organisms to taxa species (Bich et al 2021). Henry et al (Henry et al 2000) stated that the similarity value of 99% shows that the isolates are the same species.…”

Section: Discussionmentioning

confidence: 99%

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

et al. 2022

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Fungi from South Sumatra (Indonesia) were identified morphologically and molecularly, and their pathogenicity to egg, larvae, and adult Aedes aegypti was evaluated. The fungal isolates used for bioassay were 11 isolates from this study and 4 isolates from the laboratory collection. Fifteen isolates of five fungal species (Metarhizium anisopliae, Penicillium citrinum, Talaromyces diversus, Beauveria bassiana, and Purpureocillium lilacinum) from South Sumatra, Indonesia, were pathogenic to the egg, larvae, and adult of Ae. aegypti. Egg mortality caused by M. anisopliae isolate MSwTp3 was the highest (38.31%). A novel finding of this study was that the eggs exposed to the fungus not only killed the eggs but could continue to kill the emerging larvae, pupae, and adults. The five fungal species induced larval mortality between 52.22−94.44% and adult mortality between 50.00−92.22%. Fungal strains belonging to M. anisopliae, P. citrinum, T. diversus, and B. bassiana from South Sumatra seem to possess remarkable ovicidal, larvicidal and adulticidal activity against Ae. aegypti. M. anisopliae, P. citrinum, T. diversus, and B. bassiana had the potential as entomopathogens to be developed into ovicides, larvicides, and adulticides for controlling Ae. aegypti.

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Section: Discussionmentioning

confidence: 99%

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

et al. 2022

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“…No contamination was observed after sterilization of the stem and leaves, however, in a few cases primary root sections showed random microbial outgrowth. The identification of endophytic fungi growing from edges was done by colony morphology and conidial structures of M. brunneum ( Humber, 1997 ; Bich et al, 2021 ). The Metarhizium outgrowth from plant tissue pieces was recorded as a value of one, while no outgrowth was recorded as a value of zero.…”

Section: Methodsmentioning

confidence: 99%

Effects of tomato inoculation with the entomopathogenic fungus Metarhizium brunneum on spider mite resistance and the rhizosphere microbial community

et al. 2023

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Entomopathogenic fungi have been well exploited as biocontrol agents that can kill insects through direct contact. However, recent research has shown that they can also play an important role as plant endophytes, stimulating plant growth, and indirectly suppressing pest populations. In this study, we examined the indirect, plant-mediated, effects of a strain of entomopathogenic fungus, Metarhizium brunneum on plant growth and population growth of two-spotted spider mites (Tetranychus urticae) in tomato, using different inoculation methods (seed treatment, soil drenching and a combination of both). Furthermore, we investigated changes in tomato leaf metabolites (sugars and phenolics), and rhizosphere microbial communities in response to M. brunneum inoculation and spider mite feeding. A significant reduction in spider mite population growth was observed in response to M. brunneum inoculation. The reduction was strongest when the inoculum was supplied both as seed treatment and soil drench. This combination treatment also yielded the highest shoot and root biomass in both spider mite-infested and non-infested plants, while spider mite infestation increased shoot but reduced root biomass. Fungal treatments did not consistently affect leaf chlorogenic acid and rutin concentrations, but M. brunneum inoculation via a combination of seed treatment and soil drenching reinforced chlorogenic acid (CGA) induction in response to spider mites and under these conditions the strongest spider mite resistance was observed. However, it is unclear whether the M. brunneum-induced increase in CGA contributed to the observed spider mite resistance, as no general association between CGA levels and spider mite resistance was observed. Spider mite infestation resulted in up to two-fold increase in leaf sucrose concentrations and a three to five-fold increase in glucose and fructose concentrations, but these concentrations were not affected by fungal inoculation. Metarhizium, especially when applied as soil drench, impacted the fungal community composition but not the bacterial community composition which was only affected by the presence of spider mites. Our results suggest that in addition to directly killing spider mites, M. brunneum can indirectly suppress spider mite populations on tomato, although the underlying mechanism has not yet been resolved, and can also affect the composition of the soil microbial community.

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“…The sequence of ITS region of 18S rDNA of Metarhizium PB-75 was 1,218 base pairs in length, with a molecular weight at 367,667.00 for single-stranded and 739,779.00 Daltons for doublestranded, with slightly high AT content (53.28%). Ribosomal DNA sequences are commonly used to identify and determine the phylogenetic relationships of organisms to taxa species [62]. The organisms which have a similarity value of 100% can be stated as the same strain and a similarity value of 99% is stated as the same species, while the similarity value of 89-99% belongs to the same genus [63].…”

Section: Similarity Of Its Region Of 18s Rdna Of Metarhizium Pb-75 Co...mentioning

confidence: 99%

Occurrence of Entomopathogenic Fungi from Natural Ecosystems in Phetchabun, Thailand and Their Virulence Against Brown Planthopper

Bunsak

Homhaul

Pongprasert

et al. 2022

Curr. Appl. Sci. Technol.

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Brown planthopper (BPH), Nilaparvata lugens Stål, is a serious insect pest of rice. The invasion of BPH causes heavy losses in rice production, both quality and quantity. The objective of this research was to search for Metarhizium spp., a genus of entomopathogenic fungi in the Clavicipitaceae family, from forest soil in three districts of Phetchabun Province, Thailand: Khao Kho, Lom Kao and Nam Nao to control BPH. Metarhizium spp. fungi were isolated by soil dilution plate technique and determined their efficacy using conidia suspension at the concentration of 1X108 conidia/mL on BPH at the 2-3th instar nymph fed on seedlings of susceptible rice cultivars, Taichung Native 1 (TN1). The most effective Metarhizium sp. isolate was identified using the ITS region of 18S rDNA sequencing, Basic Local Alignment Search Tool (BLAST) and evolutionary history. The result showed that 126 isolates of Metarhizium spp. were found and coded as PB-01 to PB-126. All isolates infected BPH nymph with 26.7 to 100% mortality within 6 days after contacting conidia suspension. The isolate of PB-75 showed the highest efficacy (100% BPH mortality) with the lethal times of 50% mortality (LT50) within 2.9 days. The species identification showed that the DNA sequence of Metarhizium sp. isolate, PB-75, was 98.6% similar to M. anisopliae Genbank ID JQ889704.1. Evolutionary history based on phylogenetic analysis using neighbor-joining and maximum parsimony methods confirmed that PB-75 formed the same phylogenetic clade with M. anisopliae Genbank ID JQ889704.1 and M. anisopliae var. anisopliae.

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Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

Cited by 25 publications

References 29 publications

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

Effects of tomato inoculation with the entomopathogenic fungus Metarhizium brunneum on spider mite resistance and the rhizosphere microbial community

Occurrence of Entomopathogenic Fungi from Natural Ecosystems in Phetchabun, Thailand and Their Virulence Against Brown Planthopper

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