Morphological and morphometric characterization of agoutis' peripheral blood cells (Dasyprocta prymnolopha, wagler, 1831) raised in captivity

Abstract

Thirty adult agoutis (Dasyprocta primnolopha) from the Nucleus of Study and Preservation of Wild Animals at the Federal University of Piauí were used. Blood scrubs of these animals were colored by the Leishman method and analyzed in light microscopy. The cells had been measured using programs that analyze images (Leica QWin - Image Processing and Analysis Software). Mature erythrocytes, basophil reticulocytes, lymphocytes, eosinophils, neutrophils, monocytes, and thrombocytes were identified. Agoutis' erythrocytes presented elliptical form, without nucleus with an average diameter of 5.64 micromeres ± 0.38. The lymphocytes are spherical cells with scarce cytoplasm, dense and with a very centralized rounded nucleus measuring an average diameter of 13.20 micromeres ± 0.35. The monocytes are slightly basophilic, with a spherical nucleus, central constriction, and an average diameter of 20.59 micromeres ± 0.32. The neutrophils are spherical, with a polymorphic lobulated nucleus, with an average diameter of 11.2 micromeres ± 0.20. The eosinophils are spherical with lobulated nucleus and with an average diameter of 14.25 micromeres ± 0.36. Only five basophils were observed, with abundance of cytoplasmic granules with 9.8 micrometers of diameter ± 0.30. Thrombocytopenic pleomorphism was frequent. There were similarities in the cellular constituents in peripheral blood of agoutis and of other rodents and humans. The cellular types from the peripheral blood, the morphology, and morphometry of the blood's cells did not vary according to sex.

Hematological and serum biochemistry profiles of collared peccaries (Pecari tajacu Linnaeus, 1758) from the semi-arid brazilian northeast

“…Values are also similar to those reported for rabbits (POLJIČAK-MILAS et al, 2009) and hoary foxes (SILVA; LIMA; SANCHEZ, 2004). Averaging 10.34 μm, lymphocytes did not differ significantly between males and females, similar to other studies on the same species ( ALMEIDA et al, 2011), but higher than values reported for wood rats (9.67 μm) (SILVA; BOLETTI; FERRO, 2003) and hoary foxes (8.91μm) (SILVA; LIMA; SANCHEZ, 2004), while agouti lymphocytes are reported as considerably larger than peccary lymphocytes, at 13.2 μm (CONDE JÚNIOR et al, 2012).…”

“…Lymphocytes were spherical in shape, with scant and basophilic cytoplasm, a round and central nucleus, loose chromatin and small areas of condensed chromatin, consistent with descriptions for agoutis (CONDE JÚNIOR et al, 2012) and capybaras (VIEIRA et al, 2021), except for intracytoplasmic glycoprotein material inclusions (Kurloff corpuscles), which were not detected in peccaries. Values are also similar to those reported for rabbits (POLJIČAK-MILAS et al, 2009) and hoary foxes (SILVA; LIMA; SANCHEZ, 2004).…”

“…As for neutrophils, average diameters were 15.42 μm for males and 15.58 μm for females with no significant difference between sex (p≤ 0.8). These values are higher than those reported for ground rats (Calomys callosus) (SILVA; BOLETTI; FERRO, 2003), agoutis (CONDE JÚNIOR et al, 2012) and other peccaries (ALMEIDA et al, 2011).…”

“…The average annual temperature is of about 27.4°C, but can reach 36°C at certain times of the day, with a relative humidity of around 70%. (COSTA SARAIVA;VALE;ZANELLA, 2017).…”

“…Hematological and serum biochemistry assessments, in addition to being useful in disease diagnoses, are paramount in evaluating physiological states, allowing for the monitoring of hematopoietic tissue responses, nutritional status, stress conditions, parasite exposure, trauma and habitat quality, of both wildlife and captive animals (CROOKS et al, 2000;GUERCI, 1985;LOCHMILLER;GRANT, 1984; SEAL; MECH; VAN BALLENBERGUE,1975). The determination of hematological patterns in wild animals is crucial for the establishment of normal values, and essential in interpreting changes observed following procedures under different clinical contexts (MADELLA et al, 2006).…”