Morphological and phylogenetic study of five species of Astrocystis and Collodiscula on bamboo

Abstract

Five taxa that morphologically resemble Astrocystis and Collodiscula were collected on bamboo culms from Guizhou, Hainan and Yunnan provinces in China. Based on morphology and molecular analyses (combined ITS, RPB2, β-tubulin and α-actin genes), these species were identified as Astrocystis cocoes (a new record to China), A. bambusicola, Collodiscula japonica and two new species (A. multiloculata and C. lancangjiangensis). Astrocystis multiloculata can be distinguished from other Astrocystis species by its superficial stromata containing many perithecia and larger ascospores (19–25 × 7–11 μm). Collodiscula lancangjiangensis has larger ascospores (26–36.5 × 5–7.5 μm) than those of the most similar species C. fangjingshanensis (19–25.5 × 4.5–6 μm). It also has larger, 3-septate ascospores than C. japonica (1-septate, 19–26 × 4.5–5.5 μm).

Two new species and a new record of Astrocystis from Southwestern China

“…It is worth noting that Kretzschmariella culmorum (JDR 88) grouped with A. multiloculata and A. tessellati with high statistical support (100 % ML, 1 BYPP, Figure 1), and this result agrees with previous studies (Johnston et al 2016, Wu et al 2021. Morphologically, three species with many perithecia in superficial stromata, while, Kretzschmariella culmorum is mainly in its possession of a cellular appendage in some mature ascospores (Fournier et al 2018).…”

“…The ITS, rpb2, tub2 and α-actin data matrices were combined for subsequent phylogenetic analyses. The sequences (Table 2) used for analyses were retrieved from NCBI-BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) results and some relevant studies (Li et al 2015a, b, Wendt et al 2018, Xie et al 2020, Wu et al 2021. After exclusion of ambiguously aligned regions and long gaps, the final combined data matrix contained 3231 characters (1-444 nucleotides of ITS, 445-1466 nucleotides of rpb2, 1467-2348 nucleotides of tub2 and 2349-3231 nucleotides of α-actin).…”

“…Morphologically, Astrocystis resembles Collodiscula Hino & Katumoto which has superficial carbonaceous stromata, and most of them were discovered from bamboo; meanwhile, the multi-gene analysis indicated that both genera have closer affinities in Xylariaceae (Li et al 2015a, b, Hyde et al 2017, Wu et al 2021. But, species of Collodiscula have larger, one to multi-septate ascospores with a germ slit (except C. leigongshanensis Q.R.…”

“…But, species of Collodiscula have larger, one to multi-septate ascospores with a germ slit (except C. leigongshanensis Q.R. Li et al) (Xie et al 2020) (Taylor and Hyde 2003, Hyde et al 2019, Wu et al 2021.…”

Bambusicolous Fungi in Pleosporales: Introducing Four Novel Taxa and a New Habitat Record for Anastomitrabeculia didymospora

“…Noteworthily, major studies of bambusicolous ascomycetes with modern taxonomic treatments have been carried out by Dai et al [ 3 ], Liu et al [ 31 , 32 ], Phookamsak et al [ 39 ], and Tanaka et al [ 27 ]. Up to now, more than 175 bambusicolous ascomycetes have been described based on morphological and phylogenetic evidence [ 13 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. However, there have been few thorough studies into the phylogeny-based taxonomy of bambusicolous fungi preceding 2002 [ 11 ], causing more than 80% of bambusicolous fungi to lack molecular data that could clarify their phylogenetic placement.…”