Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes

Jacquot, Adrien; Francius, Grégory; Razafitianamaharavo, Angélina; Dehghani, Fariba; Tamayol, Ali; Linder, Michel; Arab‐Tehrany, Elmira

doi:10.1371/journal.pone.0107435

Cited by 24 publications

(20 citation statements)

References 31 publications

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“…The full analysis of electron density profiles of the liposome membrane evidenced a bilayer thickness of about 4.2 nm ( Figure 5 ). This is strongly consistent with previous observations by atomic force microscopy (AFM) for a DOPC bilayer [ 21 ] but differs from the repeating observed distance of 6.0 nm. It means that the thickness of the water layer between two phospholipidic bilayers is approximately 2 nm in the experimental conditions.…”

Section: Resultssupporting

confidence: 92%

“…Liposome size distribution and ζ-potential were analyzed by dynamic light scattering (DLS) (Malvern Instruments Ltd., Worcestershire city, UK) with a laser emitting at 633 nm as described before [ 21 ]. Liposomes scattering intensity was measured at 25 °C and a scattering angle of 173°.…”

Section: Methodsmentioning

confidence: 99%

“…Liposomes were prepared as previously described [ 21 ]. However, the buffer used contained cations to permit liposomes ruptures and fusion on mica supports (buffer solution with 100 mM NaCl, 10 mM Tris, 3 mM CaCl 2 , adjusted to pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

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Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Arab‐Tehrany

Elkhoury

Francius

et al. 2020

IJMS

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Curcumin is a hydrophobic drug gaining growing attention because of its high availability, its innocuity, and its anticancer, antitumoral, and antioxidative activity. However, its poor ‎‎bioavailability in the human body, caused by its low aqueous solubility and fast degradation, ‎‎presents a big hurdle for its oral administration. Here, we used nano-vesicles made of ‎‎phospholipids to carry and protect curcumin in its membrane. Various curcumin amounts were ‎‎encapsulated in the produced phospholipid system to form drug-loaded liposomes. ‎Curcumin’s ‎concentration was evaluated using UV-visible measurements. The maximal ‎amount of curcumin ‎that could be added to liposomes was assessed. Nuclear magnetic ‎resonance (NMR) analyses ‎were used to determine curcumin’s interactions and localization ‎within the phospholipid ‎membrane of the liposomes. X-ray scattering (SAXS) and atomic ‎force microscopy (AFM) ‎experiments were performed to characterize the membrane structure ‎and organization, as well as its ‎mechanical properties at the nanoscale. Conservation of the membrane’s properties is found with ‎the addition of curcumin in various ‎amounts before saturation, allowing the preparation of a ‎defined nanocarrier with desired ‎amounts of the drug.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Arab‐Tehrany

Elkhoury

Francius

et al. 2020

IJMS

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“…This system allows the analysis of soft and biological materials, when it is possible to perform high-resolution imaging under conditions conducive, such liquid and without applying vacuum. In these conditions it is possible to evaluate the influence of temperature, addition of surfactant, ions, polymers, adsorption/desorption kinetics and biomolecules-membrane interactions [57].…”

Section: Electron Microscopymentioning

confidence: 99%

Design of liposomal formulations for cell targeting

Nogueira

Gomes

Preto

et al. 2015

Colloids and Surfaces B: Biointerfaces

144

103

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Liposomes have gained extensive attention as carriers for a wide range of drugs due to being both nontoxic and biodegradable as they are composed of substances naturally occurring in biological membranes. Active targeting for cells has explored specific modification of the liposome surface by functionalizing it with specific targeting ligands in order to increase accumulation and intracellular uptake into target cells. None of the Food and Drug Administration-licensed liposomes or lipid nanoparticles are coated with ligands or target moieties to delivery for homing drugs to target tissues, cells or subcellular organelles. Targeted therapies (with or without controlled drug release) are an emerging and relevant research area. Despite of the numerous liposomes reviews published in the last decades, this area is in constant development. Updates urgently needed to integrate new advances in targeted liposomes research. This review highlights the evolution of liposomes from passive to active targeting and challenges in the development of targeted liposomes for specific therapies.

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“…This type of difference in measurements using different techniques has been already 403 reported. For instance, for EspB and EspD, two hemolytic proteins produced by the 404 type III Secretion System from E.coli, osmoprotection assays revealed a minimal pore-405 size of 3-5 nm whilst AFM showed pores ranging between 55-65 nm [45]. 406 It was quite conspicuous that in liposomes we could visualize pores much larger 407 in diameters than in erythrocytes.…”

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confidence: 99%

Direct visualization of membrane-spanning pores formed by a Leishmania amazonensis pore-forming cytolysin, as probed by atomic force microscopy

Castro-Gomes

Vilela

Andrade

et al. 2019

Preprint

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26We have previously shown that Leishmania amazonensis produces and secretes a 27 cytolysin that lyses membranes of mammalian cells, including macrophages, its host 28 cell. Using the patch-clamp technique, we have previously demonstrated that the 29 mechanism by which this cytolysin rupture macrophages plasma membrane is by pore 30 formation, which lead us to name it leishporin. While we have characterized leishporin 31 in several aspects, its molecular identity is still unknown. Its behavior suggests that 32 leishporin is, or depend on, a protein, but recent results also suggests that a non-protein 33 molecule is involved in cell lysis. Although the patch-clamp has undeniably revealed 34 that L. amazonensis extracts generates pores in macrophages, these structures have not 35 been spotted on cell membranes, which prompted us to several questions: 1) What is the 36 appearance of leishporin-induced pores? Is it similar to that of other described pores? 2) 37 Do these pores physically span lipid bilayers? 4) Are their directly-measured sizes 38 compatible with those previously suggested by patch-clamp? 5) Do these pores fuse 39 with one another, enlarging in size, as suggested by our previous reports? In the present 40 work, we have used two membrane models, erythrocytes and liposomes, to visualize 41 pores induced by the cytolysin on parasite extracts. Leishporin-mediated lysed 42 erythrocytes or liposomes were analyzed by atomic force microscopy (AFM), which 43 allowed us to visualize multiple membrane-spanning pores of variable diameters, 44 ranging from 25 to 230 nm. They do not resemble to protein-formed pores, but rather, to 45 pores made by small molecules such as lipids or peptides, as also visualized by AFM. 46 Our results suggest that the maximum size for individual pores formed by leishporin is 47 around 32 nm, but indicate that they are prone to coalesce, originating large membrane 48 damages that leads to cell collapse, what seems to be a unique property among pore-49 forming cytolysins. 3 50Author summary 51 One of the mechanisms whereby a cell can be destroyed is by punching holes into their 52 membranes. Through these holes, due to differences in osmolarity between the outside 53 and the inside of a cell, water flows towards the cytoplasm causing plasma membrane 54 ruptures, which damages or lyses cells. We have previously described in the protozoan 55 parasite Leishmania amazonensis one of such activities. Using an electrophysiology 56 technique, we have found that parasite extracts lyse cells by making pores on their 57 membranes. However these pores were not directly visualized so far. In this report, 58 using a high-resolution-type scanning microscopy, the atomic force microscopy, we 59 showed in red blood cells membranes and artificial lipid membranes (liposomes) the 60 physical aspect of the pores we described earlier. We observed that these pores are 61 circular-shaped structures with variable diameters, ranging from 25 to 230 nm that span 62 the whole thickness of both types of membra...

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Morphological and Physical Analysis of Natural Phospholipids-Based Biomembranes

Cited by 24 publications

References 31 publications

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Curcumin Loaded Nanoliposomes Localization by Nanoscale Characterization

Design of liposomal formulations for cell targeting

Direct visualization of membrane-spanning pores formed by a Leishmania amazonensis pore-forming cytolysin, as probed by atomic force microscopy

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