2020
DOI: 10.1371/journal.pone.0234125
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Morphological and physiological characterization of filamentous Lentzea aerocolonigenes: Comparison of biopellets by microscopy and flow cytometry

Abstract: Cell morphology of filamentous microorganisms is highly interesting during cultivations as it is often linked to productivity and can be influenced by process conditions. Hence, the characterization of cell morphology is of major importance to improve the understanding of industrial processes with filamentous microorganisms. For this purpose, reliable and robust methods are necessary. In this study, pellet morphology and physiology of the rebeccamycin producing filamentous actinomycete Lentzea aerocolonigenes … Show more

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Cited by 20 publications
(35 citation statements)
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References 53 publications
(68 reference statements)
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“…As the transport can now be calculated, the estimation of metabolic rates within pellets becomes, for the first time, feasible. Future experiments should provide information about the morphology and metabolic activity or nutrient profiles of pellets, which can be, for example, measured through flow cytometry or confocal laser scanning microscopy (Schrinner et al, 2020; Tegelaar et al, 2020; Veiter & Herwig, 2019). One possibility to determine the distribution of oxygen and hyphal material inside pellets would be the application of microelectrodes inside pellets (Hille et al, 2005; Wittier et al, 1986) followed by µCT measurements (Schmideder, Barthel, Friedrich, et al, 2019).…”
Section: Discussionmentioning
confidence: 99%
“…As the transport can now be calculated, the estimation of metabolic rates within pellets becomes, for the first time, feasible. Future experiments should provide information about the morphology and metabolic activity or nutrient profiles of pellets, which can be, for example, measured through flow cytometry or confocal laser scanning microscopy (Schrinner et al, 2020; Tegelaar et al, 2020; Veiter & Herwig, 2019). One possibility to determine the distribution of oxygen and hyphal material inside pellets would be the application of microelectrodes inside pellets (Hille et al, 2005; Wittier et al, 1986) followed by µCT measurements (Schmideder, Barthel, Friedrich, et al, 2019).…”
Section: Discussionmentioning
confidence: 99%
“…One of the most influential properties of filamentous microorganisms is their complex cellular morphology [16–19]. It is well‐known that there is a strong relationship between the cultivation process conditions, the cell morphology, and the productivity of filamentous microorganisms [15, 2022].…”
Section: Cell Morphology Of Filamentous Microorganismsmentioning
confidence: 99%
“…The production of citric acid [17,50], glucose oxidase [44], glucoamylase [29], and polygalacturonidase [51] is favoured in pellet growth by Aspergillus niger . Compared to mycelium cultures, pellet morphologies have also been used to achieve higher productivity in the production of nikkomycin by Streptomyces tendae [52], avermectin by Streptomyces avermitilis [53], rebeccamycin by Lentzea (formerly Lechevalieria ) aerocolonigenes [7,19,54,55], and the new peptide antibiotic labyrinthopeptin A1 by Actinomadura namibiensis [8]. For these reasons, a large number of studies focus exclusively on pellet‐like cultivations and the interaction between mechanically induced morphological changes and product formation.…”
Section: Cell Morphology Of Filamentous Microorganismsmentioning
confidence: 99%
“…The analogue becatecarin is more soluble in water and has already been tested in phase I and II clinical trials for the treatment of different types of cancer such as refractory breast cancer, metastatic colorectal cancer and small cell lung cancer [10][11][12]. Recent studies concerning L. aerocolonigenes cope with the addition of micro-or macroparticles for increased production of rebeccamycin [6,13,14]. The addition of glass beads with an average diameter of 969 mm at a concen-tration of 100 g L -1 led to a significant increase of the rebeccamycin titer [13].…”
Section: Introductionmentioning
confidence: 99%
“…Recent studies concerning L. aerocolonigenes cope with the addition of micro-or macroparticles for increased production of rebeccamycin [6,13,14]. The addition of glass beads with an average diameter of 969 mm at a concen-tration of 100 g L -1 led to a significant increase of the rebeccamycin titer [13]. The mechanical stress induced on the microorganism by the glass particles was made responsible for this effect [6].…”
Section: Introductionmentioning
confidence: 99%