Morphological and Physiological Responses of Maize (Zea mays L.) Seeds Grown under Increasing Concentrations of Chlorantraniliprole Insecticide

Kılıç, Semra; Duran, Ragbet Ezgi; Coşkun, Yasemin

doi:10.15244/pjoes/31339

Cited by 32 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The widely applied chemicals for surface sterilization of seeds of various plants are hydrogen peroxide (H 2 O 2 ), sodium hypochlorite (NaOCl), ethanol and mercuric chloride (HgCl 2 ). In the case of Zea Mays (maize) seeds, sterilization by NaOCl in concentration between 0.024% and 20% [10][11][12][13][14][15] up to 20 minutes have been reported. Surface sterilization of maize subjecting the seeds to 0.1% H 2 O 2 [16] and 10% H 2 O 2 [17] up to 30 minutes have also been proposed.…”

Section: Introductionmentioning

confidence: 99%

“…Agar plate is used as growth media for seed associated microorganisms and hence for the identification and discarding of seedlings showing microbial growth [ 6 , 29 ]. For analyzing the root development of maize following sterilization, previous studies mainly utilized filter paper [ 10 , 12 , 15 , 20 ] and only few applications of PDA have been reported [ 14 , 17 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds

et al. 2020

View full text Add to dashboard Cite

Surface sterilization of seeds is a key step in providing microorganisms-free seeds for numerous applications like understanding the role of seed-borne microorganisms in plant development, studying microbial cells-plant interactions by inoculating model microorganisms in a simplified system or selective cultivation of seed endobionts. However applying efficient treatment for surface sterilization of seeds without affecting the plant growth is not an easy task. In this study we aimed to provide an efficient surface sterilization treatment for maize seeds using i) hydrogen peroxide (HP), ii) sodium hypochlorite (SH) and iii) ethanol-sodium hypochlorite (EtOH-SH) under stirring (st) and vacuum-stirring (va-st) conditions. We used fluorescence microscopy and ultra-high resolution Helium Ion Microscopy (HIM) as powerful imaging approaches in combination with macroscopic techniques to visualize, quantify and evaluate the efficiency of seed sterilization, quality of root germination, seedlings and root hair development as well as the presence or absence of microorganisms on the root surface. Our results showed a strong reduction in microbial cell numbers of 4 orders of magnitude after the EtOH-SH treatments. Moreover, seeds exposed to EtOH-SH treatments displayed the lowest percentage of microbial growth (50%) and the highest percentage of germinated seeds (100%) compared to other sterilization treatments. HIM imaging proved the absence of microbial cells on the roots grown from seeds exposed to EtOH-SH treatments. Moreover, root hair development seemed not to be affected by any of the sterilization treatments. Our findings demonstrated that EtOH-SH treatments are significantly reducing the abundance of microbial cells from the surface of maize seeds and can be used with high confidence in future studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, pesticide application also causes phytotoxicity to plants resulting in their impaired growth and chlorophyll degradation (Sharma et al, 2015; Singh et al, 2016). Moreover, in plants under pesticide stress, secondary metabolites like phenolic compounds (Sharma et al, 2016a), carotenoids, anthocyanins, xanthophylls (Tan et al, 2012; Kilic et al, 2015; Sharma et al, 2016b), and organic acids (Ding et al, 2014) were reported to enhance.…”

Section: Introductionmentioning

confidence: 99%

Pre-sowing Seed Treatment with 24-Epibrassinolide Ameliorates Pesticide Stress in Brassica juncea L. through the Modulation of Stress Markers

et al. 2016

View full text Add to dashboard Cite

The present experiment was designed to assess the effects of seed soaking with 24-epibrassinolide (EBR) on the physiology of Brassica juncea L. seedlings grown under imidacloprid (IMI) toxicity. Application of EBR increased the length of seedlings, dry weight, and pigment contents, polyphenols, total phenols, and organic acids under IMI toxicity. The expression of genes coding key enzymes of pigment, phenols, polyphenols, and organic acid biosynthetic pathways was also studied including CHLASE (chlorophyllase), PSY (phytoene synthase), CHS (chalcone synthase) and PAL (phenylalanine ammonialyase), CS (citrate synthase), SUCLG1 (succinyl Co-A ligase,), SDH (succinate dehydrogenase), FH (fumarate hydratase), MS (malate synthase). Multiple linear regression (MLR) analysis revealed that IMI application regressed negatively on seedling length, dry weight and total chlorophyll content. However, EBR seed treatment regressed positively on all the parameters studied. Moreover, interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate, and expression of PSY and PAL. Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression of CHS and all genes studied related to organic acid metabolism. In conclusion, EBR enhanced the growth and contents of all studied metabolites by regulating the gene expression of B. juncea seedlings under IMI stress.

show abstract

“…Semra Kilic et al . studied the inhibitory effects of chlorantraniliprole on the biological properties of maize seeds . After 7 days of the germination, the most devastating inhibitory effect on germination parameters of corn seed was the length of the coleoptile (90%) at an insecticide concentration of 5.0 × 10 −4 g kg −1 .…”

Section: Resultsmentioning

confidence: 99%

Physiological effects of thiamethoxam on Zea mays and its electrochemical detection using a silver electrode

et al. 2020

View full text Add to dashboard Cite

BACKGROUND The aim of this work is the detection and quantification of bioaccumulated thiamethoxam (THM) in Zea mays at a silver electrode using square‐wave voltammetry. Thiamethoxam bioaccumulation and plant development were followed for 10 days from germination to seedling growth. Germination rate, accumulation rate, root length, and plant length were used as indicators. All experiments were carried out using several concentrations of THM (5.0 × 10−4, 1.0 × 10−3, 5.0 × 10−3, 3.4 × 10−2 and 5.0 × 10−2 mol L−1). RESULTS The results confirm that Zea mays was sensitive to this insecticide and that germination and growth inhibition were dose dependent. The efficiency and utility of the proposed method were discussed. The current intensity increases linearly with an increase in the THM bioaccumulated in Zea mays. After 10 days,the recovery results of the extraction of THM from zea Mayes samples spiked with different concentrations were encouraging. The detection and quantification limits were found to be 9.58 × 10−6 mol L−1 (3*SD/B) and 3.13 × 10−5 mol L−1 (10*SD/B). The precision was 2.67% for eight repetitions in a solution of 3.5 × 10−4 mol L−1 THM. Histological tests were also performed to confirm the effect of THM on the plant and showed that exposure to THM induced a net histological modification in the primary root tissue of Zea mays. CONCLUSION The use of THM can affect the quality of the plant crop yield, and its accumulation in edible plants could pose a potential risk for human and animal health if the insecticide intake were to exceed the recommended tolerable limits. © 2019 Society of Chemical Industry

show abstract

Morphological and Physiological Responses of Maize (Zea mays L.) Seeds Grown under Increasing Concentrations of Chlorantraniliprole Insecticide

Cited by 32 publications

References 26 publications

High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds

High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds

Pre-sowing Seed Treatment with 24-Epibrassinolide Ameliorates Pesticide Stress in Brassica juncea L. through the Modulation of Stress Markers

Physiological effects of thiamethoxam on Zea mays and its electrochemical detection using a silver electrode

Contact Info

Product

Resources

About