Morphological and physiological seed heterogeneity in the Mediterranean annual plant Tuberaria macrosepala (Cistaceae)

Zaidi, Chaneze A.; González‐Benito, M. Elena; Pérez-García, Francisco J.

doi:10.1111/j.1442-1984.2010.00279.x

Cited by 10 publications

(8 citation statements)

References 21 publications

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“…Seed heterogeneity may be associated with ecological strategies that have evolutionary significance. Morphological heterogeneity within a population may occur in seed size, shape or colour (Baskin and Baskin, 1998; Imbert, 2002; Matilla et al , 2005; Zaidi et al , 2010), and has been related to physiological properties, including dormancy (Duran and Retamal, 1989; Rodríguez et al , 2015), germination (Puga-Hermida et al , 2003; Mira et al , 2011b; 2017), and longevity (Kochanek et al , 2009; Niedzielski et al , 2009; Nagel and Borner, 2010). Seed characteristics may also vary within plant and even fruit (Venable, 1985; Guzzon et al , 2018).…”

Section: Introductionmentioning

confidence: 99%

Seed dormancy and longevity variability of Hirschfeldia incana L. during storage

Mira

Veiga-Barbosa²,

Pérez-García

2019

Seed Sci. Res.

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We studied the variability of germination, dormancy and viability loss of Hirschfeldia incana seeds in relation to seed size. Seeds were stored at 35°C under humid [75% relative humidity (RH)] or dry (33% RH) conditions. Seed germination and electrolyte leakage were evaluated periodically. Small seeds had lower longevity at humid or dry storage conditions (5 or 407 days, respectively) than large or intermediate seeds (7–9 or 536–727 days, respectively). Moreover, H. incana shows variability in seed dormancy related to seed size within a population, with small seeds having lower dormancy (13%) than intermediate (50%) or large seeds (72%). Dormancy was partially released after a short storage at 35°C and humid conditions. Under dry storage conditions, endogenous dormancy cycles were observed for over a year, and longer times of storage had a dormancy-breaking effect through dry after-ripening. Results suggest a dual strategy producing non-dormant seeds with low longevity that will germinate immediately after dispersal, and seeds with greater longevity that will delay germination. Membrane permeability increased linearly with ageing at both humid and dry storage (R2 = 0.60). Small seeds showed greater conductivity than intermediate or large seeds (0.7, 0.4 or 0.3 mS g–1 dry weight, respectively, at the 80% germination). The conductivity test could be used to evaluate the quality of H. incana seeds and would allow us to identify dormant (non-germinating) seed lots as viable. However, the influence of storage conditions and variability within a seed population on seed longevity should be taken into account when evaluating seed quality.

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Section: Introductionmentioning

confidence: 99%

Seed dormancy and longevity variability of Hirschfeldia incana L. during storage

Mira

Veiga-Barbosa²,

Pérez-García

2019

Seed Sci. Res.

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“…Also, there was a lower percentage of abnormal seedlings at 3% water content after days cryopreservation. Zaidi et al (2010) found that there were no significant differences in germination percentage due to germination time or liquid nitrogen treatment period for three different seed coat types of Tuberaria macrosepala seeds after cryopreservation compared to non-treated seeds. Interestingly, Castillo et al (2010) found significant morphological differences, e.g., for leaflet number, fruit traits and seed traits, between screen house mother Rubus plants and more than 12 years (12yr to 15 yr) cryopreserved Rubus plants.…”

Section: Phenotypic Traitsmentioning

confidence: 77%

“…Merritt et al (2014) reported that some orchid species were found with higher germination rates after ultra-low temperature (i.e., -70 o C to -196 o C) storage than those stored at higher temperature (i.e., -20 o C, 4 o C) over time periods from 12 months to 5 years. (Gonçalves and Romano, 2009;Zaidi et al, 2010;Pérez-García and González-Benito, 2008). Cejas et al (2012) also found no significant germination difference between cryopreserved seeds and control seeds for Phaseolus vulgaris.…”

Section: Resultsmentioning

confidence: 99%

“…Different plant materials have shown different results. Plants grown from cryopreserved wild tomato (Solanum lycopersicum), common bean (Phaseolus vulgaris) and Tuberaria macrosepala seeds did not show any differences for several phenotypes compared to control groups (Zevallos et al, 2014;Cejas et al, 2013;Zaidi et al, 2010). However, other researchers have found differences for some phenotypic characters.…”

Section: Introductionmentioning

confidence: 92%

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Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.)

Greene

Reid

et al. 2018

Cryobiology

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Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a -18 °C freezer (conventional storage) and storage in liquid nitrogen vapor phase at -135 to -180 °C (cryopreservation). To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. In laboratory evaluations of winter habit rye, seeds from cryopreserved samples had significantly higher normal germination percentage (P < 0.05) and lower abnormal germination percentage (P < 0.05) than those stored under conventional conditions. Cryopreserved spring habit rye also had higher normal germination percentage (P < 0.01) than conventionally stored samples. In addition, winter rye seedlings from cryopreserved seeds had longer roots and smaller root diameter (P < 0.05) than seedlings from conventionally stored seeds. In field evaluations conducted in Fort Collins, Colorado in 2014-15, spikes of plants grown from conventionally stored seeds of the winter accessions were slightly longer than those from cryopreserved seeds (P = 0.045). To detect DNA methylation changes, a methylation-sensitive amplified fragment length polymorphism (metAFLP) technique was applied to two accessions. After false discovery rate adjustment, no differences in methylation were detected between storage treatments on an individual locus basis. Our study indicated that cryopreservation slowed seed deterioration as evidenced by higher germination rates compared to conventional storage, had only minimal effects on other phenotypic traits, and had no significant effects on DNA methylation status.

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“…For some Mediterranean climate species, seeds germinate over a wide range of temperatures (e.g. 5–30 °C), once non-dormant ( Zaidi et al 2010 ; Martínez-García et al 2012 ; Copete et al 2014 ; Veiga-Barbosa and Perez-Garcia 2014 ; Martínez-Baniela et al 2016 ; Cross et al 2017 ). However, temperatures at which maximum germination occurs usually correspond with seasonal conditions that are most favourable for the growth and survival of seedlings ( Bellairs and Bell 1990 ; Mackenzie et al 2016 ), and seeds of Anigozanthos spp.…”

Section: Discussionmentioning

confidence: 99%

Seed dormancy regulates germination response to smoke and temperature in a rhizomatous evergreen perennial

Erickson

Merritt

2018

AoB PLANTS

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Seed dormancy status regulates the response of seeds to environmental cues that can trigger germination. Anigozanthos flavidus (Haemodoraceae) produces seeds with morphophysiological dormancy (MPD) that are known to germinate in response to smoke, but embryo growth dynamics and germination traits in response to temperatures and after-ripening have not been well characterized. Seeds of A. flavidus, after-ripened for 28 months at 15 °C/15 % relative humidity, were incubated on water agar, water agar containing 1 μM karrikinolide (KAR1) or 50 μM glyceronitrile at 5, 10, 15, 20, 25, 20/10 and 25/15 °C for 28 days. After incubation at 5, 10 and 25 °C for 28 days, seeds were transferred to 15 °C for another 28 days. Embryo growth dynamics were tested at 5, 10, 15 and 25 °C. Results demonstrated that fresh seeds of A. flavidus had MPD and the physiological dormancy (PD) component could be broken by either glyceronitrile or dry after-ripening. After-ripened seeds germinated to ≥80 % at 15–20 °C while no additional benefit of germination was observed in the presence of the KAR1 or glyceronitrile. Embryo length significantly increased at 10 °C, and only slightly increased at 5 °C, while growth did not occur at 25 °C. When un-germinated seeds were moved from 5–10 °C to 15 °C for a further 28 days, germination increased from 0 to >80 % in significantly less time indicating that cold stratification may play a key role in the germination process during winter and early spring in A. flavidus. The lower germination (<50 %) of seeds moved from 25 to 15 °C was produced by the induction of secondary dormancy. Induction of secondary dormancy in seeds exposed to warm stratification, a first report for Anigozanthos species, suggests that cycling of PD may be an important mechanism of controlling germination timing in the field.

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Morphological and physiological seed heterogeneity in the Mediterranean annual plant Tuberaria macrosepala (Cistaceae)

Cited by 10 publications

References 21 publications

Seed dormancy and longevity variability of Hirschfeldia incana L. during storage

Seed dormancy and longevity variability of Hirschfeldia incana L. during storage

Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.)

Seed dormancy regulates germination response to smoke and temperature in a rhizomatous evergreen perennial

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