Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding

Cázares-Raga, Febe Elena; Chávez‐Munguía, Bibiana; González‐Calixto, Cecilia; Ochoa-Franco, A.P.; Gawinowicz, Mary Ann; Rodrı́guez, Mario H.; Hernández-Hernández, Fidel de la Cruz

doi:10.1016/j.jprot.2014.07.037

Cited by 19 publications

(17 citation statements)

References 53 publications

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“…gambiae s.l. [ 6 ] Concerning Anopheles mosquitoes, signal transduction, energy metabolism, cytoskeleton, [ 31 ] signaling, translational regulation, and stress response are functions potentially involved in blood feeding, biting behavior, and sporozoite–vector interactions. [ 32,33 ] Also, after a blood meal, there is an increase in cytoskeleton function and signal transduction associated with biogenesis, which could reflect the increased biosynthetic pathways associated with oogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Mosquito energy metabolism and cytoskeleton functional categories have been associated with sporozoite–vector interactions during Plasmodium infection. [ 32,31,33 ] In addition, elevated energy metabolism and cytoskeleton functions are commonly associated with blood digestion. [ 51,34 ] In mosquito immune responses, stress response proteins were highly expressed, at the same level as the rate in immunity‐related genes.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic Investigation on Anopheles gambiae in Burkina Faso Related to Insecticide Pressures from Different Climatic Regions

et al. 2020

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In Sub-Saharan Africa, An. gambiae sensu lato (s.l.) Giles 190, largely contributes to malaria transmission. Therefore, the authors carry out a proteomic analysis to compare its metabolic state, depending on different pesticide pressures by selecting areas with/without cotton crops. The proteomes data are available via ProteomeXchange with identifier PXD016300. From a total of 1.182 identified proteins, 648 are retained for further statistical analysis and are attributed to biological functions, the most important of which being energy metabolism (120 proteins) followed by translation-biogenesis (74), cytoskeleton (71), stress response (62), biosynthetic process (60), signalling (44), cellular respiration (38), cell redox homeostasis (25), DNA processing (17), pheromone binding (10), protein folding (9), RNA processing (9), other proteins (26) and unknown functions (83). In the Sudano-Sahelian region, 421 (91.3%) proteins are found in samples from areas both with and without cotton crops. By contrast, in the Sahelian region, only 271 (55.0%) are common to both crop areas, and 233 proteins are up-regulated from the cotton area. The focus is placed on proteins with putative roles in insecticide resistance, according to literature. This study provides the first whole-body proteomic characterisation of An. gambiae s.l. in Burkina Faso, as a framework to strengthen vector control strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic Investigation on Anopheles gambiae in Burkina Faso Related to Insecticide Pressures from Different Climatic Regions

et al. 2020

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“…Based on these reports and the temporal expression pattern of peritrophins obtained in the current study, it can be presumed that the early peritrophin genes may be transcribed in response to the blood meal and their corresponding proteins are used immediately in the formation of the peritrophic matrix whereas the middle and late peritrophin gene products may be packaged into vesicles in preparation for a subsequent blood meal. Temporal regulation of secretory vesicular appearance process where PM precursors are accumulated was also demonstrated in the midgut of A. darlingi 25 , A. albimanus 13 and A. stephensi 26 . In contrast, in A. aegypti, the vesicles are formed de novo after blood feeding 19 .…”

mentioning

confidence: 72%

“…These proteins possess water retaining capability and hence protect the tissue from dehydration and provide lubrication for the food passage 3 . 13 and Aedes aegypti 14 . The PM of hematophagous insects, particularly mosquitoes, has been demonstrated to have an added role of limiting Plasmodium establishment in the mosquito midgut.…”

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confidence: 99%

Modulation of Midgut Peritrophins' Expression during <i>Plasmodium Infection</i> in <i>Anopheles stephensi</i> (Diptera:Culicidae)

et al. 2017

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“…A confidence interval >95% was used for protein identification (Unused ProtScore >1.3). Other groups of protein spots were analyzed by ESI–LC–MS/MS, at the Protein Core Facility of the Columbia University Medical Center, New York, NY, USA as described (Cázares-Raga et al, 2014). Briefly, selected protein spots were processed as before and were analyzed using a Micromass Hybrid Quadrupole/time of flight mass spectrometer (Micromass QTOF, Ultima, Waters, USA) equipped with an LC Packing nanoflow and operating in positive ion mode.…”

Section: Methodsmentioning

confidence: 99%

Protein phosphorylation during Plasmodium berghei gametogenesis

Alonso-Morales¹,

González-López²,

Cázares-Raga³

et al. 2015

Experimental Parasitology

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Plasmodium gametogenesis within the mosquito midgut is a complex differentiation process involving signaling mediated by phosphorylation, which modulate metabolic routes and protein synthesis required to complete this development. However, the mechanisms leading to gametogenesis activation are poorly understood. We analyzed protein phosphorylation during Plasmodium berghei gametogenesis in vitro in serum-free medium using bidimensional electrophoresis (2-DE) combined with immunoblotting (IB) and antibodies specific to phosphorylated serine, threonine and tyrosine. Approximately 75 protein exhibited phosphorylation changes, of which 23 were identified by mass spectrometry. These included components of the cytoskeleton, heat shock proteins, and proteins involved in DNA synthesis and signaling pathways among others. Novel phosphorylation events support a role for these proteins during gametogenesis. The phosphorylation sites of six of the identified proteins, HSP70, WD40 repeat protein msi1, enolase, actin-1 and two isoforms of large subunit of ribonucleoside reductase were investigated using TiO2 phosphopeptides enrichment and tandem mass spectrometry. In addition, transient exposure to hydroxyurea, an inhibitor of ribonucleoside reductase, impaired male gametocytes exflagellation in a dose-dependent manner, and provides a resource for functional studies.

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Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding

Cited by 19 publications

References 53 publications

Proteomic Investigation on Anopheles gambiae in Burkina Faso Related to Insecticide Pressures from Different Climatic Regions

Proteomic Investigation on Anopheles gambiae in Burkina Faso Related to Insecticide Pressures from Different Climatic Regions

Modulation of Midgut Peritrophins' Expression during <i>Plasmodium Infection</i> in <i>Anopheles stephensi</i> (Diptera:Culicidae)

Protein phosphorylation during Plasmodium berghei gametogenesis

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