A seed layer-assisted chemical bath deposition (SCBD) method at low temperature has been developed to growth uniform and high crystal quality cuprous oxide (Cu 2 O) nanoparticles on transparent conductive/glass substrates. These lms were grown on indium-doped tin oxide (ITO) as seed layer which deposited on glass substrates using magnetron sputtering-RF . The ITO seed annealing process by continuous beam (CW) of CO 2 laser was used prior to growing the Cu2O nanoparticles. In this study, controlled synthesis of Cu 2 O lms were investigated by controlling the growth temperature at 55, 60, 65, and 70 o C, respectively. The products of lms were characterized in detail by using eld emission scanning electron microscopy (FESEM), X-ray diffraction (XRD). Optical properties of this Cu 2 O nanoparticles were examined UV-Vis spectroscopy. The study suggests synthesis route for developing high quality Cu 2 O nanoparticles using SCBD method for optical and electronic applications.