Morphological and ultrastructural studies on protoplast production and reversion of the higher basidiomyceteAgaricus bisporus

Mendoza, Concepción García; Cabo, Amelia Pérez; González, Ma Luisa Sánchez; Novaes-Ledieu, Monique

doi:10.1007/bf02092133

Cited by 2 publications

(1 citation statement)

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“…The second pattern resembles regeneration of Schizophyllum commune, in which the primary cell develops a cell wall that later produces a germ tube (Peberdy 1979). Regen-" eration of B. bassiana GK2016 protoplasts occurred in several phases, similar to that described for Trichoderma viride (Benitez et al 1975) in which a true germ tube emerged from the original protoplast cell and not from the end of the budding chain of cells, as was the case with T. reesei (Picataggio et al 1983) and Agaricus bisporus (Mendoza et al 1991). The features of the aberrant budding chain of cells or false germ tube may be a consequence of the cell wall lacking some components to provide structural rigidity (Benitez et al 1975).…”

Section: Discussionmentioning

confidence: 90%

Beauveria bassiana protoplast regeneration and transformation using electroporation

Pfeifer

Khachatourians

1992

Appl Microbiol Biotechnol

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Regeneration of Beauveria bassiana GK2016 protoplasts demonstrated three phases: (1) enlargement, (2) formation of a chain of budding cells, and (3) development of a true germ tube from the original protoplast. Regeneration frequencies of up to 80°7o were obtained when using the stabilizer a m m o n i u m sulphate. Using electroporation, protoplasts were transformed tO methyl 1,2-benzimidazole carbamate (MBC) resistance with the Neurospora crassa MBC-resistant fl-tubulin gene. A transformation efficiency of one to three transformants per 5 ~tg vector D N A was obtained. The MBC phenotype was stable and transformants grew in the presence of 5 Ixg MBC/ml. Southern D N A -D N A by: bridization analysis demonstrated that integration of the vector into the chromosomal D N A had occurred.

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