Morphological Assessment of the Effect of Growth Hormone on Preantral Follicles from 11-Day-Old Mice in an in Vitro Culture System

Kobayashi, Junro; Mizunuma, Hideki; Kikuchi, Nobumasa; Liu, Xiaowei; Andoh, Kazumichi; Abe, Yumiko; Yokota, Hiromi; Yamada, Kiyohiko; Ibuki, Yoshito; Hagiwara, Haruo

doi:10.1006/bbrc.1999.2072

Cited by 52 publications

(32 citation statements)

References 23 publications

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“…In vitro experiments have suggested that the effect of GH on preantral follicular growth in immature mice is independent of IGF-I (Kumar et al 1997, Liu et al 1998, Kobayashi et al 2000. Our in vivo results are in agreement with these data, since IGF-treatment did not affect preantral follicular growth in GHR/GHBP-KO mice.…”

Section: Discussionsupporting

confidence: 92%

Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice

Slot

Kastelijn²,

Bachelot³

et al. 2006

Reproduction

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GH influences female fertility. The goal of the present study was to obtain more insight into the effect of loss of GH signalling, as observed in humans suffering from Laron syndrome, on ovarian function. Therefore, serial paraffin sections of ovaries of untreated and IGF-I-treated female GH receptor knock-out (GHR/GHBP-KO) mice were examined to determine the follicular reserve and the percentage of follicular atresia in each ovary. Our observations demonstrate that the amount of primordial follicles was significantly elevated in GHR/GHBP-KO mice, while the numbers of primary, preantral and antral follicles were lower compared with wild-type values. The reduced number of healthy growing follicles in GHR/GHBP-KO mice was accompanied by a significant increase in the percentage of atretic follicles. IGF-I treatment of GHR/GHBP-KO mice for 14 days resulted in a reduced number of primordial follicles, an increased number of healthy antral follicles, and a decreased percentage of atretic follicles. The results of the present study suggest that GH may play a role, either directly or indirectly, via for instance IGF-I, in the recruitment of primordial follicles into the growing pool. Furthermore, GH seems to protect antral follicles, directly or indirectly from undergoing atresia. Reproduction (2006) 131 525-532

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Section: Discussionsupporting

confidence: 92%

Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice

Slot

Kastelijn²,

Bachelot³

et al. 2006

Reproduction

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“…Using recombinant human GH, Liu et al (1998) demonstrated that earlier phases of follicular development in mice are controlled by GH, which stimulates secondary follicle formation in vitro. Furthermore, Kobayashi et al (2000) showed a stimulatory effect of GH on the proliferation of granulosa cells in murine preantral follicles. The rate of follicular activation in control medium (α-MEM+ alone) found in this study was lower than previous studies from the same research group.…”

Section: Discussionmentioning

confidence: 91%

Effect of sequential medium on in vitro culture of goat ovarian cortical tissue

Magalhães-Padilha

Andrade

Sales

et al. 2012

Animal Reproduction Science

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A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM+ alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in the first (days 0-8) and second (days 8-16) halves of the culture period, generating 10 treatments: α-MEM+/α-MEM+, FSH/FSH, FSH/GH, FSH/FSH + GH, GH/GH, GH/FSH, GH/FSH + GH, FSH + GH/FSH + GH, FSH + GH/FSH and FSH + GH/GH. Follicle morphology, viability and ultrastructure were analyzed. After day 1 of culture, FSH treatments maintained the percentage of normal follicles similar to the fresh control. At day 16 of culture, the treatment FSH/GH showed the highest (P < 0.05) percentage of normal follicles. The ultrastructure of follicles was preserved in the fresh control and FSH/GH treatment. Follicles cultured with FSH/GH had a higher (P < 0.05) viability than α-MEM+; however the viability was lower (P < 0.05) when compared to the fresh control. The FSH/GH treatment showed the highest (P < 0.05) percentage of follicular activation and secondary follicle formation and produced the largest (P < 0.05) mean follicular diameter after 16 days of culture. In conclusion, a sequential medium supplemented with FSH followed by GH during a long-term culture maintains the survival, viability and ultrastructure of goat preantral follicles, and promotes activation and secondary follicles.

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“…In addition, GH has an important role in the growth of preantral follicles. Kobayashi et al (2000) reported that GH induces the proliferation of granulosa and theca cells in preantral follicles. Under our experimental conditions, SRIH-14 inhibited FSH, LH and GH cells which probably resulted in the slower rate of folliculogenesis at the preantral stage.…”

Section: Discussionmentioning

confidence: 99%

Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats

Nestorović

Manojlović‐Stojanoski

Ristić

et al. 2008

Histochem Cell Biol

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The effects of multiple somatostatin (SRIH-14) administration on the pituitary-ovarian axis were examined in peripubertal rats. Female Wistar rats received subcutaneously, two daily doses of 20 microg SRIH-14 per 100 g body weight (b.w.) for five consecutive days (from the 33rd to the 37th day of life). Follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) cells were examined by the peroxidase-anti-peroxidase immunocytochemical method. Changes in cell volumes, volume densities and number per unit area (mm(2)) of FSH-, LH- and GH-immunoreactive cells were evaluated by stereology and morphometry. Serum FSH and LH levels were determined by RIA. Ovaries were analyzed by simple point counting of follicles. The volumes and volume densities of FSH-, LH- and GH-immunoreactive cells were significantly decreased while their numbers per mm(2) remained unchanged. SRIH-14 induced a significant decrease in serum FSH and LH levels. In the ovary, SRIH-14 induced an increase in the number of primordial follicles, followed by a reduction in the number of small healthy growing follicles and absence of preovulatory follicles. The number of atretic follicles was unchanged. We concluded that treatment with SRIH-14 during the peripubertal period markedly inhibited pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acted by inhibiting folliculogenesis without affecting atretic processes.

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Morphological Assessment of the Effect of Growth Hormone on Preantral Follicles from 11-Day-Old Mice in an in Vitro Culture System

Cited by 52 publications

References 23 publications

Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice

Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice

Effect of sequential medium on in vitro culture of goat ovarian cortical tissue

Somatostatin-14 influences pituitary–ovarian axis in peripubertal rats

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