Morphological Changes in Diseased Cementum Layers: A Scanning Electron Microscopy Study

Bilgin., E.; Gürgan, C. A.; Arpak, M. Nejat; Bostancı, Hasan; Güven, Kadri

doi:10.1007/s00223-003-0195-1

Cited by 22 publications

(16 citation statements)

References 11 publications

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“…3c,d) and hygroscopicity (Fig. 4b) (3, 6, 49), which are all significant characteristics of the affected enthesis and cementum-dentin complex. Concretion of collagen fibers at the enthesis and within cementum can be further corroborated by the increased and varied mineral deposits, crystal maturation, and altered crystal shapes and sizes, at the diseased enthesis (Figs.…”

Section: Discussionmentioning

confidence: 94%

Elastic discontinuity due to ectopic calcification in a human fibrous joint

et al. 2013

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Disease can alter the natural ramp-like elastic gradients to steeper step-like profiles at soft-hard tissue interfaces. Prolonged function can further mediate mechanochemical events that alter biomechanical response within diseased organs. In this study a human bone-tooth fibrous joint was chosen as a model system, in which the effects of bacterial-induced disease, i.e. periodontitis, on natural elastic gradients were investigated. Specifically, the effects of ectopic biomineral, i.e. calculus, on innate chemical and elastic gradients within the diseased cementum-dentin complex in comparison to a healthy complex, both of which are fundamental parameters to load-bearing tissues, will be discussed. Complementary techniques for mapping changes in physicochemical properties as a result of disease, included micro-X-ray computed tomography, microprobe micro X-ray fluorescence imaging, transmission electron and atomic force microscopy (TEM, AFM) techniques, and AFM-based nanoindentation. Results demonstrated primary effects as derivatives of ectopic mineralization within the diseased fibrous joint. Ectopic mineralization with no cementum resorption, but altered cementum physicochemical properties with increasing X-ray attenuation, exhibited stratified concretion with increasing X-ray fluorescence counts of calcium and phosphorus elements in the extracellular matrix. These were correlated to decreased hygroscopicity, indenter displacement, and apparent strain relieving characteristics. Disease progression identified as concretion through the periodontal ligament (PDL)-cementum enthesis and sometimes the originally hygroscopic cementum-dentin junction, resulted in a significantly increased indentation elastic modulus (3.16±1.19 GPa) and a shift toward a discontinuous interface compared to healthy conditions (1.54±0.83 GPa) (Student’s t-test, p<0.05). The observed primary effects could result in secondary downstream effects, such as compromised mechanobiology at the mechanically active PDL-cementum enthesis that can catalyze disease progression.

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Section: Discussionmentioning

confidence: 94%

Elastic discontinuity due to ectopic calcification in a human fibrous joint

et al. 2013

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“…[9] To eliminate the effect of these factors, areas above the clinical attachment level as identified by a notch were designated as diseased while that below the notch were designated as healthy. Thus, each tooth served as its own control for studying the diseased and healthy cementum.…”

Section: Discussionmentioning

confidence: 99%

“…The results of this study are in agreement with an earlier study which revealed a significant decrease in the thickness of cementum on the diseased root surfaces compared to the healthy surfaces. [9]…”

Section: Discussionmentioning

confidence: 99%

“…The possible mechanisms for the collagen decrease or loss are action of collagenase and other proteolytic enzymes responsible for the breakdown of collagen in surrounding connective tissue before cementum is exposed and to the action of bacteria and salivary enzymes producing the changes after cementum is exposed to the oral environment. [9] Interruption of continuous cementum deposition during inflammatory process by altering the environment from the healthy to diseased sites affects the physiologic deposition of cementum. [9] Reduction of periodontium by marginal periodontitis may result in destruction of progenitor cells which have the potential to form essential structural components of the periodontium.…”

Section: Discussionmentioning

confidence: 99%

“…[9] Interruption of continuous cementum deposition during inflammatory process by altering the environment from the healthy to diseased sites affects the physiologic deposition of cementum. [9] Reduction of periodontium by marginal periodontitis may result in destruction of progenitor cells which have the potential to form essential structural components of the periodontium. [16] These factors could have been responsible for the decreased thickness of cementum in diseased areas of the root.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of thickness of cementum of periodontally diseased teeth of non-diabetic and type 2 diabetic patients: A scanning electron microscopy study

Gupta¹,

Galgali²,

Bavle³

et al. 2013

J Indian Soc Periodontol

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Background:It is suggested that diabetes plays an important role in tooth loss. The periodontal structure and alveolar bone architecture in diabetics have revealed alterations. However, changes in cementum of diabetics with periodontal disease have not been evaluated adequately. Thus, the aim of the study is to evaluate and compare the thickness of cementum in diseased and healthy areas of roots of teeth with chronic periodontitis from non-diabetic (group I) and type 2 diabetic patients (group II).Materials and Methods:Thirty incisors indicated for extraction as a result of chronic periodontitis from group I and group II were selected. Measurements of Probing Depth (PD) and Clinical Attachment Loss (CAL) were taken prior to extractions. Area of the root surface above the CAL (notch) was designated as diseased, while the area below the CAL (notch) was designated as healthy. The extracted tooth samples were sectioned longitudinally and were evaluated by scanning electron microscopy (SEM). Descriptive statistical analysis was performed. Results of continuous measurements were presented as Mean ± SD.Results:The results showed that in both groups the thickness of cementum in diseased areas was lower in comparison to the thickness in healthy areas. The mean thickness of cementum in both healthy and diseased areas was higher in group II compared to group I, though the differences in both were not statistically significant.Conclusion:Within the limits of the present study, group II showed greater mean thickness of cementum in both healthy and diseased areas compared to group I, though the differences were not statistically significant.

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Differences between biological and chronological age‐at‐death in human skeletal remains: A change of perspective

Couoh

2017

American J Phys Anthropol

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TCA can be used to estimate CA and its differences with BA, being less than 10 years, are similar to those found in living populations. Differences between CA and BA are due to intra-population variability, which could be the consequence of individual differences in aging. More research is needed to have confidence that under- and overestimations of BA are indicators of aging variability at the level of the individual.

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Morphological Changes in Diseased Cementum Layers: A Scanning Electron Microscopy Study

Cited by 22 publications

References 11 publications

Elastic discontinuity due to ectopic calcification in a human fibrous joint

Elastic discontinuity due to ectopic calcification in a human fibrous joint

Evaluation of thickness of cementum of periodontally diseased teeth of non-diabetic and type 2 diabetic patients: A scanning electron microscopy study

Differences between biological and chronological age‐at‐death in human skeletal remains: A change of perspective

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