Morphological changes in the enteric nervous system caused by carcinoma of the human large intestine.

Godlewski, Janusz

doi:10.2478/v10042-010-0029-8

Cited by 23 publications

(24 citation statements)

References 17 publications

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“…Our study confirms the atrophy of myenteric but not submucosal ENS plexuses in the vicinity of the CRC invasion, and its results concur with our initial microscopic observations, which showed that changes in the morphology of MPs in the wall of large intestine were more evident as compared with SPs [11]. In a search for the mechanisms of this atrophy we decided to assess the possible role in this phenomenon of apoptosis or necrosis.…”

Section: Discussionsupporting

confidence: 91%

“…Our earlier studies regarding morphological changes of the ENS in human colorectal cancer (CRC) invasion, revealed that the MPs are atrophic in the vicinity of the tumor as compared with the unchanged (tumor-uninvolved) part of the colon wall [4,11]. However, to our best knowledge, there are no publications available to date which explain the mechanisms leading to this phenomenon.…”

Section: Introductionmentioning

confidence: 99%

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Myenteric plexuses atrophy in the vicinity of colorectal cancer tissue is not caused by apoptosis or necrosis

Kozłowska

Kwiatkowski

Oponowicz

et al. 2015

Folia Histochem Cytobiol.

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Introduction. The previously performed studies showed that the presence of colorectal cancer (CRC) tumor is associated with the atrophy of myenteric plexuses in the vicinity of cancer invasion; however, the possible mechanisms of this phenomenon are not known. The aim of the present study was to determine whether the atrophic changes of the enteric nervous system (ENS) within an intestine wall of the CRC patients were caused by apoptosis or necrosis and whether they were associated with changes in the number of galanin-immunoreactive (GAL-Ir) neurons. Material and methods. Samples of the large intestine wall located close to the CRC invasion and control, distally-located part of the colon, were collected from 9 CRC patients. The size of ENS plexuses and the number of neurons were compared. Triple immunofluorescent staining was used to visualize the co-expression of caspase 3 (CASP3) or caspase 8 (CASP8) with GAL and protein gene-product 9.5 (PGP 9.5, panneuronal marker) in the submucosal and myenteric ENS plexuses. The cells expressing myeloperoxidase (MPO, marker of neutrophils) and CD68 (marker of macrophages) were detected by immunohistochemistry around/in myenteric plexuses (MPs) and in the muscularis externa of the colon wall in the vicinity of tumor invasion. Results. Myenteric plexuses in the vicinity of the CRC tissue were significantly smaller and had lower number of neurons per plexus than distantly located plexuses. The number of CASP8-and CASP3-Ir neurons in the ENS plexuses was similar in the colon wall both close to and distally from tumor invasion. The number of CASP8-Ir neurons within MPs located close to CRC invasion was higher than of CASP3-Ir neurons. The percentage of neurons co-expressing CASP8 and GAL in myenteric plexuses close and distantly from tumor was three-fold lower than of those co-expressing CASP3 and GAL. The mean number of neutrophils and macrophages inside and around myenteric plexuses located close to tumor invasion was higher or similar, respectively, as compared with adjacent muscularis externa. Conclusions. The atrophy of myenteric plexuses in the vicinity of CRC invasion is not caused by apoptosis or necrosis. The differences in the proportions of neurons expressing galanin and the studied caspases suggest as yet unknown role of this neuropeptide in the mechanisms of neuron's atrophy in MPs located close to CRC tumor.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Myenteric plexuses atrophy in the vicinity of colorectal cancer tissue is not caused by apoptosis or necrosis

Kozłowska

Kwiatkowski

Oponowicz

et al. 2015

Folia Histochem Cytobiol.

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“…A recent clinical study of therapies for colorectal cancer has revealed that enteric innervation is disrupted; in particular, enteric neurons near the region of the tumour have been found to be missing [76]. Such disturbance to ENS integrity during the course of intestinal carcinoma is implicated in the impairment of normal intestinal functions [9], and this is proposed to be responsible for patients experiencing clinical symptoms such as constipation and diarrhoea [76].…”

Section: Reviewmentioning

confidence: 99%

Netrin-1 in the developing enteric nervous system and colorectal cancer

Dass

Nurgali

2012

Trends in Molecular Medicine

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“…However, little is known about the structure of myenteric plexuses in patients with colorectal cancer (CRC). Our group showed that CRC is accompanied by atrophy of galanin-expressing MPs localized close to the tumor invasion as compared to the plexuses localized distantly from CRC tumor [12,13]. Also the age-related neurodegenerative disorders like Alzheimer and Parkinson diseases are accompanied by a loss of neurons in myenteric and submucosal plexuses [14].…”

Section: +mentioning

confidence: 79%

Ultrastructural characteristics of myenteric plexus in patients with colorectal cancer

Zauszkiewicz-Pawlak¹,

Godlewski²,

Kwiatkowski³

et al. 2017

Folia Histochem Cytobiol.

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Introduction. It has been found previously that colorectal cancer (CRC) is accompanied by atrophy of myenteric plexuses (MPs) localized close to the tumor. The aim of the study was to compare ultrastructure of MPs localized in the unchanged part of the colon wall distant to CRC tumor with the ultrastructure of MPs in the vicinity of CRC tumor. Material and methods. The present study was conducted using post-operative material derived from 11 patients with CRC. Samples of colon wall were taken from the margin of cancer invasion and from a macroscopically unchanged segment of the large intestine, immediately fixed and processed according to the standard protocol for transmission electron microscopy studies. Results. In the MPs localized in the control part of colon wall the presence of numerous unmyelinated axons and cell bodies of neurons, interstitial cells of Cajal and enteroglial cells were observed. As compared to control samples, in the MPs located close to the tumor invasion, expansion of the extracellular matrix and myelin-like structures accompanying some nerve fibers were found. The appearance of mast and plasma cells was observed within MPs in the vicinity of CRC tumor. Sporadically, apoptotic cells were present inside the MPs. Conclusions. The presence of myelin-like structures and apoptotic cells within MPs located close to tumor invasion suggests that atrophy of MPs may be caused by factors released from CRC tumor.

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Morphological changes in the enteric nervous system caused by carcinoma of the human large intestine.

Cited by 23 publications

References 17 publications

Myenteric plexuses atrophy in the vicinity of colorectal cancer tissue is not caused by apoptosis or necrosis

Myenteric plexuses atrophy in the vicinity of colorectal cancer tissue is not caused by apoptosis or necrosis

Netrin-1 in the developing enteric nervous system and colorectal cancer

Ultrastructural characteristics of myenteric plexus in patients with colorectal cancer

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