Morphological Changes in the Liver at Different Invasive Doses of Ophistorchis Felineus (Rivolta, 1884) in Rodents (Oryctolagus Cuniculus)

Abstract

A morphometric study of liver changes at different invasive doses was conducted in rodents (Oryctolagus cuniculus). In a comparative aspect, the thickness of connective tissue formed around the portal tracts and the thickness of cellular infiltrates in the same area were studied at an invasive dose of 100, 50 and 10 metacercariae of Ophistorchis felineus (Rivolta, 1884). The experiment was conducted on sexually mature male rabbits at the age of 6 months, in each observation group of 10 individuals. Clinically healthy animals were infested with Ophistorchis felineus per os metacercariae once. The intermediate stage of the parasite was isolated from a dead fish (ide, Leuciscus idus (Linnaeus, 1758) and dace fish, Leuciscus leuciscus) obtained from the Tom River in Tomsk. The invasion that took place after 1 month was confirmed by a positive analysis of feces for parasite eggs by the Ragaser and KatoMiura methods. The animals were sacrificed after 5 weeks from the period of infestation. Histological preparations of the liver were made, stained with hematoxylin and eosin, according to Van Gieson. Histological preparations were studied by light microscopy with morphometry. The results obtained characterize a twofold change in the morphometric parameters of connective tissue thickness and cellular infiltration around the portal tracts of the liver lobes with an increase in the invasive dose.