Morphological changes in the liver and kidneys of rats subjected to terminal ileum exclusion during obstructive cholestasis

Abstract

. Designed the protocol, intellectual and scientific content of the study, provided guidelines for the surgical intervention, supervised all phases of the study. ABSTRACT PURPOSE:To investigate the effects of ileal exclusion on hepatic and renal morphology in extra-hepatic cholestasis. METHODS:Twenty four rats were distributed into three groups. Group 1 (control) underwent laparotomy and laparorrhaphy. The animals in groups 2 and 3 underwent hepatic duct ligature and kept in cholestasis for four weeks. After this period, the rats in groups 2 and 3 underwent internal biliary derivation. In Group 3, the last ten centimeters of the terminal ileum were by passed and excluded. Four weeks later, histological and biochemical analysis were performed in all animals of the three groups. RESULTS:In Group 1, no abnormalities regarding hepatic morphology were observed. All animals from groups 2 and 3 presented hepatic fibrosis. No difference was observed between the two groups. No morphological differences in renal histology could be identified among the three groups. There were differences in AST (p<0.05), ALT (p<0.05), direct bilirubin (p<0.05), ƔGT (p<0.05), urea (p<0.05) and creatinine (p<0.05) in Group 3 compared to control. CONCLUSION:The distal ileum exclusion had no influence upon the hepatic and renal morphological alterations, and biochemical liver and kidney tests have worsened.

Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

“…Cholestasis may be caused because of impaired bile secretion by hepatocytes and bile duct cells (intrahepatic cholestasis) or by the obstruction of bile ducts that carry bile from the liver to the intestine (extrahepatic cholestasis). Retention of hydrophobic bile salts in cholestasis causes damage to hepatocytes, followed by liver fibrosis, and liver cirrhosis [ 3 , 4 ]. Cirrhosis is the last stage of liver disease and one of the top ten causes of death worldwide with an estimated annual incidence of 14.1 per 100,000 people [ 5 ].…”