Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

Rajeshwari, H R; Nagveni, S.; Oli, Ajay Kumar; Parashar, Deepti; Kelmani, Chandrakanth R.

doi:10.1007/s11274-009-0126-z

Cited by 15 publications

(13 citation statements)

References 10 publications

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“…Study conducted by Supardy et al [ 45 ] also reported the damaged and distorted K. pneumoniae cell after treating with 0.5 mg/mL of Halimeda discoidea extract for 12, 24, and 36 hours of treatment. The same result also were obtained by Rajeshwari et al [ 57 ] where the morphology of K. pneumoniae showed unusual shapes of expanding, swelling, shrinking, and other multiple disorientations that were absent in the control sets after treating with H. discoidea extract. In addition, the same phenomenon was reported by Derakhshan et al [ 58 ] who treated the K. pneumoniae with the cumin ( Cuminum cyminum L.) herb extract.…”

Section: Discussionsupporting

confidence: 86%

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Ramli

Radu

Shaari

et al. 2017

BioMed Research International

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The aim of this study was to determine antibacterial activity of S. polyanthum L. (salam) leaves extract foodborne pathogens. All the foodborne pathogens were inhibited after treating with extract in disk diffusion test with range 6.67 ± 0.58–9.67 ± 0.58 mm of inhibition zone. The range of MIC values was between 0.63 and 1.25 mg/mL whereas MBC values were in the range 0.63 mg/mL to 2.50 mg/mL. In time-kill curve, L. monocytogenes and P. aeruginosa were found completely killed after exposing to extract in 1 h incubation at 4x MIC. Four hours had been taken to completely kill E. coli, S. aureus, V. cholerae, and V. parahaemolyticus at 4x MIC. However, the population of K. pneumoniae, P. mirabilis, and S. typhimurium only reduced to 3 log CFU/mL. The treated cell showed cell rupture and leakage of the cell cytoplasm in SEM observation. The significant reduction of natural microflora in grapes fruit was started at 0.50% of extract at 5 min and this concentration also was parallel to sensory attributes acceptability where application of extract was accepted by the panellists until 5%. In conclusion, S. polyanthum extract exhibits antimicrobial activities and thus might be developed as natural sanitizer for washing raw food materials.

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Section: Discussionsupporting

confidence: 86%

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Ramli

Radu

Shaari

et al. 2017

BioMed Research International

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“…The change in the morphology may be due to antibiotic stress; with an increase in the cell size, the relative contact surface reduces and consequently the attachable surface for organic (antibiotic) compounds. Therefore, bigger cells can tolerate the stress conditions better than normal cells of the same species [20,13].…”

Section: Discussionmentioning

confidence: 99%

“…The mean values were calculated from SEM photographs by taking 30 bacteria per population. Statistics were calculated using the ANOVA [12,13].…”

Section: Response Of Antibiotic On S Aureus Morphologymentioning

confidence: 99%

Prevalence of Methicillin Resistant and Virulence Determinants in Clinical Isolates of Staphylococcus aureus

Chavadi¹,

Narasanna²,

Chavan³

et al. 2018

TOIDJ

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is the major threat that is a result of the uncontrolled use of antibiotics causing a huge loss in health, so understanding their prevalence is necessary as a public health measure. Objective: The aim of this study was to determine the prevalence of methicillin-resistant MRSA and virulence determinant among associated S. aureus from the clinical samples obtained from various hospital and health care centers of the Gulbarga region in India. Materials and Methods: All the collected samples were subjected for the screening of S. aureus and were further characterized by conventional and molecular methods including their antibiotic profiling. Further, the response of methicillin antibiotic on cell morphology was studied using scanning electron microscopy. Results: A total 126 S. aureus was isolated from the clinical samples which showed, 100% resistant to penicillin, 55.5% to oxacillin, 75.3% to ampicillin, 70.6% to streptomycin, 66.6% to gentamicin, 8.7% to vancomycin and 6.3% to teicoplanin. The selected MRSA strains were found to possess mecA (gene coding for penicillin-binding protein 2A) and femA (factor essential for methicillin resistance) genetic determinants in their genome with virulence determinants such as Coagulase (coa) and the X region of the protein A (spa) gene. Further, the methicillin response in resistant S. aureus showed to be enlarged and malformed on cell morphology. Conclusion: The molecular typing of clinical isolates of S. aureus in this study was highly virulent and also resistant to methicillin; this will assist health professionals to control, exploration of alternative medicines and new approaches to combat Staphylococcal infections more efficiently by using targeted therapy.

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“…This induction of cell filamentation was previously reported in other studies using sensitive and resistant isolates from different bacterial species, including Serratia marcescens , K. pneumoniae , and P. aeruginosa subjected to third-generation cephalosporins, carbapenems, and monobactams. These studies showed that inactivation of different penicillin-binding proteins may be associated with inability to complete the cell division process after replication of the bacterial mass [ 4 , 5 , 16 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…K. pneumoniae isolates presenting resistance to cefotaxime when subjected to sub-MICs of this antibiotic were shown to cause damage to cell surfaces. Filamentation through a dose-dependent adaptive process was observed, caused by the stressful environment that was induced by the presence of the antibiotic, thereby contributing towards the therapeutic effects [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla_SHV, bla_TEM, bla_CTX-M, or bla_KPC When Subject to β‐Lactam Antibiotics

Veras

Lópes

Silva

et al. 2015

The Scientific World Journal

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The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.

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Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

Cited by 15 publications

References 10 publications

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Prevalence of Methicillin Resistant and Virulence Determinants in Clinical Isolates of Staphylococcus aureus

Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla_SHV, bla_TEM, bla_CTX-M, or bla_KPC When Subject to β‐Lactam Antibiotics

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Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

Cited by 15 publications

References 10 publications

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Antibacterial Activity of Ethanolic Extract ofSyzygium polyanthumL.(Salam)Leaves against Foodborne Pathogens and Application as Food Sanitizer

Prevalence of Methicillin Resistant and Virulence Determinants in Clinical Isolates of Staphylococcus aureus

Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes blaSHV, blaTEM, blaCTX-M, or blaKPC When Subject to β‐Lactam Antibiotics

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Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla_SHV, bla_TEM, bla_CTX-M, or bla_KPC When Subject to β‐Lactam Antibiotics