Morphological Characteristic and Clinical Value of Different Types of Tumor Vessels in Breast Cancer

Nikitenko, Natalia V.; Сеньчукова, М. А.; Tomchuk, O.; Zaitsev, Nikon V.; Стадников, А. А.; Митряков, С. О.; Дегтярев, А М; Киртбая, Д В

doi:10.17650/1726-9784-2016-15-3-15-22

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…In recent studies of tumor samples taken from 66 patients with T1-2 stages of invasive breast cancer of a non-specific type were stained with hematoxylin by Mayer and eosin, as well as by immunohistochemical method using antibodies to CD34 (the focus is on the evaluation of blood capillaries based on methodological facilities). 23 However, practice of tissue staining by Azur II on semithin epoxy slices does not ask using specific markers, sources, or involving additional methods for obtaining vessel images, 11 let us observe the vascular network of microvascular beds.…”

Section: Discussionmentioning

confidence: 99%

The Study of Microvesels of Tissue Embedded in Epoxy Resins at Human Breast Cancer

Kukurtchyan¹,

Karapetyan²

2019

ECB

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Tumor vasculature is irregularly sized and arranged in a disorganized manner, where they share characteristics of arterioles, capillaries, and venules simultaneously. Studies on microvessels of tissues (from mastopathy to human breast cancer) embedded in Epoxy resins using a light optical microscopy method were performed and human breast cancer microvessel images getting on semithin epoxy slices gave a new opportunity in the studying of this disease.

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Section: Discussionmentioning

confidence: 99%

The Study of Microvesels of Tissue Embedded in Epoxy Resins at Human Breast Cancer

Kukurtchyan¹,

Karapetyan²

2019

ECB

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Effect of LHS-1269 on morphology and angiogenesis of Lewis lung carcinoma

Меркулова

Голубева

Khochenkov

et al. 2022

Rossijskij bioterapevtičeskij žurnal

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Background. The new anticancer chemical compound LHS-1269 from the class of indolocarbazoles is undergoing preclinical studies at the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia. LHS-1269 has a high antitumor activity on transplanted tumors of mice, showed high cytotoxic activity on many tumor cells lines in vitro, and has shown an inhibitory effect on vasculogenic mimicry in tumors in vitro. LHS-1269 does not affect the catalytic activity of human topoisomerases I and IIα. An antiangiogenic mechanism of the drug’s antitumor action is suggested.Aim. To evaluate the effect of LHS-1269 on the morphological features and angiogenesis of transplanted Lewis lung carcinoma (LLC) in BDF1 mice.Materials and methods. In BDF1 mice (n = 20) with transplanted LLC tumor on days 1 and 3 after 5-fold intraperitoneal administration of LHS-1269 in a single dose of 60 mg (total dose – 300 mg/kg), mice (n = 20) on days 5 and 8 after a single intravenous injection of LHS-1269 at a dose of 100 mg/kg, mice (n = 20) with transplanted LLC tumor without administration of LHS-1269 (control). The assessment of the antitumor effect was carried out according to the criterion of inhibition of tumor growth (%) and the study of the morphological features of the tumors. To assess the effect of LHS-1269 on tumor angiogenesis in LLC tumor sections, a visual calculation of the average number (density) of blood vessels and immunohistochemical detection of expression of the CD31+ endothelial marker were performed.Results. The LHS-1269 compound in animal groups with 5-fold and 1-fold use caused tumor growth inhibition – 59–70 and 67–79 %, with pronounced morphological changes and tumor cell death. There is an uneven distribution of blood vessels in tumors and surrounding tissues in all groups. LHS-1269 caused a statistically significant decrease in the average number of blood vessels in the tumor both after 5-fold and after 1-fold administration. The statistically significant decrease in the average number of blood vessels in the surrounding connective tissue tumor was observed only after 5-fold administration of the drug. The decrease in the average number of CD31+ endothelial cells after five times intraperitoneal administration was statistically insignificant compared to control (83.1 ± 8.7 and 59.6 ± 18.9, respectively). The increase in this indicator after a single intravenous injection of LHS-1269 was statistically significant.Conclusion. LHS-1269, when administered five times and once in mice, caused pronounced morphological changes in the form of damage and death of LLC tumor cells. The results of a study of angiogenesis in a tumor do not allow an unambiguous conclusion about the inhibitory effect of LHS-1269 on angiogenesis in LLC tumors in mice.

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Immunohistochemical characteristics of breast cancer, increasing the risk of local reccurence after organosaving treatment

Демидов¹,

Демидов

Сазонов³

et al. 2018

Tumors of female reproductive system

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Objective:an immunohistochemical analysis of the characteristics of a recurrent tumor in breast cancer.Materials and methods.The statistical processing of immunohistochemical analysis’ results was performed and the most frequently encountered molecular-genetic subtypes of breast cancer with the development of local relapses were formulated. The analysis used a standard immunohistochemical panel, which is the “gold standard” for diagnostic in Russia today, and includes the expression of receptors for sex hormones (estrogen and progesterone), expression of the human epidermal growth factor HER2/neu receptor gene, and the index of proliferative activity Ki-67. The 2nd stage of the work was the evaluation of the dynamics of changes in the immunohistochemical characteristics of a recurrent tumor in comparison with the primary one.Results and conclusion.The most common local recurrence provides by triple-negative subtype of breast cancer (42 %). The changes in the immunohistochemical characteristics of a recurrent tumor in comparison with the primary one affected only the index of proliferative activity Ki-67 in the direction of its increase by 12 %.

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Morphological Characteristic and Clinical Value of Different Types of Tumor Vessels in Breast Cancer

Cited by 3 publications

References 22 publications

The Study of Microvesels of Tissue Embedded in Epoxy Resins at Human Breast Cancer

The Study of Microvesels of Tissue Embedded in Epoxy Resins at Human Breast Cancer

Effect of LHS-1269 on morphology and angiogenesis of Lewis lung carcinoma

Immunohistochemical characteristics of breast cancer, increasing the risk of local reccurence after organosaving treatment

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