The aim of this study was to perform a morphometric analysis of Prussian carp individuals collected from the Moara Domnească pond in Ilfov County, Romania. This was accompanied by a physico-chemical characterization of the water, which was sampled from the same location. Consequently, we analyzed a total of 60 Prussian carp individuals that were caught in April (N = 32) and May 2023 (N = 28). For the Carassius gibelio in the study site, we provided, for the first time, data on the weight–length relationships (WLRs), the Fulton K condition factor and the biometric features, including the total length (TL), standard length (SL), head length (HL), snout length (SNL), body depth (BD), girth and ratios for the TL/HL, as well for the TL/BD and TL/SL. A negative growth type was estimated for both the samples of C. gibelio. Based on other studies and fishing reports, besides C. gibelio, there are another 11 species that belong to the Cypriniformes order, and there are two teleost members of the Perciformes order (Lepomis gibbosus and Perca fluviatilis)—these were also associated with this habitat. Water samples were collected from 12 sampling points (SP), which were subjected to physico-chemical characterizations that consisted of an assessment of the turbidity (T), pH, electrical conductivity (EC), chloride (Cl−), total hardness (TH), oxygen regime parameters, phosphate phosphorus (P-PO43−), nitrate nitrogen (N-NO3−), nitrite nitrogen (N-NO2−) and ammonium nitrogen (N-NH4+) levels. In addition, considering their characteristics (high toxicity, non-biodegradability, long-range atmospheric transport and bioaccumulation potential), a number of organochlorine pollutants (OCPs) were identified. The total hardness values presented a very significant correlation with conductivity (r = 0.5974 ***) and with pH (r = 0.5854 ***). The results allowed for the water samples to be placed into the quality classes established by legislation, and they were also discussed in relation to the fishes’ requirements.