Morphological Characteristics of the Lacrimal Apparatus in its Obstruction of Various Genesis

Abstract

Purpose: morphological assessment of the lacrimal ducts at various anatomical levels in patients with primary (PANDO) and secondary (SALDO) obstruction after radioactive iodine therapy.Methods. The material was obtained during endoscopic dacryocystorinostomy with revision of Hasner's valve in patients with PANDO (n=7) in the distal segments of the nasolacrimal duct and in patients with SALDO (n=7) after radioactive iodine therapy. During the surgery, a biopsy of Hasner's valve, as well as a biopsy of the lacrimal sac wall were performed. The resulting material was stained with hemotoxylin and eosin, alcyan blue and by Masson method. Morphological and morphometric analyses were performed in semi-automatic mode. The results of histochemical staining of sections were translated into points taking into account the area and optical density (chromogenicity) in relative units: 1 -weak (0 -0.3); 2moderate (0.3 -0.6); 3 -signi cant (>0.6). The nonparametric Mann-Whitney criterion was used for statistical analysis. The differences were considered signi cant at p<0.05.Results. The comparative morphological study both con rmed the available information concerning the radiation nature of the obstruction and allowed to quantify the brosis level of the stromal component and other lacrimal ducts structures.Conclusion. It was shown that the nasolacrimal duct sclerosis is signi cantly lower (p=0.029) in patients with SALDO than in patients with PANDO while brosis in the lacrimal sac is the same in patients of the compared groups.