Morphological characterization and pathogenicity of Colletotrichum aenigma and C. siamense causing anthracnose on Euonymus japonicus in Beijing, China

Tan, Xiaoqian; Zhao, Jianglin; Qiao, G. H.; Liu, J. J.; Hsiang, Tom; Yu, Zhihe; Qin, Wentao

doi:10.1111/ppa.13681

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Notes: Based on the results of phylogenetic analyses, two isolates (N2-1, N2-2) were in the same clade as C. aenigma (Figure 3). The morphological characteristics of the two isolates were similar to C. aenigma [54]. N2-1 and N2-2 were identified as C. aenigma with the results of the morphological and phylogenetic analyses.…”

Section: Colletotrichum Aenigma B Weir and Pr Johnstmentioning

confidence: 67%

Identifying the Biological Characteristics of Anthracnose Pathogens of Blueberries (Vaccinium corymbosum L.) in China

Feng,

Wang,

et al. 2024

Forests

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Vaccinium corymbosum L., commonly known as blueberry, is a valuable small fruit tree in terms of its economic significance and is primarily found in China within the regions of Heilongjiang and Jilin provinces. Additionally, it thrives in the areas spanning the middle and lower reaches of the Yangtze River. Beyond their economic value, blueberries play a crucial role within forest ecosystems, serving as a significant energy source and source of nutrients. Their presence contributes significantly to fostering stability, enhancing biodiversity, and acting as an indicator of environmental quality within forest ecosystems. Since August 2022, an unknown leaf disease has been found on a large scale in blueberry fields in Nanjing, Jiangsu Province, China. The disease causes leaf curling, wilting, and even early defoliation, severely reducing the yield and production value of blueberries. The pathogenicity test confirmed the virulence of the isolates (NG5-1, NG5-2, NG5-3, NG5-4, N2-1, and N2-2) against V. corymbosum. The two pathogens were identified as Colletotrichum fructicola and C. aenigma by observing the morphological characteristics of the isolates and combined with multilocus phylogenetic analyses (ITS, CAL, ACT, TUB2, ApMat, and GAPDH). Blueberry anthracnose, caused by C. aenigma, is the first report of this disease in China. The biological characteristics of C. aenigma were investigated under different conditions, including temperature, pH, light conditions, culture medium, and carbon and nitrogen sources. The optimal temperature for growth was determined to be within the range of 25–30 °C; C. aenigma exhibits optimal growth at a pH of 7–8. Mycelial growth is favored under conditions of partial light, whereas complete darkness promotes spore production. It was found that PDA medium was the most favorable medium for C. aenigma mycelial growth, and MM medium was the best medium for spore production; the most suitable carbon sources for colony growth and spore production were sorbitol and glucose, respectively, and the most suitable nitrogen source was peptone. This study furnishes a theoretical foundation for a more scientifically informed approach to the prevention and control of anthracnose on V. corymbosum.

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Section: Colletotrichum Aenigma B Weir and Pr Johnstmentioning

confidence: 67%

Identifying the Biological Characteristics of Anthracnose Pathogens of Blueberries (Vaccinium corymbosum L.) in China

Feng,

Wang,

et al. 2024

Forests

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“…as a group are among the top ten fungal pathogens causing wilting and even death of plant parts, as well as post-harvest rotting of fruits worldwide ( Dean et al., 2012 ). For the woody ornamental plant E. japonicus , currently, only a few Colletotrichum species causing anthracnose, such as C. siamense , C. theobromicola , and C. aenigma , have been reported ( Qin et al., 2022 ; Tan et al., 2023 ). In this study, pathogenicity tests of eight species C. aenigma , C. siamense , C. theobromicola , C. gloeosporioides , C. grossum , C. fructicola , C. hebeiense and C. karstii revealed that all eight species significantly infected the leaves of E. japonicus .…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, ten Colletotrichum species have been reported to be associated with anthracnose on E. japonicus, including C. aenigma (Tan et al, 2023), C. boninense (Lee et al, 2005), C. euonymi, C. euonymicola (Lin et al, 2023), C. gigasporum (Alizadeh et al, 2015), C. gloeosporioides (Huang et al, 2016), C. karstii (Alizadeh et al, 2015), C. siamense (Wu, 2019), C. theobromicola (Qin et al, 2022) and C. trichellum (Wu, 1992) in various geographical locations. Anthracnose on E. japonicus may be caused by a variety of Colletotrichum species, but the specific dominant pathogen and its virulence are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Colletotrichum species associated with anthracnose on Euonymus japonicus and their sensitivity to fungicides

Liu,

Tan,

Zhao

et al. 2024

Front. Plant Sci.

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As an evergreen shrub, Euonymus japonicus plays a crucial role in urban landscape construction, and its growth is affected by severe foliar anthracnose caused by Colletotrichum spp. However, the biodiversity of Colletotrichum species associated with anthracnose on E. japonicus remains undetermined. This study involved a two-year collection of E. japonicus leaf samples with typical anthracnose symptoms from 9 districts in Beijing, China. A total of 194 Colletotrichum isolates were obtained, and eight Colletotrichum species were subsequently identified using morphological characteristics and molecular identification with the ACT, GADPH, CHS, TUB2, and CAL genes, as well as the rDNA-ITS region. These species included Colletotrichum aenigma, C. fructicola, C. gloeosporioides, C. grossum, C. hebeiense, C. karstii, C. siamense, and C. theobromicola with C. siamense being the most prevalent (57%), followed by C. aenigma and C. theobromicola. Furthermore, C. fructicola, C. grossum and C. hebeiense are reported for the first time as causal agents of anthracnose on E. japonicus worldwide, and C. karstii is newly reported to be associated with E. japonicus anthracnose in China. Pathogenicity tests revealed that all tested isolates exhibited pathogenicity in the presence of wounds, emphasizing the need to avoid artificial or mechanical wounds to prevent infection in E. japonicus management. The EC50 values of five fungicides, namely difenoconazole, flusilazole, tebuconazole, hexaconazole, and prochloraz, were found to be less than 10 mg/L, indicating their strong potential for application. Notably, the EC50 of prochloraz was less than 0.05 mg/L for C. theobromicola. These findings offer valuable insights for the management of anthracnose on E. japonicus.

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Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan

Poti,

Kisaki,

Arita

et al. 2024

J Gen Plant Pathol

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Morphological characterization and pathogenicity of Colletotrichum aenigma and C. siamense causing anthracnose on Euonymus japonicus in Beijing, China

Cited by 6 publications

References 32 publications

Identifying the Biological Characteristics of Anthracnose Pathogens of Blueberries (Vaccinium corymbosum L.) in China

Identifying the Biological Characteristics of Anthracnose Pathogens of Blueberries (Vaccinium corymbosum L.) in China

Diversity of Colletotrichum species associated with anthracnose on Euonymus japonicus and their sensitivity to fungicides

Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan

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