Morphological characterization and secondary metabolites profile of black pepper (Piper nigrum L.) genotypes from Sikkim

Gurung, Kabita; Manivannan, S.

doi:10.25081/josac.2020.v29.i2.6347

Cited by 3 publications

(2 citation statements)

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“…The observed variations in piperine contents in the red ripened fruits in this study and Takahashi's study could be attributed to differences in cultivars, extraction/detection methods, and regional disparities. To support our hypothesis, the piperine content exhibited variations ranging from 2.75% to 0.022%, as determined by HPLC analysis across 24 distinct black pepper genotypes gathered from various locations across India [35]. Additionally, Schnabel et al [27] conducted a time course of piperine accumulation in P. nigrum fruits and found no significant increase during the later stages of fruit development.…”

Section: Discussionmentioning

confidence: 51%

Comparative Transcriptome Analysis Reveals Genes Associated with Alkaloid Diversity in Javanese Long Pepper (Piper retrofractum) Fruits

Meechuen,

Pimsawang,

Chaisan

et al. 2023

IJPB

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Alkaloids are a class of secondary metabolites that play multifaceted roles in plant physiology, including defense mechanisms and interactions with other organisms. The alkaloids from Piper retrofractum (Javanese long pepper) fruits offer potential alternatives to synthetic pesticides due to their natural origin and insecticide properties. However, information on particular alkaloid biosynthesis pathways is required to enhance individual alkaloid production via metabolic engineering. Here, we perform HPLC profiling to demonstrate that fruit ripening influences the alkaloid diversity in P. retrofractum. De novo transcriptomic profiling of young, green mature, and red ripened fruits revealed that the piperine biosynthesis pathway genes were highly upregulated in the mature fruits. However, an enhanced accumulation of methyl piperate and guineensine in the ripened fruit was observed, entailing ripening-related differential gene expression to synchronize the alkaloid biosyntheses. Gene expression clustering and functional enrichment analysis identified a large group of genes involved in diverse biosynthetic processes explicitly enriched in the ripened fruits. A cohort of genes encoding for “Alkaloid Biosynthesis”, remarkably upregulated in the ripening fruits, indicates they may function directly in alkaloid diversity during a later stage of fruit development. This study provides the basis for metabolic engineering to enhance alkaloid diversity and production.

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Section: Discussionmentioning

confidence: 51%

Comparative Transcriptome Analysis Reveals Genes Associated with Alkaloid Diversity in Javanese Long Pepper (Piper retrofractum) Fruits

Meechuen,

Pimsawang,

Chaisan

et al. 2023

IJPB

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“…[ 17 ] P. nigrum is a rich source of secondary metabolites such as piperine (2.75%–0.022%), myrcene (2.094%–0.022%), alpha‐phellandrene (1.373%–0.008%), and linalool (0.834%–0.012%). [ 18 ] As per the available literature, P. nigrum leaf extract exhibited significant antimicrobial and antifungal ability against E. coli , Salmonella typhi , Pseudomonas aeruginosa , Candida albicans , and Staphylococcus aureus . [ 19 ] P. nigurm was also reported to exhibit antioxidant activity [ 20 ] (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Green synthesis of copper oxide nanoparticles: Characterization and applications for environmental and biomedical fields

Naaz,

Shet,

Mubarak

2023

Can J Chem Eng

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In recent years, there has been an increasing interest in the development of plant‐based nanoparticles due to their numerous benefits over conventional physio‐chemical methods, including sustainability and environmental safety. Green synthesis, a process that produces safe and sustainable goods without the use of harsh chemicals or other harmful processes, is gaining popularity. The current study focuses on the green synthesis of copper oxide nanoparticles using Piper nigrum leaf extracts, their characterization, and applications. The synthesis of nanoparticles was confirmed by changes in colour, further endorsed by UV–visible spectroscopy. Copper oxide (CuO) nanoparticles were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). CuO nanoparticle sizes ranged between 58.23 and 69.89 nm and were spherical in shape. FTIR results indicated a functional group capped on the nanoparticle surface. The antibacterial activity of the copper oxide nanoparticles was tested, and they exhibited the significant decrease in bacterial concentration and the largest zone of inhibition, making them an efficient disinfectant. Antimicrobial activity against Bacillus subtilis and Escherichia coli was observed. Furthermore, the synthesized CuO nanoparticles exhibited a high affinity for safranin dyes and demonstrated maximum removal efficiency. This makes them an effective agent for removing dyes in wastewater from industries such as clothing manufacturing. Safranin dye was successfully removed with an efficiency of 78% using nanoparticles. In conclusion, the green synthesis of copper oxide nanoparticles using plant extracts presents an eco‐friendly and sustainable approach for producing nanoparticles with a wide range of potential applications.

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Assessment of genetic diversity based on morphological, biochemical and physiological traits of black pepper (Piper nigrum L.) cultivars from Kerala

Reshma,

Sree,

Nair

2024

Preprint

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Black pepper landraces and local cultivars maintained by farmers over centuries in different agroclimatic conditions in Kerala are useful for utilizing valuable allelic diversity in breeding programs. A survey conducted in the black pepper growing regions of Kerala identified 21 genotypes with superior yield and distinct characters. Observation of qualitative morphological traits revealed moderate variability among the black pepper genotypes. The dendrogram constructed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method grouped 21 genotypes into 15 clusters at 73% genetic similarity and the similarity coefficient ranged between 0.47 and 0.81. Biochemical evaluation revealed considerable variations in their piperine (3.0–5.6%), essential oil (3.0–5.6%) and oleoresin (6.3–13.2%) contents. The principal component analysis could describe the evaluated traits as nine main and independent components, which were able to justify 86.59% of the total variance. Eigenvalues indicated that a majority of yield-related traits, viz., leaf petiole length, leaf length, leaf width, fresh spike yield, fresh berry yield and dry yield and physiological traits such as leaf thickness, relative water content and epicuticular wax, contributed to the total diversity of the black pepper germplasms. Based on the present investigation, genotypes such as G16, G15, G21, G1, G5 and G4 with considerable genetic divergence and desired qualities can be employed as good candidates in breeding programs.

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Morphological characterization and secondary metabolites profile of black pepper (Piper nigrum L.) genotypes from Sikkim

Cited by 3 publications

References 0 publications

Comparative Transcriptome Analysis Reveals Genes Associated with Alkaloid Diversity in Javanese Long Pepper (Piper retrofractum) Fruits

Comparative Transcriptome Analysis Reveals Genes Associated with Alkaloid Diversity in Javanese Long Pepper (Piper retrofractum) Fruits

Green synthesis of copper oxide nanoparticles: Characterization and applications for environmental and biomedical fields

Assessment of genetic diversity based on morphological, biochemical and physiological traits of black pepper (Piper nigrum L.) cultivars from Kerala

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