Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum L. cultivars

Labra, Massimo; Miele, Mariangela; Ledda, Bernardetta; Grassi, F.; Mazzei, Mauro; Sala, F.

doi:10.1016/j.plantsci.2004.04.026

Cited by 208 publications

(103 citation statements)

References 23 publications

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“…Grayer et al (1996) suggested a combination of major essential oil components rather than a single and dominant compound as a basis of defining chemotypes. Similar argument was pointed out by Labra et al (2004) by mentioning that chemotype determination should not be based on a single essential oil component since plants generally have two or more major compounds of relatively equal amounts. The use of principal component analysis in determining chemotypes has been suggested by Boushama et al (2006) for Lavanduladentata.…”

Section: Discussionsupporting

confidence: 58%

Chemotaxonomy of Indonesian <i>Citrus maxima</i> based on Leaf Essential Oils

Susandarini

Rugayah

Nugroho

et al. 2016

OnLine Journal of Biological Sciences

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Pummelo (Citrus maxima) is one of true citrus species originated from South East Asian regions. Pummelo is known as having high morphological diversity, but lacking of comprehensive publications documenting chemical diversity. The objectives of this study were to reveal essential oil diversity and its implication on the chemotaxonomy of the species. Leaves from forty individual plants were collected from seven districts representing pummelo distribution areas in Indonesia. Isolation of essential oils was done using maceration and solvent extraction procedures. Qualitative and quantitative analysis of the essential oils was conducted using GC-MS. Thirty one compounds were identified, consisted of monoterpenes, sesquiterpenes, diterpenes, ketones, alkenes and fatty acids. Assessment on chemotaxonomical importance of essential oils was carried out using cluster analysis and principal component analysis on volatile compounds. Three chemotypes were recognized, defined as "nerol/loliolide/alloaromadendrenechemotype", "alpha-selinenechemotype" and "alphapinene/delta-carenechemotype". Three chemotypes defined in this study was the first reported chemotaxonomic analysis for Indonesian C. maxima.

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Section: Discussionsupporting

confidence: 58%

Chemotaxonomy of Indonesian <i>Citrus maxima</i> based on Leaf Essential Oils

Susandarini

Rugayah

Nugroho

et al. 2016

OnLine Journal of Biological Sciences

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“…The biggest differences appeared in the proportion of phosphorus (RAO et al 2007, DZIDA 2010, calcium and magnesium (SEIDLER-£O¯YKOWSKA et al 2009, DZIDA, 2010, and were probably induced by different amounts of mineral nutrients in the nutritional environment of the cultivated plants as well as cultivar-specific differences. The growth dynamics of basil plants, like their morphological characteristics, is highly varied (LABRA et al 2004, NURZYÑSKA-WIERDAK 2007, ABDUELRAHMAN et al 2009, SVECOVA, NEUGEBAUEROVA 2010. The leaf/stem ratio (LS) and the leaf area index (LAI) in basil are significantly affected by both a nitrogen rate (LAI) and a cultivar (LS and LAI) (SIFOLA, BARBIERI 2006), which should also be attributable to the plant's mineral balance.…”

Section: Resultsmentioning

confidence: 99%

Changes in the content of some macronutrients in basil herbage induced by different nitrogen and potassium fertilization rates

Nurzyńska-Wierdak¹,

Rożek²,

Borowski³

et al. 2012

J. Elem.

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This experiment, carried out in a greenhouse from February to May in 2008-2010, was designed to determine the effect of an increased rate of nitrogen and potassium on the content of phosphorus, calcium, magnesium, chlorine and sulphur in basil herbage as well as to trace relationships between a basil cultivar and changes in the mineral composition of the herbage as influenced by the applied rates of nutrients. The mineral composition of basil herbage was determined on the basis of an analysis of the growing substrate conducted after the harvest of the experimental plants. Two Polish cultivars of basil, called Kasia and Wala, as well as a green-leaved form popular on the domestic horticultural market, were grown from seedlings in pots (4 dm 3 ) filled with sphagnum peat of the pH between 5.5-6.0. The following amounts of nutrients were applied, expressed in g per 1 dm 3 of the growing substrate: 0.2, 0.4, 0.6, 0.9 N in the form of ammonium nitrate; 0.4, 0.8 K in the form of potassium sulphate; 0.4 P as 20% P superphosphate; 0. rate of nitrogen resulted in an increased concentration of the mineral nutrients determined in the basil herbage. The magnesium concentration in the basil herbage dry matter was not dependent on a cultivar and nitrogen rate, but increased under the influence of the higher amount of potassium applied. On the other hand, the raised amounts of potassium did not modify the concentrations of phosphorus and sulphur in the examined plant material.Key words: Ocimum basilicum L., cultivar, nitrogen and potassium fertilization. ZMIANY ZAWARTOOECI NIEKTÓRYCH MAKROELEMENTÓW W ZIELU BAZYLII POD WP£YWEM ZRÓ¯NICOWANEGŌ YWIENIA ROOELIN AZOTEM I POTASEM AbstraktCelem dooewiadczenia przeprowadzonego w okresie od lutego do maja 2008-2010 w szklarni by³o okreoelenie wp³ywu zwiêkszonej dawki azotu oraz potasu na zawartooeae fosforu, wapnia, magnezu, chloru i siarki w zielu bazylii oraz przeoeledzenie zale¿nooeci miêdzy odmian¹ bazylii a zmianami sk³adu mineralnego ziela pod wp³ywem zastosowanych dawek sk³adników pokarmowych. Analizê sk³adu mineralnego ziela bazylii oparto na analizie pod³o¿a po zbiorze rooelin dooewiadczalnych. Bazyliê dwóch polskich odmian Kasia i Wala oraz formy zielonolistnej, popularnej na krajowym rynku ogrodniczym, uprawiano z rozsady w doniczkach (4 dm Zbiór rooelin przeprowadzono na pocz¹tku kwitnienia (29 maja 2008, 25 maja 2009 i 27 maja 2010), oecinaj¹c nadziemn¹ czêoeae pêdu powy¿ej jej zdrewnia³ych fragmentów. Ziele wysuszono w temp. 70 o C, zmielono i przeznaczono do analiz chemicznych. Badane ziele bazylii okaza³o siê dobrym Ÿród³em fosforu, wapnia, chloru i siarki. Wzrastaj¹ca dawka azotu powodowa³a zwiêkszenie koncentracji badanych sk³adników mineralnych w rooelinie. Koncentracja magnezu w suchej masie ziela bazylii nie by³a uzale¿niona od odmiany oraz dawki azotu, natomiast zwiêksza³a siê pod wp³ywem wzrastaj¹cej ilooeci potasu. Zwiêkszona ilooeae potasu nie modyfikowa³a udzia³u fosforu i siarki w badanym materiale rooelinnym.S³owa kluczowe: Ocimum basilicum L., odmia...

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“…Previous studies show that there are large numbers of species and varieties falls in this genus [1][2][3][4][5] . Several authors recognize more than 60-150 species in the genus.…”

Section: Introductionmentioning

confidence: 99%

“…Regular occurrence of the interspecific hybridization within the genus, have created taxonomic challenges, leaving very little publications on basil taxonomy which follows the International Code of Botanical nomenclature [1,2] . Due to the difficulties in identifying the species, Massimo et al (2004) has concluded that identification can be optimized by combined analysis of morphological traits, essential oil composition and molecular markers [2] . Essential oils are fragrant, highly concentrated essences of plants which are considered to exemplify the soul or life-force of the plant.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Volatile Compounds from Genus Ocimum

Vani¹,

Cheng²,

Chuah³

2009

American J. of Applied Sciences

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There are distinct varieties of basil types in the genus Ocimum which makes them very special. Genus Ocimum is widespread over Asia, Africa and Central & Southern America. All basils are member of the Lamiaceae family. The colors of the leaves vary from bright green to purple-green and sometimes almost black. Fresh basil leaves have a strong and characteristic aroma, not comparable to any other spice, although there is a hint of clove traceable. Ocimum Sanctum, also addressed as Ocimum Tenuiflorum is a sacred plant in the Hindu culture and known as Tulasi in Tamil or Holy Basil in English. Meanwhile Ocimum Basilicum, known as Common or Sweet Basil has very dark green leaves. The genus Ocimum is cultivated for its remarkable essential oil which exhibits many usages such as in medicinal application, herbs, culinary, perfume for herbal toiletries, aromatherapy treatment and as flavoring agent. Due to varying essential oil profiles even within the same species, plants may often be classified as a different species as a result of different scents. In the present study, volatile constituents of Ocimum Sanctum and Ocimum Basilicum were extracted using various solvents and their chemical constituents were identified and quantified by using GC-MS in optimized conditions. The profiles of extract from both species were compared in an effort to investigate effects of seasonal variation on their chemical compositions. The predominant species in Ocimum Sanctum and Ocimum Basilicum was found to be methyl eugenol and methyl chavicol, respectively, during different months of analysis.

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Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum L. cultivars

Cited by 208 publications

References 23 publications

Chemotaxonomy of Indonesian <i>Citrus maxima</i> based on Leaf Essential Oils

Chemotaxonomy of Indonesian <i>Citrus maxima</i> based on Leaf Essential Oils

Changes in the content of some macronutrients in basil herbage induced by different nitrogen and potassium fertilization rates

Comparative Study of Volatile Compounds from Genus Ocimum

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