Morphological characterization of chicken anaemia agent (CAA)

Gelderblom, Hans R.; Kling, Simon; Lurz, Rudi; Tischer, Ilse; Bülow, Vicco von

doi:10.1007/bf01310522

Cited by 82 publications

(41 citation statements)

References 14 publications

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“…An earlier study of negatively stained preparations of the three viruses showed CAV to have a larger diameter (19.1 to 26.5 nm) than PCV-1 (16.8 to 20.7 nm) or BFDV (14 to 20.7 nm) (20). CAV also has a more distinctive surface morphology, which was interpreted visually to indicate a Tϭ3 surface lattice with 32 hexamer-pentamer clustered morphological units (8,11). Here we calculated three-dimensional maps from unstained cryopreserved specimens of CAV and PCV-2 and from a negatively stained specimen of BFDV.…”

mentioning

confidence: 94%

Comparison of the Structures of Three Circoviruses: Chicken Anemia Virus , Porcine Circovirus Type 2 , and Beak and Feather Disease Virus

Crowther

Berriman

Curran

et al. 2003

J Virol

106

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Circoviruses are small, nonenveloped icosahedral animal viruses characterized by circular singlestranded DNA genomes. Their genomes are the smallest possessed by animal viruses. Infections with circoviruses, which can lead to economically important diseases, frequently result in virus-induced damage to lymphoid tissue and immunosuppression. Within the family Circoviridae, different genera are distinguished by differences in genomic organization. Thus, Chicken anemia virus is in the genus Gyrovirus, while porcine circoviruses and Beak and feather disease virus belong to the genus Circovirus. Little is known about the structures of circoviruses. Accordingly, we investigated the structures of these three viruses with a view to determining whether they are related. Three-dimensional maps computed from electron micrographs showed that all three viruses have a T‫1؍‬ organization with capsids formed from 60 subunits. Porcine circovirus type 2 and beak and feather disease virus show similar capsid structures with flat pentameric morphological units, whereas chicken anemia virus has stikingly different protruding pentagonal trumpet-shaped units. It thus appears that the structures of viruses in the same genus are related but that those of viruses in different genera are unrelated.

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mentioning

confidence: 94%

Comparison of the Structures of Three Circoviruses: Chicken Anemia Virus , Porcine Circovirus Type 2 , and Beak and Feather Disease Virus

Crowther

Berriman

Curran

et al. 2003

J Virol

106

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“…Dual infections, however, are more serious and result in the development of aggravated clinical signs associated with other viruses (Yuasa et al, 1980;von Bulow et al, 1986;Engstrom et al, 1988;Rosenberger & Cloud, 1989a,b;De Boer et al, 1989;Jeurissen & De Boer, submitted). Gelderblom et al (1989) and Todd et al (1990a) have demonstrated that CAV particles contain circular single-stranded DNA. Recently, we have cloned a 2.3-kb circular DNA from CAV-infected chicken lymphoblastoid cell lines.…”

Section: Introductionmentioning

confidence: 99%

Detection of chicken anaemia virus by DNA hybridization and polymerase chain reaction

et al. 1992

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“…It was first isolated in Japan (Yuasa et al, 1979) and as yet remains unclassified. Recent work has shown that CAA is an icosahedral, non-enveloped virus, about 25 nm in diameter, which possesses a singlestranded circular DNA 2.3 kb in size (McNulty et al, 1989;Gelderblom et al, 1989;Todd et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Detection of chicken anaemia agent in chickens by immunofluorescence and immunoperoxidase staining

McNeilly¹,

Allan²,

Moffett³

et al. 1991

Avian Pathology

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SUMMARYAn immunofluorescent method for the detection of CAA antigens in impression smears and cryostat sections of bone marrow and thymus tisue from infected birds is described. CAA antigens were detected using both rabbit and chicken antisera to the virus. A protocol for the demonstration of CAA antigens in formalin-fixed, paraffin-embedded thymus tissue, using a biotin-streptavidin peroxidase detection system, was also developed. Treatment of tissue sections with eleven protease solutions, prior to application of primary antibody, was evaluated. Only protease types III and XIV were successful in unmasking CAA antigens while preserving good tissue morphology with no non-specific background staining.

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Morphological characterization of chicken anaemia agent (CAA)

Abstract: Chicken anaemia agent (CAA) was characterized as a virion with 25 nm in diameter, with a buoyant density in CsCl of 1.36-1.37 g/cm3, and containing a circular, single-stranded DNA genome. The virus is composed of 32 hollow morphological units representing a regular T = 3 icosahedron.

Cited by 82 publications

References 14 publications

Comparison of the Structures of Three Circoviruses: Chicken Anemia Virus , Porcine Circovirus Type 2 , and Beak and Feather Disease Virus

Comparison of the Structures of Three Circoviruses: Chicken Anemia Virus , Porcine Circovirus Type 2 , and Beak and Feather Disease Virus

Detection of chicken anaemia virus by DNA hybridization and polymerase chain reaction

Detection of chicken anaemia agent in chickens by immunofluorescence and immunoperoxidase staining

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