Morphological characterization of Phytophthora capsici isolates from Jalisco and Michoacán, Mexico

Reyes-Tena, Alfredo; Rodríguez-Alvarado, Gerardo; Pedraza‐Santos, Martha Elena; Larsen, John; Vázquez-Marrufo, Gerardo

doi:10.18781/r.mex.fit.2007-5

Cited by 1 publication

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References 32 publications

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“…Sexual compatibility was analyzed by culturing 7-day-old mycelial discs of each isolate and two strains with mating type A1 (CPV-259 and CPV-276) [ 26 , 27 ], in PDA and V8-agar. The crosses were as follows: A1-C x A2-H, A1-C x A3-O, A2-H x A3-O, A1-C x CPV-259, A2-H x CPV-259, A3-O x CPV-259, A1-C x CPV-276, A2-H x CPV-276, and A3-O x CPV-276.…”

Section: Methodsmentioning

confidence: 99%

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Morphological and Molecular Identification of Phytophthora capsici Isolates with Differential Pathogenicity in Sechium edule

Soto-Contreras,

Caamal-Chan,

Ramírez-Mosqueda

et al. 2024

Plants

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Chayote (Sechium edule) is a crop of great economic and pharmaceutical importance in Mexico. Chayote is affected by Phytophthora capsici, which causes plant wilt and fruit rot. Three isolates of P. capsici (A1-C, A2-H, and A3-O) were obtained from three producing areas in Veracruz, Mexico. Morphometric characteristics of sporangia and the colony pattern on three different media were described. They were molecularly identified by amplification of the internal transcribed spacer region (ITS) and the partial sequence of cytochrome c oxidase subunit 1 (COI), sequences that were phylogenetically analyzed. The mating type, pathogenicity in S. edule fruits, and sensitivity to metalaxyl were determined. Isolate A1-C presented the largest sporangium; all sporangia were papillated, with different morphologies and pedicel lengths. All isolates showed different colony patterns: chrysanthemum (A1-C), stellate (A2-H), and petaloid (A3-O). The topology of the phylogenetic tree was similar for the ITS region and COI gene, the sequences of the three isolates clustered with sequences of the genus Phytophthora classified in group 2b, corroborating their identity as P. capsici. The mating type of isolates A1-C and A3-O was A2 and of isolate A2-H was A1. The pathogenicity test indicated that isolate A1-C was the most virulent and with intermediate sensitivity to metalaxyl. This work suggests that P. capsici isolates from various production areas in Mexico may exhibit morphological and virulence variability.

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Section: Methodsmentioning

confidence: 99%

Section: Mating Typementioning

confidence: 99%