Morphological Characterization of Phytophthora infestans and its Growth on Different Growth Media

Raza, Waqas; Ghazanfar, Muhammad Usman; Asif, Muhammad; Haq, Ikram-ul-; Zakria, Muhammad; Al-Ani, Laith Khalil Tawfeeq

doi:10.17582/journal.sja/2022/38.4.1189.1202

Cited by 4 publications

(7 citation statements)

References 25 publications

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“…Long-term storage media for The level of mean lesion diameter of each isolate is rated on a scale P. infestans have been tested, including agarbased and whole-seed media made from materials such as corn and V8 [16]. Corn and rye-based media were shown to be the most successful at preserving P. infestans viability [24][25][26][27]. Our results also show that the P. infestans isolates can grow and sporulate on agar-based Rye agar medium, which can also be used for long-term storage.…”

Section: Resultsmentioning

confidence: 99%

“…During the late blight season of 2022, multiple locations in the Nilgiris district were visited to collect diseased potato samples. Leaves and tubers exhibiting recognizable symptoms were collected from the field [10], labelled, and transported to the laboratory for isolation and characterization. To confirm the mating type of P. infestans, a culture of the A1 mating type (BDN22-2) was acquired from the ICAR-Central Potato Research Institute (CPRI) in Shimla (HP).…”

Section: Pathogen Sourcementioning

confidence: 99%

“…The volume was then brought to 1 liter with distilled water, 18 grams of sugar, 65 milligrams of pentachloronitrobenzene, and 0.05 grams of ßsitosterol.Agar was added to the solution and boiled for 3-5 minutes, or until the agar dissolved properly. The solution was then autoclaved for 15-20 minutes at 121°C and 15 psi.Antibiotics (vancomycin at 90 mg/l, polymyxin B at 55 mg/l, ampicillin at 210 mg/l, and rifampicin at 30 mg/l) and water were mixed and added to the medium via pipette immediately before pouring the vials.For long-term storage, P. infestans isolates TNAUPI-1, TNAUPI-2, TNAUPI-3, and TNAUPI-4 were selected [10]. Glass vials containing 5 ml of Rye A agar medium were prepared.An agar plug of the isolate was obtained from the colony margin after 14 days of incubation and transferred to vials of the medium.…”

Section: Rye a Mediamentioning

confidence: 99%

“…Mycelium was harvested from an 8-10 day old P. infestans culture grown in pea broth. It was then air-dried until the mycelia were no longer wet (26). In a mortar, 5 g of mycelia were mixed with 3 ml of 10% CTAB buffer and pounded for approximately 3 minutes.…”

Section: Dna Isolationmentioning

confidence: 99%

“…Fungal growth, sporulation, and long-term preservation have all been supported by the development of several semi-synthetic and/or organic media [7]. Soybean and carrot [8], field corn [9], bean meal [10], chickpea, oat meal [11], cereal grains and V8 juice [6], and agar are just a few of the many substrates that have been used.…”

Section: Introductionmentioning

confidence: 99%

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Selective Isolation and Characterization of Phytophthora infestans from Potatoes Using Rye Agar Media in India

Rani,

Senthilraja,

Parameshwari

et al. 2023

IJECC

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Phytophthora infestans is a pathogen that causes late blight, a major disease of potatoes. The isolation of P. infestans from infected potato plants using agar media has been challenging. This study investigated the use of Rye A and Rye B agar media for the isolation of P. infestans from infected potato tubers collected from the Nilgiris district of Tamil Nadu during 2022. The media were evaluated for hyphal growth, sporangial production, oospore formation, and long-term storage of P. infestans. Phenotypic diagnosis based on cultural and morphological characteristics confirmed the identity of P. infestans. The results were confirmed by a molecular identification test using primers specific to P. infestans. Pathogenicity tests were carried out to assess the virulence of the isolates. This study provides a useful protocol for the selective isolation and characterization of P. infestans, the potato late blight pathogen.

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Section: Resultsmentioning

confidence: 99%

Section: Pathogen Sourcementioning

confidence: 99%

Section: Rye a Mediamentioning

confidence: 99%

Section: Dna Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selective Isolation and Characterization of Phytophthora infestans from Potatoes Using Rye Agar Media in India

Rani,

Senthilraja,

Parameshwari

et al. 2023

IJECC

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First Report of Phytophthora Capsici Causing Phytophthora Blight on Bell Pepper (Capsicum annuum L.) from Haryana, India

Kumar,

Srivastava,

Rana

et al. 2024

Natl. Acad. Sci. Lett.

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Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

Azimova,

Khalilov,

Kobilov

et al. 2024

Pak. J. Phytopathol.

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An isolate belonging to the genus Phytophthora was isolated from samples of infected potato plants in Uzbekistan. The morphological characteristics of the isolate were studied and the fungus Ph. infestans belonging to the type of oomycetes was determined. The colony of Phytophthora. sp isolate is colorless and the mycelium appears porous when growing. When studying the microscopic appearance, it was found that the hyphae are not septated, the width of the hypha is 2.5-4.4 μm, the width of the sporangium is 12-17 μm, and the length is 18-25 μm. It was found that P.infestans has amphigynous antheridium with oogonium and chlamydospores. To determine the exact phylogenetic status of this isolate, rRNA gene ITS region (845 bp) and cox2 (627 bp) gene were PCR amplified. The phylogeny of both marker genes showed that the isolate was Ph. Infestans and named Ph. infestans TVKT-1 due to its closeness to Ph. infestans species. The analysis of the ITS region of the rDNA gene as a barcode alone does not provide enough information for the identification of species belonging to the genus Phytophthora, for this, it is important to study the genetic analysis of oomycetes with the additional study of the cox2 gene. Compared to Phytophthora infestans species in the NCBI database, Ph. infestans TVKT-1, which we isolated, was found to have SNP mutations in the ITS region (16, 23, and 29 nucleotides) and cox2 gene (362, 364, and 365 nucleotides) nucleotide sequences.

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Morphological Characterization of Phytophthora infestans and its Growth on Different Growth Media

Cited by 4 publications

References 25 publications

Selective Isolation and Characterization of Phytophthora infestans from Potatoes Using Rye Agar Media in India

Selective Isolation and Characterization of Phytophthora infestans from Potatoes Using Rye Agar Media in India

First Report of Phytophthora Capsici Causing Phytophthora Blight on Bell Pepper (Capsicum annuum L.) from Haryana, India

Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

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