Cayenne (Capsicum frutescens L.) classified as a horticultural crop that has high economic value. The use of fertilizer can affect the maximum growth and production of cayenne plants, so it is necessary to know the right type and time. The purpose of the study was to find the best type and time of fertilizer application for the production and quality of cayenne seeds. This study used a randomized block design with two treatment factors, the first factor was the type of fertilizer, namely Gandasil B fertilizer, Bayfolan, White KNO3 and NPK Mutiara, the second factor was the time of fertilizer application, namely fertilizer application during flowering, as well as during flowering and fruit enlargement. The results showed that the interaction between the type and time of fertilizer application had a very significant effect on the total sugar content of leaves. Fertilizer treatment has a very significant effect on the total sugar content of leaves, has a significant effect on the number of fruits per plant, weight per fruit, growth uniformity, percentage of normal and abnormal sprouts, and germination. The best type of fertilizer for fruit production is Bayfolan while judging from seed quality, the best type of fertilizer for seed production is NPK Mutiara. The timing of fertilizer application had a very significant effect on the total sugar content of the leaves but had no significant effect on other variables. The best application time for seed production is fertilizer application during flowering plants and fruit enlargement.