Morphological comparison between Chrysomya rufifacies (Macquart) and Chrysomya villeneuvi Patton (Diptera: Calliphoridae) puparia, forensically important blow flies

Sukontason, Kabkaew L.; Narongchai, Paitoon; Kanchai, Chaturong; Vichairat, Karnda; Piangjai, Somsak; Boonsriwong, Worachote; Bunchu, Nophawan; Sripakdee, Duanghatai; Chaiwong, Tarinee; Kuntalue, Budsabong; Siriwattanarungsee, Sirisuda; Sukontason, Kom

doi:10.1016/j.forsciint.2006.02.012

Cited by 20 publications

(13 citation statements)

References 13 publications

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“…The anterior spiracles display spiracular ramifications in a single row, as observed for others species that belong to Chrysomya genus (Sukontason et al, 2004(Sukontason et al, , 2005(Sukontason et al, , 2006. The dorsal organ or antennae showed a dome shape, very similar to those from Musca domestica (Diptera: Muscidae) and we supposed that this structure is an olfactory receptor, as in muscids (ChuWang and Axtell, 1971).…”

Section: Discussionsupporting

confidence: 71%

“…rufifacies and C. villeneuvi had never been recorded in Brazil, but Mariluis and Schnack (1989) reported C. rufifacies in Buenos Aires, Argentina. Sukontason et al (2006) described these two species using SEM and found some differences in the puparia tubercles. According to these authors, tubercles of C. rufifacies are elongate, showing slender spines assembled only at the tip.…”

Section: Discussionmentioning

confidence: 99%

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Ultramorphological characteristics of immature stages of Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), a fly specie of forensic importance

Mendonça

Santos-Mallet

Queiroz

2010

Microscopy Res & Technique

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Forensic entomology is an area of science that serves as a tool in crime scene investigations. Usually, flies are the first insects to reach a dead body and can oviposit just a few hours after arrival. Therefore the knowledge of immature stages is essential for correct identification of the species found on corpses. Scanning electron microscopy gives detailed information about morphological characters helping to identify the immature forms and consequently serves as a tool in crime scene investigations. C. albiceps is a very important fly for forensic entomologists because its larvae are almost always present on a dead body and it is facultative predators and therefore can alter the composition of species present at the carcass. The aim of this study is to identify eggs, larvae, and puparia of C. albiceps using SEM. Eggs were elongated with the anterior region ending in a "Y" shape and the posterior end was tapered. The micropyle was a well-adorned orifice with some projections around it. The first instar larva was composed of 12 segments separated by spines. Only one spiracular opening could be seen at the posterior spiracle. Body tegument was smooth and tubercles were not seen. Antennae and maxillary palps were visible. Second and third larval instars were very similar to first instar, except for the presence of anterior spiracle. However, body tegument was composed of net-like patches and tubercles were visible. Tubercles present at the third instar larvae were robust and erect. Puparia showed a retracted cephalic region and curved tubercles.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Ultramorphological characteristics of immature stages of Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), a fly specie of forensic importance

Mendonça

Santos-Mallet

Queiroz

2010

Microscopy Res & Technique

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“…It is difficult to identify the species because of similarities in the puparia, and rearing them to adulthood may fail. Efforts have been made to identify fly puparia by means of morphological determination under light microscopy (Reiter and Wollenek 1983;Amorim and Ribeiro 2001;Whitworth 2002Whitworth , 2003, scanning electron microscopy (SEM; Kitching 1976;Liu and Greenberg 1989;Siriwattanarungsee et al 2005;Sukontason et al 2006b) or determination the cuticular hydrocarbon composition (Ye et al 2007). In the present study, we describe the technique used to pale the integument of puparia, which allows species identification and the morphometric analysis of seven fly species that are of forensic importance in Thailand.…”

Section: Introductionmentioning

confidence: 99%

Identifying fly puparia by clearing technique: application to forensic entomology

2007

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In forensic investigations, immature stages of the fly (egg, larva, or puparia) can be used as entomological evidence at death scenes, not only to estimate the postmortem interval (PMI), analyze toxic substances, and to determine the manner of death but also to indicate the movement of a corpse in homicide cases. Of these immature stages, puparia represent the longest developmental time, which makes them of useful. However, in order for forensic entomologists to use puparia effectively, it is crucial that they are able to accurately identify the species of fly found in a corpse. Typically, these puparia are similar in general appearance, being coarctate and light brown to dark brown in color, which makes identification difficult. In this study, we report on the clearing technique used to pale the integument of fly puparia, thereby allowing observation of the anterior end (second to fourth segments) and the profile of the posterior spiracle, which are important clues for identification. We used puparia of the blowfly, Chrysomya megacephala (F.), as the model species in this experiment. With placement in a 20% potassium hydroxide solution daily and mounting on a clearing medium (Permount(R), New Jersey), the profile of the posterior spiracle could be clearly examined under a light microscope beginning on the fifth day after pupation, and the number of papillae in the anterior spiracle could be counted easily starting from the ninth day. Comparison of morphological features of C. megacephala puparia with those of other blowflies (Chrysomya nigripes [Aubertin], Chrysomya rufifacies [Macquart], Chrysomya villeneuvi [Patton], Lucilia cuprina [Wiedemann], and Hemipyrellia ligurriens [Wiedemann]) and a housefly (Musca domestica L.) revealed that the anterior ends and the profiles of the posterior spiracles had markedly distinguishing characteristics. Morphometric analysis of the length and width of puparia, along with the length of the gaps between the posterior spiracles of seven fly species, displayed differences among them. This provides a key to identifying the puparia of these seven fly species.

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“…Pupa stage: The pupa is mahogany brown to dark brown in color and coarctate and barrel shaped. Morphology of puparium has been studied widely under LM [29] and SEM [30][31][32][33][34][35]. A simply key to identify the puparium of some blow fly species of Thailand was summarized by Sukontason et al [29].…”

Section: Fig 1-lateral Views Of Medically Important Blow Fly Speciesmentioning

confidence: 99%

“…A simply key to identify the puparium of some blow fly species of Thailand was summarized by Sukontason et al [29]. The main characteristics for identification include body appearance (with or without tubercle), such as the 3 rd thoracic segment to 1 st abdominal segment, distance between right and left posterior spiracles, number of bubble membranes and papillae on the anterior spiracle [29][30][31][32][33][34][35].…”

Section: Fig 1-lateral Views Of Medically Important Blow Fly Speciesmentioning

confidence: 99%

Blow Fly (Diptera: Calliphoridae) in Thailand: Distribution, Morphological Identification and Medical Importance Appraisals

Bunchu¹

2012

Int J Parasitol Res

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Abstract-The blow fly is considered to be a medically-important insect worldwide. This review is a compilation of the currently known occurrence of blow fly species in Thailand, the fly's medical importance and its morphological identification in all stages. So far, the 93 blow fly species identified belong to 9 subfamilies, including Subfamily Ameniinae, Calliphoridae, Luciliinae, Phumosiinae, Polleniinae, Bengaliinae, Auchmeromyiinae, Chrysomyinae and Rhiniinae. There are nine species including Chrysomya megacephala, Chrysomya chani, Chrysomya pinguis, Chrysomya bezziana, Achoetandrus rufifacies, Achoetandrus villeneuvi, Ceylonomyia nigripes, Hemipyrellia ligurriens and Lucilia cuprina, which have been documented already as medically important species in Thailand. According to all cited reports, C. megacephala is the most abundant species. Documents related to morphological identification of all stages of important blow fly species and their medical importance also are summarized, based upon reports from only Thailand.

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Morphological comparison between Chrysomya rufifacies (Macquart) and Chrysomya villeneuvi Patton (Diptera: Calliphoridae) puparia, forensically important blow flies

Cited by 20 publications

References 13 publications

Ultramorphological characteristics of immature stages of Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), a fly specie of forensic importance

Ultramorphological characteristics of immature stages of Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), a fly specie of forensic importance

Identifying fly puparia by clearing technique: application to forensic entomology

Blow Fly (Diptera: Calliphoridae) in Thailand: Distribution, Morphological Identification and Medical Importance Appraisals

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