Morphological, Cytological and Embryological Characterization of F1 A. cepa × A. roylei Hybrids

Chuda, Alicja; Kłosowska, Karolina; Adamus, Adela

doi:10.1515/abcsb-2015-0025

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Therefore, with the aim of obtaining images of better quality, numerous clearing protocols that render plant tissues or organs translucent without disturbing their anatomy have been described, of which the use of methyl salicylate as a clearing agent is very common for studying ovules (e.g. [6, 7, 22, 52, 61]. The clearing technique is a quick and simple method frequently used in classical embryology to analyze ovule development, embryo sac formation, embryogenesis, and seed development [5, 6, 13, 16, 17].…”

Section: Discussionmentioning

confidence: 99%

Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (Beta vulgaris L., Amaranthaceae)

et al. 2019

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Background Clearing methods allow relatively quick processing of plant material and examination of cellular structures by rendering tissues and organs translucent. They have been adapted for plant embryology, primarily to study ovule development, megasporogenesis, megagametogenesis and embryogenesis. Such clearing methods overcome several disadvantages of the conventional embedding-sectioning techniques that are arduous and time-consuming. Although numerous protocols with different clearing solutions have been described, there have been no reports to date proposing a reliable method to clear the crassinucellate ovules of the sugar beet ( Beta vulgaris L.), an economically important crop. Therefore, this study aims to find a suitable approach to improve the tissue transparency of sugar beet ovules at different developmental stages. Results We established a methyl salicylate-based protocol that significantly improved the transparency of the B. vulgaris ovule structures, which allowed us to observe the megagameto- and embryogenesis of that species. This was achieved by (1) chemical softening of the tissues; (2) vacuum pump-assisted infiltration step; (3) shaking-assisted incubation with clearing mixtures; and (4) manual removal of the chemically softened seed coat. Conclusions The effectiveness of our method is due to the strategy combining various approaches at different stages of the procedure aiming at increasing the accessibility of the internal ovule structures to the clearing solution. The results of this study may be applied in sugar beet breeding programs, and it will provide a basis for further investigation of numerous aspects of the species’ embryology. Moreover, that unique approach may be easily adapted to other species developing crassinucellate ovules. Electronic supplementary material The online version of this article (10.1186/s13007-019-0452-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (Beta vulgaris L., Amaranthaceae)

et al. 2019

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“…They display variations in traits such as disease resistance and tolerance to environmental stressors, traits that may be absent in cultivated varieties [ 3 ]. These traits can be incorporated into breeding programs to augment the adaptability and resilience of cultivated onions [ 45 , 46 , 47 ]. For instance, certain wild onion species such as A. asarense , A. roylei , A. galanthum , A. oschaninii , A. turkestanicum , A. pskemense , A. altaicum , A. farctum , A. praemixtum , A. rhabdotum , A. pskemense , and A. vavilovii demonstrate tolerance to harsh conditions such as drought, extreme temperatures, or poor soil [ 18 ].…”

Section: Onion Genetic Resourcesmentioning

confidence: 99%

Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Ochar,

Kim

2023

Plants

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Onion (Allium cepa L.) is recognized globally as a crucial vegetable crop, prized not only for its culinary applications but also for its numerous health-promoting properties. With climate change relentlessly exerting mounting challenges to agriculture, the preservation and deployment of onion germplasm has become critical to ensuring sustainable agriculture and safeguarding food security. Global onion germplasm collections function as repositories of genetic diversity, holding within them an extensive array of valuable traits or genes. These can be harnessed to develop varieties resilient to climate adversities. Therefore, detailed information concerning onion germplasm collections from various geographical regions can bolster their utility. Furthermore, an amplified understanding of the importance of fostering international and inter-institutional collaborations becomes essential. Sharing and making use of onion genetic resources can provide viable solutions to the looming agricultural challenges of the future. In this review, we have discussed the preservation and worldwide distribution of onion germplasm, along with its implications for agricultural sustainability. We have also underscored the importance of international and interinstitutional collaboration in onion germplasm collecting and conservation for agricultural sustainability.

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“…All regenerants were subjected to molecular analysis to verify their hybrid status. en, F 1 hybrids were evaluated in terms of plant morphology, pollen viability, microsporogenesis, and female gametophyte development (Chuda et al, 2015;Kiełkowska, 2012;Kiełkowska & Adamus, 2010).…”

Section: Interspecific Crosses In Alliummentioning

confidence: 99%

“…Nuclear DNA analysis of the obtained progeny revealed that 98% of the regenerants were interspecific hybrids. Genomic in situ hybridization (GISH) revealed that hybrid plants possessed eight chromosomes identified as Allium cepa and eight chromosomes identified as Allium roylei (Chuda & Adamus, 2005, 2012Chuda et al, , 2015.…”

Section: Interspecific Crosses In Alliummentioning

confidence: 99%

Tissue Culture Techniques for the Production of Interspecific Hybrids in Poland: History and Achievements

et al. 2022

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Interspecific hybridization is a significant plant evolutionary process and, concomitantly, a frequently used method to broaden the genetic variability of species and genetically improve crops. However, in distant crosses, many prezygotic and postzygotic barriers are encountered that prevent free, uncontrolled gene flow between species. Therefore, various experimental methods exploiting tissue culture, such as in vitro fertilization, embryo rescue, and protoplast fusion, have been developed to raise hybrids that cannot be obtained naturally through conventional approaches. Professor Maciej Zenkteler of the Adam Mickiewicz University in Poznań pioneered the use of tissue culture in Poland to overcome barriers to sexual crossing. In the mid-1960s, he employed in vitro pollination in which pollen grains were directly applied onto the surface of exposed ovules as the primary method to bypass prezygotic barriers and in vitro culture of isolated ovules and developing embryos (embryo rescue) to overcome post-zygotic barriers. These approaches proved effective for species possessing numerous ovules and large placentas, such as representatives of the families Caryophyllaceae, Solanaceae, and Brassicaceae. Soon thereafter, these methods were extended to other Polish institutes and universities and applied for crop improvement, such as for the production of intergeneric hybrids between Salix and Populus species, resynthesis and broadening of the genetic variability of oilseed rape ( Brassica napus L.), and the generation of new interspecific hybrids of Allium . In the late 1970s, Professor Zenkteler was the first to undertake protoplast fusion. At that time, it appeared that no incompatibility could exist at the fusion level and that somatic hybridization would supersede the possibilities offered by sexual hybridization; however, the reality turned out to be slightly harsh. Nonetheless, successful regeneration of different interspecific hybrids of potato, tomato, or gentians has been achieved by Polish research groups thus far.

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Morphological, Cytological and Embryological Characterization of F1 A. cepa × A. roylei Hybrids

Cited by 5 publications

References 23 publications

Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (Beta vulgaris L., Amaranthaceae)

Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (Beta vulgaris L., Amaranthaceae)

Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Tissue Culture Techniques for the Production of Interspecific Hybrids in Poland: History and Achievements

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