Morphological Differences between Circulating Tumor Cells from Prostate Cancer Patients and Cultured Prostate Cancer Cells

Park, Sunyoung; Ang, Richard R.; Duffy, Simon P.; Bazov, Jenny; N., Kim; Black, Peter C.; Ma, Hongshen

doi:10.1371/journal.pone.0085264

Cited by 135 publications

(125 citation statements)

References 52 publications

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“…32) In contrast to their normal cell counterparts/ hematologic cells, cancer cells have been reported to differ cytomorphologically e.g., cancer cells exhibit a higher nuclear cytoplasmic ratio, variable size, abnormal nuclear DNA content etc. [33][34] Cytomorphological deformities in CTC has been correlated to a poor clinical outcome in colorectal, prostate and metastatic breast cancers. 35) To overcome the limitation of size-based enrichment methods, additional approaches should be used to increase the probability/efficacy of CTC detection/capture.…”

Section: Biological and Pharmaceutical Bulletin Advance Publication Bmentioning

confidence: 99%

“…The images obtained from the particle size analyzer clearly indicating that all of these cancer cell lines have majorly inner diameters of greater than 10 μm. 30,33) The values of these inner diameters are summarized in Table S2 (Figure 4b). For further analyses, we used the inner diameter as a typical parameter, because a combination of inner diameter and circularity was found to be appropriate for discriminating cancer cells from normal cells.…”

Section: Biological and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

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Size-Based Differentiation of Cancer and Normal Cells by a Particle Size Analyzer Assisted by a Cell-Recognition PC Software

Shashni

Ariyasu

Takeda

et al. 2018

Biological & Pharmaceutical Bulletin

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SummaryDetection of anomalous cells such as cancer cells from normal blood cells has the potential to contribute greatly to cancer diagnosis and therapy. Conventional methods for the detection of cancer cells are usually tedious and cumbersome. Herein, we report on the use of a particle size analyzer for the convenient size-based differentiation of cancer cells from normal cells. Measurements made using a particle size analyzer revealed that size parameters for cancer cells are significantly greater (e.g., inner diameter and width) than the corresponding values for normal cells (white blood cells (WBC), lymphocytes and splenocytes), with no significant difference in shape parameters (e.g., circularity and convexity). The inner diameter of many cancer cell lines is greater than 10 μm, in contrast to normal cells. For the detection of WBC having similar size to that of cancer cells, we developed a PC software "Cancer Cell Finder" that differentiates them from cancer cells based on brightness inflection points on a cell surface. Furthermore, the aforementioned method was validated for cancer cell/clusters detection in spiked mouse blood samples (a B16 melanoma mouse xenograft model) and circulating tumor cell cluster-like particles in the cat and dog (diagnosed with cancer) blood samples. These results provide insights into the possible applicability of the use of a particle size analyzer in conjunction with PC software for the convenient detection of cancer cells in experimental and clinical samples for theranostics.

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Section: Biological and Pharmaceutical Bulletin Advance Publication Bmentioning

confidence: 99%

Section: Biological and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Size-Based Differentiation of Cancer and Normal Cells by a Particle Size Analyzer Assisted by a Cell-Recognition PC Software

Shashni

Ariyasu

Takeda

et al. 2018

Biological & Pharmaceutical Bulletin

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“…Studies of the physical properties of CTCs found that these heterogeneous cells typically have lower buoyant densities and are larger (7-30 µm) than other hematopoietic cells [43][44][45][46][47] . Despite heterogeneity, numerous CTC isolation platforms have been developed to enrich for these rare cells from blood with notable recovery and purity rates.…”

Section: Ctc Isolation Based On Physical Propertiesmentioning

confidence: 99%

Opportunities and Challenges for Pancreatic Circulating Tumor Cells

et al. 2016

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Sensitive and reproducible platforms have been developed for detection, isolation, and enrichment of circulating tumor cells (CTCs)-rare cells that enter the blood from solid tumors, including those of the breast, prostate gland, lung, pancreas, and colon. These might be used as biomarkers in diagnosis or determination of prognosis. CTCs are no longer simply detected and quantified; they are now used in ex vivo studies of anticancer agents and early detection. We review what we have recently learned about CTCs from pancreatic tumors, describing advances in their isolation and analysis and challenges to their clinical utility. We summarize technologies used to isolate CTCs from blood samples of patients with pancreatic cancer, including immunoaffinity and label-free physical attribute-based capture. We explain methods of CTC analysis and how findings from these studies might be used to detect cancer at earlier stages, monitor disease progression, and determine prognosis. We review studies that have expanded CTCs for testing of anticancer agents and how these approaches might be used to personalize treatment. Advances in the detection, isolation, and analysis of CTCs have increased our understanding of the dissemination and progression of pancreatic cancer. However, standardization of methodologies and prospective studies are needed for this emerging technology to have a significant effect on clinical care.

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“…Changes in CTC size, surface markers, and nuclear-to-cytoplasm ratio can occur due to vesicle shedding processes, 26 the epithelial-to-mesenchymal transition (EMT) 9,11,27 in which tumor cells can be reprogrammed as cancer develops, or developed cancer therapy resistance. 28 Cell lines have been shown to be too large and expresses excessive amounts of surface markers 29,30 relative to bloodstream-captured CTCs. Such acquired differences can also result in different DEP spectra, inducing shifts in the crossover frequency.…”

Section: Introductionmentioning

confidence: 99%

Automated electrorotation shows electrokinetic separation of pancreatic cancer cells is robust to acquired chemotherapy resistance, serum starvation, and EMT

Lannin

Gruber

et al. 2016

Biomicrofluidics

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We used automated electrorotation to measure the cytoplasmic permittivity, cytoplasmic conductivity, and specific membrane capacitance of pancreatic cancer cells under environmental perturbation to evaluate the effects of serum starvation, epithelial-to-mesenchymal transition, and evolution of chemotherapy resistance which may be associated with the development and dissemination of cancer. First, we compared gemcitabine-resistant BxPC3 subclones with gemcitabine-naive parental cells. Second, we serum-starved BxPC3 and PANC-1 cells and compared them to untreated counterparts. Third, we induced the epithelial-to-mesenchymal transition in PANC-1 cells and compared them to untreated PANC-1 cells. We also measured the electrorotation spectra of white blood cells isolated from a healthy donor. The properties from fit electrorotation spectra were used to compute dielectrophoresis (DEP) spectra and crossover frequencies. For all three experiments, the median crossover frequency for both treated and untreated pancreatic cancer cells remained significantly lower than the median crossover frequency for white blood cells. The robustness of the crossover frequency to these treatments indicates that DEP is a promising technique for enhancing capture of circulating cancer cells. Published by AIP Publishing. [http://dx

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Morphological Differences between Circulating Tumor Cells from Prostate Cancer Patients and Cultured Prostate Cancer Cells

Cited by 135 publications

References 52 publications

Size-Based Differentiation of Cancer and Normal Cells by a Particle Size Analyzer Assisted by a Cell-Recognition PC Software

Size-Based Differentiation of Cancer and Normal Cells by a Particle Size Analyzer Assisted by a Cell-Recognition PC Software

Opportunities and Challenges for Pancreatic Circulating Tumor Cells

Automated electrorotation shows electrokinetic separation of pancreatic cancer cells is robust to acquired chemotherapy resistance, serum starvation, and EMT

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