Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae)

Quiazon, Karl Marx A.; Yoshinaga, Tomoyoshi; Ogawa, Kazuo; Yukami, Ryuji

doi:10.1016/j.parint.2008.06.003

Cited by 64 publications

(49 citation statements)

References 27 publications

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“…2c). The ratio between esophagus and ventriculus length can be a distinguishing morphological character for differentiating A. simplex (s.s.) from A. pegreffi (Quiazon et al 2008), and our measurement data also overlapped with A. simplex (s.s.) in their studies. Morphological analyses have been used for anisakid nematode identification (Berland 1961;Grabda 1991;Iglesias et al 2008).…”

Section: Discussionsupporting

confidence: 52%

Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea

et al. 2010

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The prevalence of infection and the identification of anisakid larvae in chum salmon (Oncorhynchus keta) from the Namdae River, the east coast of Korea, were investigated. In total, 8,358 larvae were collected from 120 fish samples (male = 58 fish, female = 62 fish) in 2008. Fish samples were collected during October and November 2008. All the chum salmon samples (120/120, 100%) caught were infected with anisakid larvae with a high intensity (69.65 ± 48.58 larvae/host). They were mostly found in muscles (98.00%). Based on the morphological and the molecular analysis of PCR-RFLP and sequencing of mitochondrial DNA cox2 gene markers, these nematodes were identified as Anisakis simplex (sensu stricto) third-stage larvae. This is the first report on the molecular identification of anisakid worms from salmonid fishes in Korea. The high occurrence of anisakid worms in chum salmon may pose considerable food safety problems if they were consumed as raw or undercooked, although their commercial value is relatively lower than other salmonid species.

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Section: Discussionsupporting

confidence: 52%

Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea

et al. 2010

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“…All six parasites showed three bands of 250, 300, 370 bp which were specific to A. pegreffii. Researchers obtained the same banding pattern for A. pegreffii in different studies 5,7,14,16,22,25 . Umehara et al 5 and Fang et al 6 developed speciesspecific primers from the ITS region of rDNA and used in multiplex PCR for the rapid identification of anisakid nematodes.…”

Section: Umehara Et Almentioning

confidence: 86%

“…In PCR-RFLP analysis, primer pairs A-B and NC2-NC5 were used for the amplification of ITS region, AniF-Ani1R for 5.8 rRNA and JB3-JB4.5 for mt-CO1 genes 7,8,[14][15][16][22][23][24][25] . The most used restriction enzymes were HinfI, HhaI and TagI for the analysis of both ribosomal and mitochondrial genes 7,8,[14][15][16][22][23][24][25] 7,[14][15][16]22,25 .…”

Section: Umehara Et Almentioning

confidence: 99%

“…The most used restriction enzymes were HinfI, HhaI and TagI for the analysis of both ribosomal and mitochondrial genes 7,8,[14][15][16][22][23][24][25] 7,[14][15][16]22,25 . In this study, we used universal primers A and B for the amplification of ITS region, and HinfI for digestion of ITS products.…”

Section: Umehara Et Almentioning

confidence: 99%

“…Umehara et al 5 and Fang et al 6 developed speciesspecific primers from the ITS region of rDNA and used in multiplex PCR for the rapid identification of anisakid nematodes. Umehara et al 5 In sequencing reactions, primer pairs A-B and NC2-NC5 were used for the amplification of ITS region, AnCO1F-AnCO1R for mt-CO1 and 211F-210R for mt-CO2 genes 8,10,15,[20][21][22][23][24][25]27 . In this study, we used primer pairs JB3-JB4.5 and 211F-210R for the amplification and sequencing of mt-CO1 and mt-CO2 genes, respectively.…”

Section: Umehara Et Almentioning

confidence: 99%

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Türkiye’nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii’nin Moleküler Tanısı

Ütük¹,

Pişkin²,

Balkaya³

2009

Kafkas Univ Vet Fak Derg

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SummaryThis study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province. DNA extraction was performed on six parasites diagnosed as nematode. Species identification was done by PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes. According to the PCR and PCR-RFLP results, all parasites were identified as Anisakis pegreffii. Partial sequence of mt-CO1 gene of one randomly selected parasite was corresponding with A. simplex and mt-CO2 genes of two parasites were corresponding with A. pegreffii. With this study, A. pegreffii was molecularly detected for the first time in Turkey. Keywords: Anisakis pegreffii, Fish, PCR, PCR-RFLP, Sequencing Türkiye'nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii'nin Moleküler Tanısı ÖzetBu çalışma, Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (Trachurus trachurus) tespit edilen altı parazitin tür tanısını yapmak amacıyla planlanmıştır. Nematod olarak belirlenen altı parazitten DNA ekstraksiyonu yapılmıştır. Tür tanısı, rDNA'nın ITS bölgelerinin (ITS1, 5.8 alt ünite rRNA ve ITS2) PCR ve PCR-RFLP analizleri ve mitokondrial sitokrom c oksidaz alt ünite 1 (mt-CO1) ve mt-CO2 genlerinin kısmi olarak sekanslanması ile yapılmıştır. PCR ve PCR-RFLP sonuçlarına göre tüm parazitlerin Anisakis pegreffii olduğu belirlenmiştir. Rastgele seçilen bir parazitin mt-CO1 geninin kısmi sekans sonucu A. simplex, iki parazitin mt-CO2 geninin kısmi sekans sonuçları ise A. pegreffii ile uyuşmuştur. Bu çalışma ile A. pegreffii Türkiye'de ilk kez moleküler olarak tespit edilmiştir.

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Anisakiasis

Mattiucci

D’Amelio

2014

Helminth Infections and Their Impact on Global Public Health

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Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae)

Cited by 64 publications

References 27 publications

Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea

Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea

Türkiye’nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii’nin Moleküler Tanısı

Anisakiasis

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