Morphological Differentiation of Human Lymphocyte Subpopulations following Polyclonal Stimulation with Bacteria and Lectin

Braun, M G; Gross, Wolfgang L.; Müller-Hermelink, H. K.

doi:10.1016/s0171-2985(88)80042-1

Cited by 2 publications

(3 citation statements)

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“…One may argue that cells other than monocytes, such as lymphocytes or basophils, contribute to the parameters studied (4, 9, 32). The interaction of the bacteria with lymphocytes may induce regulatory factors which may affect the cellular susceptibility for mediator release (4,32).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years we presented evidence that mediators of inflammation such as leukotrienes, intracellular enzymes, and histamine are released from granulocytes and mast cells on interaction with E. coli expressing mannose-resistant hemagglutination (23,24,37). Leukotrienes are released from various cells on stimulation, e.g., from neutrophils, eosinophils, and monocytes via a 5-lipoxygenase (4,15,34). Activation of platelets leads via 12-lipoxygenase to 12-hydroxyeicosatetraenoic acid (12-HETE) formation (31); 12-HETE is transformed via a 5-lipoxygenase from lymphocytes, monocytes, and basophils (LMBs) to 5(S) 12(S)-DiHETE.…”

mentioning

confidence: 99%

“…Leukotriene B4 (LTB4) has been shown to be chemotactic for human neutrophils and eosinophils (4). LTB4 is metabolized by omega oxidation into 20-OHand 20-COOH-LTB4, which are biologically inactive (8).…”

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confidence: 99%

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Effects of adhesins from mannose-resistant Escherichia coli on mediator release from human lymphocytes, monocytes, and basophils and from polymorphonuclear granulocytes

et al. 1990

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We investigated the role of Escherichia coli expressing mannose-resistant hemagglutination and adhesins with regard to the induction of leukotrienes from a suspension of human lymphocytes, monocytes, and basophils (LMBs) compared with human polymorphonuclear granulocytes (PMNs). Genetically cloned E. coli strains expressing various types of mannose-resistant hemagglutination (MRH+) were phagocytosed to a higher degree by monocytes than the nonadherent E. coli strain. The various strains differed in their capacity to induce a chemiluminescence response, which showed the same pattern for LMBs and PMNs. Stimulation of LMBs with bacteria alone, unlike granulocytes, did not activate the cells for the release of leukotrienes. However, preincubation of LMBs with bacteria decreased subsequent leukotriene formation when the cells were stimulated with calcium ionophore. The inhibitory effect was dependent on the concentration of bacteria used for preincubation as well as on the preincubation temperature. The various bacterial strains differed in inhibitory potency for mediator release. Preincubation of LMBs with zymosan, opsonized zymosan, the bacterial peptide FMLP, and peptidoglycan had no inhibitory effect or even increased subsequent leukotriene formation. Opsonized bacteria were far less inhibitory than nonopsonized bacteria. In contrast to human LMBs, preincubation of human PMNs with mannose-resistant bacteria led to increased leukotriene B4 generation and reduced w-oxidation of leukotriene B4. Our data suggest that phagocytes (neutrophils, monocytes) respond in a different way for leukotriene formation after interaction with mannose-resistant E. coli.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%