Morphological diversity and DNA polymorphism of common oat (Avena sativa L.) breeding varieties cultivated in Poland

Nowosielska, D.; Nowosielski, Jarosław

doi:10.2478/v10129-010-0004-8

Cited by 5 publications

(5 citation statements)

References 3 publications

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“…The genetic diversity and the population structure of Polish oat germplasm has been analysed in the past using molecular markers [24,27,28,42,43]. Despite that, little is known about the amount and distribution of the genetic diversity and the population structure within the Polish oat landrace collection, as all above mentioned studies included only fragments of the collection.…”

Section: Discussionmentioning

confidence: 99%

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

et al. 2016

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The assessment of diversity and population structure and construction of a core collection is beneficial for the efficient use and management of germplasm. A unique collection of common oat landraces, cultivated in the temperate climate of central Europe until the end of the twentieth century, is preserved in the Polish gene bank. It consists of 91 accessions that have never been used in breeding programs. In order to optimise the use of this genetic resource, we aimed to: (1) determine genetic and agro-morphological diversity, (2) identify internal genetic variation of the tested accessions, (3) form a core collection and (4) recognise the accessions useful for breeding programs or re-release for cultivation. The collection was screened using ISSR markers (1520 loci) and eight agro-morphological traits. Uniquely, we performed molecular studies based on 24 individuals of every accession instead of bulk samples. Therefore, assessment of the degree of diversity within each population and the identification of overlapping gene pools were possible. The observed internal diversity (Nei unbiased coefficient) was in the range of 0.17–0.31. Based on combined genetic and agro-morphological data, we established the core collection composed of 21 landraces. Due to valuable compositions of important traits, some accessions were also identified as useful for breeding programs. The population structure and principal coordinate analysis revealed two major clusters. Based on the previous results, the accessions classified within the smaller one were identified as obsolete varieties instead of landraces. Our results show that the oat landraces are, in general, resistant to local races of diseases, well adapted to local conditions and, in some cases, yielding at the level of modern varieties. Therefore, in situ conservation of the landraces in the near future may be satisfactory for both farmers and researchers in terms of the genetic resources preservation.

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Section: Discussionmentioning

confidence: 99%

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

et al. 2016

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“…Results of molecular analysis of the collection of old oat cultivars were complemented by morphological description. Similar, comprehensive approach to the problem was presented by Benin et al (2008) and Nowosielska and Nowosielski (2009).…”

Section: Selection Of Techniquesmentioning

confidence: 95%

“…In the studied collection of primary cultivars seven botanical varieties such as: aristata, aurea, flava, grisea, mutica, obtustata and tartarica were identified. The collection of Polish landraces was also composed of seven botanical varieties (Nowosielska and Nowosielski 2009;Boczkowska and Tarczyk 2013), but these two collections were different. In the primary cultivars collection a very morphologically interesting accession occurred composed of individuals belonging to a varieties obtustata and tartarica (Zielony).…”

Section: Genetic Diversity Versus Morphological Variabilitymentioning

confidence: 99%

Assessing genetic diversity in 23 early Polish oat cultivars based on molecular and morphological studies

Boczkowska

Nowosielski

Nowosielska

et al. 2014

Genet Resour Crop Evol

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In Poland oat breeding began at the late nineteenth century. During the World War II almost all of Polish breeding materials were lost, and then were replaced by German cultivars. The main aim of this paper was to show the level of genetic diversity of Polish oat cultivars which were bred before 1939. Simultaneously usefulness and informativeness of molecular and morphological methods were tested and compared. This study involved 23 cultivars, which were described by 25 morphological traits and three types of molecular markers (AFLP, ISSR and RAPD). Based on Dice coefficient, genetic distance between cultivars ranged from 0.17 to 0.44. The degree of morphological differentiation within the collection varied depending on trait. Nei's genetic diversity for the combined results for the whole collection was equal to 0.202. Neither unweighted pair group method with arithmetic mean nor principal coordinate analysis showed any discrimination of cultivars according to breeding period and morphological trait. Part of morphological diversity has been preserved in the Polish early oat cultivars, do not exist anymore in contemporary cultivars, and also in landraces. The interest of breeders in early cultivars germplasm stored in genebank was and still is negligible. Breeding is confined to a few preferred by market morphotypes. So, it is very probable that the gene pools of early and contemporary cultivars could be separable.

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“…The eco-geographic conditions of collection sites were defined on the basis of geographic coordinates in the passport data (Nowosielska and Nowosielski 2008) (Appendix Table 1). Oat (Avena sativa L.) landraces were collected in five regions of Poland.…”

Section: Eco-geographymentioning

confidence: 99%

Environmentally-related genotypic, phenotypic and metabolic diversity of oat (Avena sativa L.) landraces based on 67 Polish accessions

Boczkowska

Żebrowski

Nowosielski

et al. 2017

Genet Resour Crop Evol

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Here we focused on Polish oat landraces developed in the moderate temperature zone attempting to understand their genetic and phenotypic variability in context of the pressure of environmental constrains at the locations where they had evolved. Besides molecular markers and diverse phenotypic traits the Fourier transform infrared (FTIR) spectroscopy, enabling throughput metabolic fingerprinting, was used for the description of the germplasm collection. We used sophisticated multivariable analysis to integrate the data for structure analysis and to establish mutual relationships. We found that the accessions diversity across the eco-geographical variables was manifested differently depending on selected phenotypic, genetic, and metabolic criteria.Grain metabolic fingerprint derived from FTIR spectroscopy revealed the highest degree of germplasm diversity among all of the examined traits, particularly for spectral bands assigned to lipids. The landraces, which were collected in close geographical proximity, showed clear morphological and metabolic resemblances, although they represented quite different genetic backgrounds. All three levels of analysis showed the presence of selection resulting from environmental pressures and more specifically from the temperature at the landrace origin site. This research also proved that coupling of genetic polymorphism with the FTIR fingerprinting markedly extended the description of the oat landraces diversity.

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Morphological diversity and DNA polymorphism of common oat (Avena sativa L.) breeding varieties cultivated in Poland

Cited by 5 publications

References 3 publications

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

Assessing genetic diversity in 23 early Polish oat cultivars based on molecular and morphological studies

Environmentally-related genotypic, phenotypic and metabolic diversity of oat (Avena sativa L.) landraces based on 67 Polish accessions

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