“…The traditional medicine systems use the whole plant (Bhargava et al, 2009;Tabassum et al, 2012), wherein the entire plant, including its roots is uprooted before seed setting, depriving the species of its natural regeneration potential (Aleem & Kabir, 2018). Further, the natural regeneration of chirayita is also affected due to poor seed germination and low viability (Chakraborty, Mukherjee, & Baskey, 2016;Chaudhuri, Pal, & Jha, 2007. Due to over-exploitation, unsustainable harvesting practices and habitat loss, the International Union for Conservation of Nature (IUCN) listed the chirayita as one of the critically endangered plant species (Joshi & Dhawan, 2005;Kumar & Staden, 2016).…”