Strawberry, an important berry fruit crop with high value and nutritional properties is picking up in the South Indian conditions, especially in Tamil Nadu under temperate region of Nilgiris district. Since the fruit is directly consumed by the children and all group of categories, the choice of varieties and its performance in terms of fruit yield and quality particularly sugar and acidity level will decide the marketability and preference. The field evaluation of strawberry varieties collected from different places and suitability will help the farmers to cultivate and get benefit and profitability out of the crop. A collection of nine strawberry varieties was done and assessed for the performance under greenhouse growing environment, in Nilgiris condition with randomized block design having three replications during the year 2018 to 2020 in Horticultural Research Station, TNAU, Ooty, Nilgiris.The variety Rania recorded better performance for plant height (33.81 cm), plant spread(E-W) [45.38 cm], (N-S) [43.96 cm] during 120 days after planting. Flavia recorded more number of leaves (24.80). Rania recorded a fruit length (4.05 cm), fruit width (11.29 cm), fruit weight (27.62 g). The variety Capri recorded more number of fruits per plant (68.86) which is on par with Flavia (68.80). The fruit quality parameters Total Soluble Solids (TSS) is high with 12.43o brix in Nabila and acidity (0.98 %) in Flaminia. The highest yield per plant (1190.20 g) was recorded in Flavia and with lowest yield in Capri (563.46 g). The variety Flavia recorded maximum in fruit parameters like fruit length, fruit width, fruit weight and highest yield whereas fruit quality parameters of TSS and acidity is highest in Nabila and Flaminia respectively.