Morphological evidence for cyclic AMP-induced reverse transformation in vole cells infected with avian sarcoma virus.

Abstract

(14,16,31,34). Electron microscopy shows a rearrangement of microtubules and microfilaments, collectively referred to as the microtubule-microfflament or microfibrillar system (32), as being responsible for these morphological changes. During RT, microtubules increase in number and become parallel to both the long axis of the cell and 6-nm microfflaments (actincontaining). These microfilaments become oriented into thick subplasmalemmal bundles (25,31). In addition, the blebbing or knob activity caused by a breakdown in the structural rigidity of microtubules and a disordered microfilament contraction and relaxation ceases (25). In further support of the observation that the microfibrillar system plays a key role in the shape changes, the RT response can be prevented or reversed by agents that depolymerize microtubules and mit This is contribution no. 364 from the Eleanor Roosevelt

Dibutyryl cAMP inhibits expression of transformation-related properties in Kirsten murine sarcoma virus transformed Balb/c-3T3 cells despite continued presence of p21v-Ki-ras

“…Alteration or dissolution of the fibrous components of the cytoskeleton has been frequently associated with transformation (Pastan and Willingham 1978;Puck 1977;Meek 1982;Puck et al 1981) and occurs rapidly following a temperature shift from nonpermissive to permissive in tsRSV-transformed cells (Edelman and Biochem. Cell Biol.…”

“…In vole cells infected with ASV, exposure to dbcAMP causes changes in growth characteristics, morphology, cytoskeletal structure, and fibronectin deposition (Puck et al 1981;Meek 1982).…”