The purpose of this study was to look into how adding Carica papaya essential oil to the diets affects the blood chemistry of broiler chickens. An 8-week trial involved 400 one-day-old mixed-sex Ross 307 chicks. The birds were divided into 4 groups, each of which contained 100 birds with 5 replicates, each containing 20 birds. Treatments T1, T2, T3, and T4 received a basal diet of 100 mg, 200 mg, and 300 mg/kg, respectively, of Carica papaya essential oil in place of T1's basal diet. Ad libitum feed and clean water were provided. The findings showed that the treatments had no appreciable impact on pack cell volume, red blood cells, hemoglobin, white blood cells, lymphocytes, monocytes, eosinophils and neutrophils values in the starter and finisher phases. Carica papaya essential oil had no discernible effect on the levels of total protein, albumin, globulin, creatinine, aspartate transaminase, or alanine phosphatase, except for cholesterol levels, which were greater in T1 than in other treatments in both the starter and finisher phases. All readings, however, fall within the ideal ranges for healthy birds, indicating no signs of infection, inflammation, or metabolic disease. The research shows that Carica papaya essential oil has several bioactive components with therapeutic value and can be used up to 300 mg/kg of diet without having any negative effects on the birds' blood profile or general performance.